Antimicrobial Resistance and Molecular Characterization of Salmonella Enterica Serotypes Isolated from Poultry Sources in Brazil
ABSTRACT Salmonella spp. remain among the most important agents of foodborne diseases worldwide. The importance of Salmonella spp. in public health is linked to their wide range of antimicrobial resistance and to their pathogenicity and virulence in both human and animal hosts. The aim of this study...
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Fundação APINCO de Ciência e Tecnologia Avícolas
2019-04-01
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doaj-2914e66c4a99431fb5dae80f2fb97ad32020-11-25T02:14:47ZengFundação APINCO de Ciência e Tecnologia AvícolasBrazilian Journal of Poultry Science1806-90612019-04-0121110.1590/1806-9061-2018-0827S1516-635X2019000100500Antimicrobial Resistance and Molecular Characterization of Salmonella Enterica Serotypes Isolated from Poultry Sources in BrazilKA BorgesTQ FurianSN SouzaCTP SalleHLS MoraesVP NascimentoABSTRACT Salmonella spp. remain among the most important agents of foodborne diseases worldwide. The importance of Salmonella spp. in public health is linked to their wide range of antimicrobial resistance and to their pathogenicity and virulence in both human and animal hosts. The aim of this study was to determine the antimicrobial resistance patterns for Salmonella serotypes isolated from poultry sources in Brazil and to detect virulence-associated genes and verify their association with specific serotypes. A total of 163 strains of Salmonella enterica isolated from poultry sources in Southern Brazil were selected, and each belonged to one of 11 different serotypes. They were tested against ten antibiotics and examined for the presence of 26 virulence-associated genes by PCR. S. Typhimurium, S. Bredeney, S. Schwarzengrund and S. Tennessee showed the highest overall resistance rates. Approximately 18% of Salmonella strains were classified as multidrug-resistant strains. Our results indicate associations between antimicrobial resistance and specific serotypes. Most of the investigated genes presented a high frequency and a regular distribution, regardless of the serotype. Eight genes are positively or negatively associated with at least one serotype. The observed associations between antimicrobial resistance and specific serotypes are useful in developing specific control and treatment measures for each serotype. Despite the virulence genes being evenly distributed among the serotypes, some of these genes are associated with specific serotypes, and sefA, sopEand lpfA were selected as possible markers of Salmonella serotypes.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000100500&lng=en&tlng=enAntibiotic susceptibilitygenetic virulencechickensalmonellosisserovars |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
KA Borges TQ Furian SN Souza CTP Salle HLS Moraes VP Nascimento |
spellingShingle |
KA Borges TQ Furian SN Souza CTP Salle HLS Moraes VP Nascimento Antimicrobial Resistance and Molecular Characterization of Salmonella Enterica Serotypes Isolated from Poultry Sources in Brazil Brazilian Journal of Poultry Science Antibiotic susceptibility genetic virulence chicken salmonellosis serovars |
author_facet |
KA Borges TQ Furian SN Souza CTP Salle HLS Moraes VP Nascimento |
author_sort |
KA Borges |
title |
Antimicrobial Resistance and Molecular Characterization of Salmonella Enterica Serotypes Isolated from Poultry Sources in Brazil |
title_short |
Antimicrobial Resistance and Molecular Characterization of Salmonella Enterica Serotypes Isolated from Poultry Sources in Brazil |
title_full |
Antimicrobial Resistance and Molecular Characterization of Salmonella Enterica Serotypes Isolated from Poultry Sources in Brazil |
title_fullStr |
Antimicrobial Resistance and Molecular Characterization of Salmonella Enterica Serotypes Isolated from Poultry Sources in Brazil |
title_full_unstemmed |
Antimicrobial Resistance and Molecular Characterization of Salmonella Enterica Serotypes Isolated from Poultry Sources in Brazil |
title_sort |
antimicrobial resistance and molecular characterization of salmonella enterica serotypes isolated from poultry sources in brazil |
publisher |
Fundação APINCO de Ciência e Tecnologia Avícolas |
series |
Brazilian Journal of Poultry Science |
issn |
1806-9061 |
publishDate |
2019-04-01 |
description |
ABSTRACT Salmonella spp. remain among the most important agents of foodborne diseases worldwide. The importance of Salmonella spp. in public health is linked to their wide range of antimicrobial resistance and to their pathogenicity and virulence in both human and animal hosts. The aim of this study was to determine the antimicrobial resistance patterns for Salmonella serotypes isolated from poultry sources in Brazil and to detect virulence-associated genes and verify their association with specific serotypes. A total of 163 strains of Salmonella enterica isolated from poultry sources in Southern Brazil were selected, and each belonged to one of 11 different serotypes. They were tested against ten antibiotics and examined for the presence of 26 virulence-associated genes by PCR. S. Typhimurium, S. Bredeney, S. Schwarzengrund and S. Tennessee showed the highest overall resistance rates. Approximately 18% of Salmonella strains were classified as multidrug-resistant strains. Our results indicate associations between antimicrobial resistance and specific serotypes. Most of the investigated genes presented a high frequency and a regular distribution, regardless of the serotype. Eight genes are positively or negatively associated with at least one serotype. The observed associations between antimicrobial resistance and specific serotypes are useful in developing specific control and treatment measures for each serotype. Despite the virulence genes being evenly distributed among the serotypes, some of these genes are associated with specific serotypes, and sefA, sopEand lpfA were selected as possible markers of Salmonella serotypes. |
topic |
Antibiotic susceptibility genetic virulence chicken salmonellosis serovars |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000100500&lng=en&tlng=en |
work_keys_str_mv |
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1724899693552467968 |