Association of blaNDM-1 with blaKPC-2 and aminoglycoside-modifying enzyme genes among Klebsiella pneumoniae, Proteus mirabilis and Serratia marcescens clinical isolates in Brazil
Objectives: Carbapenemase-producing Enterobacterales are frequently involved in healthcare-associated infections worldwide. The objectives of this study were to investigate (i) the frequency of the main genes encoding carbapenemases, 16S rRNA methylases and aminoglycoside-modifying enzymes (AMEs) as...
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2020-06-01
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doaj-2975e75eb84a409f844e3e622fcd7a292021-05-20T07:48:42ZengElsevierJournal of Global Antimicrobial Resistance2213-71652020-06-0121255261Association of blaNDM-1 with blaKPC-2 and aminoglycoside-modifying enzyme genes among Klebsiella pneumoniae, Proteus mirabilis and Serratia marcescens clinical isolates in BrazilElza Ferreira Firmo0Elizabeth Maria Bispo Beltrão1Felipe Rogério Ferreira da Silva2Luis Carlos Alves3Fábio André Brayner4Dyana Leal Veras5Ana Catarina Souza Lopes6Departamento de Medicina Tropical, Universidade Federal de Pernambuco (UFPE), Recife-PE, BrazilDepartamento de Medicina Tropical, Universidade Federal de Pernambuco (UFPE), Recife-PE, BrazilServiço de Patologia Clínica da Secretaria de Saúde de Pernambuco, Recife-PE, BrazilDepartamento de Parasitologia, Instituto Aggeu Magalhães – IAM/FIOCRUZ-PE, Recife, PE, BrazilDepartamento de Parasitologia, Instituto Aggeu Magalhães – IAM/FIOCRUZ-PE, Recife, PE, BrazilDepartamento de Parasitologia, Instituto Aggeu Magalhães – IAM/FIOCRUZ-PE, Recife, PE, BrazilDepartamento de Medicina Tropical, Universidade Federal de Pernambuco (UFPE), Recife-PE, Brazil; Corresponding author. Present address: Universidade Federal de Pernambuco (UFPE), Centro de Ciências Médicas (CCM), Área de Medicina Tropical, Av. Prof. Moraes Rego, s/n, Recife, PE 50.732-970, Brazil.Objectives: Carbapenemase-producing Enterobacterales are frequently involved in healthcare-associated infections worldwide. The objectives of this study were to investigate (i) the frequency of the main genes encoding carbapenemases, 16S rRNA methylases and aminoglycoside-modifying enzymes (AMEs) as well as the mcr gene and (ii) the clonal relationship of enterobacteria isolates resistant to carbapenems and aminoglycosides from colonisation and infection in patients from hospitals in northeastern Brazil. Methods: Antimicrobial susceptibility was determined using an automated VITEK®2 system. Presence of carbapenemase, AME and 16S rRNA methylase genes as well as the mcr gene was determined by PCR and amplicon sequencing. Genetic variability was determined by ERIC-PCR. Results: A total of 35 isolates resistant to carbapenems and aminoglycosides were selected for this study. Klebsiella pneumoniae was most common (45.7%), followed by Proteus mirabilis (28.6%) and Serratia marcescens (25.7%). AME genes were found in 97.1% of isolates, most commonly aph(3ʹ)-VI and aac(6')-Ib. The blaNDM-1 and blaKPC-2 genes were detected in 25.7% and 88.6% of isolates, respectively; five isolates harboured these genes concomitantly. According to the literature, this is the first report of the association of blaNDM-1 and blaKPC-2 in P. mirabilis and S. marcescens in Brazil. The isolates showed a multiclonal profile by ERIC-PCR. Conclusion: The emergence of blaNDM-1 associated with blaKPC-2 and AME genes in K. pneumoniae, P. mirabilis and S. marcescens isolates with a multiclonal profile is of concern as this limits therapeutic options. These results should alert medical authorities to establish rigorous detection methods to reduce the spread of these antimicrobial resistance genes.http://www.sciencedirect.com/science/article/pii/S2213716519302322EnterobacteralesNDMKPCAminoglycoside-modifying enzyme |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Elza Ferreira Firmo Elizabeth Maria Bispo Beltrão Felipe Rogério Ferreira da Silva Luis Carlos Alves Fábio André Brayner Dyana Leal Veras Ana Catarina Souza Lopes |
