Intestinal Bacteroides sp. Imbalance Associated With the Occurrence of Childhood Undernutrition in China
Undernutrition (UN) is a worldwide concern affecting morbidity and mortality among children, but the safety and long-term efficacy of its current treatments remain controversial. Recent evidence showing the roles of the gut microbiome (GM) in nutrient absorption indicates its usefulness in alternati...
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Frontiers Media S.A.
2019-11-01
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Series: | Frontiers in Microbiology |
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Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2019.02635/full |
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doaj-290a0f91dd0c4a8f985b6b2f5e22cd92 |
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record_format |
Article |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Dongfang Li Dongfang Li Yinhu Li Wenkui Dai Huihui Wang Chuangzhao Qiu Su Feng Qian Zhou Wenjian Wang Xin Feng Kaihu Yao Yanhong Liu Yonghong Yang Yonghong Yang Yonghong Yang Zhenyu Yang Ximing Xu Shuaicheng Li Jurong Wei Ke Zhou |
spellingShingle |
Dongfang Li Dongfang Li Yinhu Li Wenkui Dai Huihui Wang Chuangzhao Qiu Su Feng Qian Zhou Wenjian Wang Xin Feng Kaihu Yao Yanhong Liu Yonghong Yang Yonghong Yang Yonghong Yang Zhenyu Yang Ximing Xu Shuaicheng Li Jurong Wei Ke Zhou Intestinal Bacteroides sp. Imbalance Associated With the Occurrence of Childhood Undernutrition in China Frontiers in Microbiology childhood undernutrition gut microbiome markers Bacteroides iron transporter nutritional indicators |
author_facet |
Dongfang Li Dongfang Li Yinhu Li Wenkui Dai Huihui Wang Chuangzhao Qiu Su Feng Qian Zhou Wenjian Wang Xin Feng Kaihu Yao Yanhong Liu Yonghong Yang Yonghong Yang Yonghong Yang Zhenyu Yang Ximing Xu Shuaicheng Li Jurong Wei Ke Zhou |
author_sort |
Dongfang Li |
title |
Intestinal Bacteroides sp. Imbalance Associated With the Occurrence of Childhood Undernutrition in China |
title_short |
Intestinal Bacteroides sp. Imbalance Associated With the Occurrence of Childhood Undernutrition in China |
title_full |
Intestinal Bacteroides sp. Imbalance Associated With the Occurrence of Childhood Undernutrition in China |
title_fullStr |
Intestinal Bacteroides sp. Imbalance Associated With the Occurrence of Childhood Undernutrition in China |
title_full_unstemmed |
Intestinal Bacteroides sp. Imbalance Associated With the Occurrence of Childhood Undernutrition in China |
title_sort |
intestinal bacteroides sp. imbalance associated with the occurrence of childhood undernutrition in china |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2019-11-01 |
description |
Undernutrition (UN) is a worldwide concern affecting morbidity and mortality among children, but the safety and long-term efficacy of its current treatments remain controversial. Recent evidence showing the roles of the gut microbiome (GM) in nutrient absorption indicates its usefulness in alternative interventions to treat UN safely with sustainable amelioration. To enhance our understanding of the GM and childhood undernutrition, we deep sequenced the gut metagenomes of 65 children with moderate or severe undernutrition (UN group) and 61 healthy children (HC group) to identify associated taxa and genes using a two-stage validation scheme. At stage I, 54 UN patients and 51 healthy children were enrolled for the discovery of GM markers in UN children. The accuracy of the markers was then tested in an additional 11 UN patients and 10 healthy children at stage II. Compared to the HC group, the UN group had lower richness in microbial genes (P = 0.005, FDR = 0.005) and species (P = 0.002, FDR = 0.002). The distributions of bacterial genes enable the identification of 16 gene markers with which to discriminate UN patients with high accuracy [averaged areas under the receiver operating curve (AUC) = 0.87], including three Bacteroides uniformis genes that are responsible for the synthesis of iron transporters. We also identified four species markers that enable the UN patients to be confidently discriminated from the HC children (averaged AUC = 0.91), namely Bacteroides ovatus, Bacteroides uniformis, Bacteroides uniformis, and Bacteroides vulgatus. In addition, metabolic comparison showed significantly decreased isobutyric acid (P = 0.005, FDR = 0.017) and increased isovaleric acid (P = 0.006, FDR = 0.017) in UN patients. We also identified notable correlations between microbial species and short-chain fatty acids (SCFAs) and several nutritional indicators, including acetic acid and iron (r = 0.436, P = 0.029), butyric acid and iron (r = 0.422, P = 0.036), butyric acid and lymphocyte (r = −0.309, P = 0.011), and acetic acid and total protein (r = −0.303, P = 0.043). Taken together, the distinct features of gut microbiota in UN patients highlight the taxonomic and functional shift during the development of UN and provide a solid theoretical basis for intervention in childhood undernutrition through gut microbes. |
