Dynamics of Urinary Calprotectin after Renal Ischaemia.
BACKGROUND:Urinary calprotectin has been identified as a promising biomarker for acute kidney injury. To date, however, the time-dependent changes of this parameter during acute kidney injury remain elusive. The aim of the present work was to define the time-course of urinary calprotectin secretion...
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doaj-297f4f7492824f10913f56223e9a04c42020-11-25T01:24:59ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01111e014639510.1371/journal.pone.0146395Dynamics of Urinary Calprotectin after Renal Ischaemia.Jan EbbingFelix S SeibertNikolaos PagonasFrederic BauerKurt MillerCarsten KempkensteffenKarsten GünzelAlexander BachmannHans H SeifertCyrill A RentschPeter ArdeltChristian WetterauerPatrizia AmicoNina BabelTimm H WesthoffBACKGROUND:Urinary calprotectin has been identified as a promising biomarker for acute kidney injury. To date, however, the time-dependent changes of this parameter during acute kidney injury remain elusive. The aim of the present work was to define the time-course of urinary calprotectin secretion after ischaemia/reperfusion-induced kidney injury in comparison to neutrophil gelatinase-associated lipocalin, thereby monitoring the extent of tubular damage in nephron sparing surgery for kidney tumours. METHODS:The study population consisted of 42 patients. Thirty-two patients underwent either open or endoscopic nephron sparing surgery for kidney tumours. During the surgery, the renal arterial pedicle was clamped with a median ischaemic time of 13 minutes (interquartile range, 4.5-20.3 minutes) in 26 patients. Ten retro-peritoneoscopic living donor nephrectomy patients and 6 nephron sparing surgery patients in whom the renal artery was not clamped served as controls. Urinary calprotectin and neutrophil gelatinase-associated lipocalin concentrations were repeatedly measured by enzyme-linked immunosorbent assay and assessed according to renal function parameters. RESULTS:Urinary concentrations of calprotectin and neutrophil gelatinase-associated lipocalin increased significantly after ischaemia/reperfusion injury, whereas concentrations remained unchanged after nephron sparing surgery without ischaemia/reperfusion injury and after kidney donation. Calprotectin and neutrophil gelatinase-associated lipocalin levels were significantly increased 2 and 8 hours, respectively, post-ischaemia. Both proteins reached maximal concentrations after 48 hours, followed by a subsequent persistent decrease. Maximal neutrophil gelatinase-associated lipocalin and calprotectin concentrations were 9-fold and 69-fold higher than their respective baseline values. The glomerular filtration rate was only transiently impaired at the first post-operative day after ischaemia/reperfusion injury (p = 0.049). CONCLUSION:Calprotectin and neutrophil gelatinase-associated lipocalin can be used to monitor clinical and sub-clinical tubular damage after nephron sparing surgery for kidney tumours. Urinary calprotectin concentrations start rising within 2 hours after ischaemia/reperfusion-induced kidney injury.http://europepmc.org/articles/PMC4706321?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jan Ebbing Felix S Seibert Nikolaos Pagonas Frederic Bauer Kurt Miller Carsten Kempkensteffen Karsten Günzel Alexander Bachmann Hans H Seifert Cyrill A Rentsch Peter Ardelt Christian Wetterauer Patrizia Amico Nina Babel Timm H Westhoff |
spellingShingle |
Jan Ebbing Felix S Seibert Nikolaos Pagonas Frederic Bauer Kurt Miller Carsten Kempkensteffen Karsten Günzel Alexander Bachmann Hans H Seifert Cyrill A Rentsch Peter Ardelt Christian Wetterauer Patrizia Amico Nina Babel Timm H Westhoff Dynamics of Urinary Calprotectin after Renal Ischaemia. PLoS ONE |
author_facet |
Jan Ebbing Felix S Seibert Nikolaos Pagonas Frederic Bauer Kurt Miller Carsten Kempkensteffen Karsten Günzel Alexander Bachmann Hans H Seifert Cyrill A Rentsch Peter Ardelt Christian Wetterauer Patrizia Amico Nina Babel Timm H Westhoff |
author_sort |
Jan Ebbing |
title |
Dynamics of Urinary Calprotectin after Renal Ischaemia. |
title_short |
Dynamics of Urinary Calprotectin after Renal Ischaemia. |
title_full |
Dynamics of Urinary Calprotectin after Renal Ischaemia. |
title_fullStr |
Dynamics of Urinary Calprotectin after Renal Ischaemia. |
title_full_unstemmed |
Dynamics of Urinary Calprotectin after Renal Ischaemia. |
title_sort |
dynamics of urinary calprotectin after renal ischaemia. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2016-01-01 |
description |
BACKGROUND:Urinary calprotectin has been identified as a promising biomarker for acute kidney injury. To date, however, the time-dependent changes of this parameter during acute kidney injury remain elusive. The aim of the present work was to define the time-course of urinary calprotectin secretion after ischaemia/reperfusion-induced kidney injury in comparison to neutrophil gelatinase-associated lipocalin, thereby monitoring the extent of tubular damage in nephron sparing surgery for kidney tumours. METHODS:The study population consisted of 42 patients. Thirty-two patients underwent either open or endoscopic nephron sparing surgery for kidney tumours. During the surgery, the renal arterial pedicle was clamped with a median ischaemic time of 13 minutes (interquartile range, 4.5-20.3 minutes) in 26 patients. Ten retro-peritoneoscopic living donor nephrectomy patients and 6 nephron sparing surgery patients in whom the renal artery was not clamped served as controls. Urinary calprotectin and neutrophil gelatinase-associated lipocalin concentrations were repeatedly measured by enzyme-linked immunosorbent assay and assessed according to renal function parameters. RESULTS:Urinary concentrations of calprotectin and neutrophil gelatinase-associated lipocalin increased significantly after ischaemia/reperfusion injury, whereas concentrations remained unchanged after nephron sparing surgery without ischaemia/reperfusion injury and after kidney donation. Calprotectin and neutrophil gelatinase-associated lipocalin levels were significantly increased 2 and 8 hours, respectively, post-ischaemia. Both proteins reached maximal concentrations after 48 hours, followed by a subsequent persistent decrease. Maximal neutrophil gelatinase-associated lipocalin and calprotectin concentrations were 9-fold and 69-fold higher than their respective baseline values. The glomerular filtration rate was only transiently impaired at the first post-operative day after ischaemia/reperfusion injury (p = 0.049). CONCLUSION:Calprotectin and neutrophil gelatinase-associated lipocalin can be used to monitor clinical and sub-clinical tubular damage after nephron sparing surgery for kidney tumours. Urinary calprotectin concentrations start rising within 2 hours after ischaemia/reperfusion-induced kidney injury. |
url |
http://europepmc.org/articles/PMC4706321?pdf=render |
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