spellingShingle |
Elza Ferreira Firmo Elizabeth Maria Bispo Beltrão Felipe Rogério Ferreira da Silva Luis Carlos Alves Fábio André Brayner Dyana Leal Veras Ana Catarina Souza Lopes Association of blaNDM-1 with blaKPC-2 and aminoglycoside-modifying enzyme genes among Klebsiella pneumoniae, Proteus mirabilis and Serratia marcescens clinical isolates in Brazil Journal of Global Antimicrobial Resistance Enterobacterales NDM KPC Aminoglycoside-modifying enzyme |
author_facet |
Elza Ferreira Firmo Elizabeth Maria Bispo Beltrão Felipe Rogério Ferreira da Silva Luis Carlos Alves Fábio André Brayner Dyana Leal Veras Ana Catarina Souza Lopes |
author_sort |
Elza Ferreira Firmo |
title |
Association of blaNDM-1 with blaKPC-2 and aminoglycoside-modifying enzyme genes among Klebsiella pneumoniae, Proteus mirabilis and Serratia marcescens clinical isolates in Brazil |
title_short |
Association of blaNDM-1 with blaKPC-2 and aminoglycoside-modifying enzyme genes among Klebsiella pneumoniae, Proteus mirabilis and Serratia marcescens clinical isolates in Brazil |
title_full |
Association of blaNDM-1 with blaKPC-2 and aminoglycoside-modifying enzyme genes among Klebsiella pneumoniae, Proteus mirabilis and Serratia marcescens clinical isolates in Brazil |
title_fullStr |
Association of blaNDM-1 with blaKPC-2 and aminoglycoside-modifying enzyme genes among Klebsiella pneumoniae, Proteus mirabilis and Serratia marcescens clinical isolates in Brazil |
title_full_unstemmed |
Association of blaNDM-1 with blaKPC-2 and aminoglycoside-modifying enzyme genes among Klebsiella pneumoniae, Proteus mirabilis and Serratia marcescens clinical isolates in Brazil |
title_sort |
association of blandm-1 with blakpc-2 and aminoglycoside-modifying enzyme genes among klebsiella pneumoniae, proteus mirabilis and serratia marcescens clinical isolates in brazil |
publisher |
Elsevier |
series |
Journal of Global Antimicrobial Resistance |
issn |
2213-7165 |
publishDate |
2020-06-01 |
description |
Objectives: Carbapenemase-producing Enterobacterales are frequently involved in healthcare-associated infections worldwide. The objectives of this study were to investigate (i) the frequency of the main genes encoding carbapenemases, 16S rRNA methylases and aminoglycoside-modifying enzymes (AMEs) as well as the mcr gene and (ii) the clonal relationship of enterobacteria isolates resistant to carbapenems and aminoglycosides from colonisation and infection in patients from hospitals in northeastern Brazil. Methods: Antimicrobial susceptibility was determined using an automated VITEK®2 system. Presence of carbapenemase, AME and 16S rRNA methylase genes as well as the mcr gene was determined by PCR and amplicon sequencing. Genetic variability was determined by ERIC-PCR. Results: A total of 35 isolates resistant to carbapenems and aminoglycosides were selected for this study. Klebsiella pneumoniae was most common (45.7%), followed by Proteus mirabilis (28.6%) and Serratia marcescens (25.7%). AME genes were found in 97.1% of isolates, most commonly aph(3ʹ)-VI and aac(6')-Ib. The blaNDM-1 and blaKPC-2 genes were detected in 25.7% and 88.6% of isolates, respectively; five isolates harboured these genes concomitantly. According to the literature, this is the first report of the association of blaNDM-1 and blaKPC-2 in P. mirabilis and S. marcescens in Brazil. The isolates showed a multiclonal profile by ERIC-PCR. Conclusion: The emergence of blaNDM-1 associated with blaKPC-2 and AME genes in K. pneumoniae, P. mirabilis and S. marcescens isolates with a multiclonal profile is of concern as this limits therapeutic options. These results should alert medical authorities to establish rigorous detection methods to reduce the spread of these antimicrobial resistance genes. |
topic |
Enterobacterales NDM KPC Aminoglycoside-modifying enzyme |
url |
http://www.sciencedirect.com/science/article/pii/S2213716519302322 |
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