topic |
childhood undernutrition gut microbiome markers Bacteroides iron transporter nutritional indicators |
url |
https://www.frontiersin.org/article/10.3389/fmicb.2019.02635/full |
work_keys_str_mv |
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doaj-290a0f91dd0c4a8f985b6b2f5e22cd922020-11-25T01:23:07ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-11-011010.3389/fmicb.2019.02635490797Intestinal Bacteroides sp. Imbalance Associated With the Occurrence of Childhood Undernutrition in ChinaDongfang Li0Dongfang Li1Yinhu Li2Wenkui Dai3Huihui Wang4Chuangzhao Qiu5Su Feng6Qian Zhou7Wenjian Wang8Xin Feng9Kaihu Yao10Yanhong Liu11Yonghong Yang12Yonghong Yang13Yonghong Yang14Zhenyu Yang15Ximing Xu16Shuaicheng Li17Jurong Wei18Ke Zhou19Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Microbial Research, WeHealthGene Institute, Shenzhen, ChinaDepartment of Computer Science, College of Science and Engineering, City University of Hong Kong, Kowloon Tong, Hong KongDepartment of Microbial Research, WeHealthGene Institute, Shenzhen, ChinaDepartment of Clinical Nutrition, Shenzhen Children’s Hospital, Shenzhen, ChinaDepartment of Microbial Research, WeHealthGene Institute, Shenzhen, ChinaInstitute of Statistics, Nankai University, Tianjin, ChinaDepartment of Computer Science, College of Science and Engineering, City University of Hong Kong, Kowloon Tong, Hong KongDepartment of Respiratory Diseases, Shenzhen Children’s Hospital, Shenzhen, ChinaDepartment of Microbial Research, WeHealthGene Institute, Shenzhen, ChinaDepartment of Respiratory Diseases, Beijing Children’s Hospital, Beijing, ChinaDepartment of Microbial Research, WeHealthGene Institute, Shenzhen, ChinaDepartment of Microbial Research, WeHealthGene Institute, Shenzhen, ChinaDepartment of Respiratory Diseases, Shenzhen Children’s Hospital, Shenzhen, ChinaDepartment of Respiratory Diseases, Beijing Children’s Hospital, Beijing, ChinaDepartment of Microbial Research, WeHealthGene Institute, Shenzhen, ChinaInstitute of Statistics, Nankai University, Tianjin, ChinaDepartment of Computer Science, College of Science and Engineering, City University of Hong Kong, Kowloon Tong, Hong KongDepartment of Clinical Nutrition, Shenzhen Children’s Hospital, Shenzhen, ChinaWuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, ChinaUndernutrition (UN) is a worldwide concern affecting morbidity and mortality among children, but the safety and long-term efficacy of its current treatments remain controversial. Recent evidence showing the roles of the gut microbiome (GM) in nutrient absorption indicates its usefulness in alternative interventions to treat UN safely with sustainable amelioration. To enhance our understanding of the GM and childhood undernutrition, we deep sequenced the gut metagenomes of 65 children with moderate or severe undernutrition (UN group) and 61 healthy children (HC group) to identify associated taxa and genes using a two-stage validation scheme. At stage I, 54 UN patients and 51 healthy children were enrolled for the discovery of GM markers in UN children. The accuracy of the markers was then tested in an additional 11 UN patients and 10 healthy children at stage II. Compared to the HC group, the UN group had lower richness in microbial genes (P = 0.005, FDR = 0.005) and species (P = 0.002, FDR = 0.002). The distributions of bacterial genes enable the identification of 16 gene markers with which to discriminate UN patients with high accuracy [averaged areas under the receiver operating curve (AUC) = 0.87], including three Bacteroides uniformis genes that are responsible for the synthesis of iron transporters. We also identified four species markers that enable the UN patients to be confidently discriminated from the HC children (averaged AUC = 0.91), namely Bacteroides ovatus, Bacteroides uniformis, Bacteroides uniformis, and Bacteroides vulgatus. In addition, metabolic comparison showed significantly decreased isobutyric acid (P = 0.005, FDR = 0.017) and increased isovaleric acid (P = 0.006, FDR = 0.017) in UN patients. We also identified notable correlations between microbial species and short-chain fatty acids (SCFAs) and several nutritional indicators, including acetic acid and iron (r = 0.436, P = 0.029), butyric acid and iron (r = 0.422, P = 0.036), butyric acid and lymphocyte (r = −0.309, P = 0.011), and acetic acid and total protein (r = −0.303, P = 0.043). Taken together, the distinct features of gut microbiota in UN patients highlight the taxonomic and functional shift during the development of UN and provide a solid theoretical basis for intervention in childhood undernutrition through gut microbes.https://www.frontiersin.org/article/10.3389/fmicb.2019.02635/fullchildhood undernutritiongut microbiome markersBacteroidesiron transporternutritional indicators |