Quantifying Live Microbial Load in Human Saliva Samples over Time Reveals Stable Composition and Dynamic Load
Human microbiomes are dynamic ecosystems often composed of hundreds of unique microbial taxa. To detect fluctuations over time in the human oral microbiome, we developed a novel workflow to quantify live microbial cells with flow cytometry in parallel with next-generation sequencing, and...
Main Authors: | , , , , , , |
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Format: | Article |
Language: | English |
Published: |
American Society for Microbiology
2021-02-01
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Series: | mSystems |
Online Access: | https://journals.asm.org/doi/10.1128/mSystems.01182-20 |
Summary: | Human microbiomes are dynamic ecosystems often composed of hundreds of unique microbial taxa. To detect fluctuations over time in the human oral microbiome, we developed a novel workflow to quantify live microbial cells with flow cytometry in parallel with next-generation sequencing, and applied this method to over 150 unstimulated, timed saliva samples. |
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ISSN: | 2379-5077 |