Amino Acid Derivatives of Ligustrazine-Oleanolic Acid as New Cytotoxic Agents
A series of novel ligustrazine-oleanolic acid (TOA) derivatives were designed, and synthesized by conjugating amino acids to the 3-hydroxy group of TOA by ester bonds. Their cytotoxicity was evaluated on four cancer cell lines (HepG2, HT-29, Hela and BGC-823) by standard MTT assays. The ClogP value...
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doaj-29845d712a3f46bcaac61c0ae031d6ba2020-11-24T20:51:46ZengMDPI AGMolecules1420-30492014-11-011911182151823110.3390/molecules191118215molecules191118215Amino Acid Derivatives of Ligustrazine-Oleanolic Acid as New Cytotoxic AgentsFuhao Chu0Xin Xu1Guoliang Li2Shun Gu3Kuo Xu4Yan Gong5Bing Xu6Mina Wang7Huazheng Zhang8Yuzhong Zhang9Penglong Wang10Haimin Lei11School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, ChinaSchool of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, ChinaSchool of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, ChinaKey Laboratory for Neurodegenerative Diseases of Ministry of Education, Xuanwu Hospital of Capital Medical University, Beijing 100053, ChinaSchool of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, ChinaSchool of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, ChinaSchool of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, ChinaSchool of Basic Medicine, Beijing University of Chinese Medicine, Beijing 100029, ChinaSchool of Basic Medicine, Beijing University of Chinese Medicine, Beijing 100029, ChinaSchool of Basic Medicine, Beijing University of Chinese Medicine, Beijing 100029, ChinaSchool of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, ChinaSchool of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, ChinaA series of novel ligustrazine-oleanolic acid (TOA) derivatives were designed, and synthesized by conjugating amino acids to the 3-hydroxy group of TOA by ester bonds. Their cytotoxicity was evaluated on four cancer cell lines (HepG2, HT-29, Hela and BGC-823) by standard MTT assays. The ClogP values were calculated by means of computer simulation, and logP values of both 3β-glycine ester olean-12-en-28-oic acid-3,5,6-trimethylpyrazin-2-methyl ester (6a) and TOA were determined using a shake flask-ultraviolet spectrophotometry method. It was found that 6a and the 3β-L-lysine ester-6g not only displayed good cytotoxicity (IC50 < 3.5 μM) but also possessed better hydrophilicity than TOA. Moreover, 6a (IC50 = 4.884 μM) had lower nephrotoxicity than both 6g (IC50 = 2.310 μM) and cisplatin (CDDP, IC50 = 3.691 μM) on MDCK cells. Combining Giemsa and DAPI staining, it was further verified that 6a could induce HepG2 apoptosis via nuclei fragmentation and had lower nephrotoxicity. In addition, the structure-activity relationships of these derivatives are briefly discussed.http://www.mdpi.com/1420-3049/19/11/18215amino acidligustrazine-oleanolic acidanticancerClogPlow toxicityGiemsa and DAPI staining |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Fuhao Chu Xin Xu Guoliang Li Shun Gu Kuo Xu Yan Gong Bing Xu Mina Wang Huazheng Zhang Yuzhong Zhang Penglong Wang Haimin Lei |
spellingShingle |
Fuhao Chu Xin Xu Guoliang Li Shun Gu Kuo Xu Yan Gong Bing Xu Mina Wang Huazheng Zhang Yuzhong Zhang Penglong Wang Haimin Lei Amino Acid Derivatives of Ligustrazine-Oleanolic Acid as New Cytotoxic Agents Molecules amino acid ligustrazine-oleanolic acid anticancer ClogP low toxicity Giemsa and DAPI staining |
author_facet |
Fuhao Chu Xin Xu Guoliang Li Shun Gu Kuo Xu Yan Gong Bing Xu Mina Wang Huazheng Zhang Yuzhong Zhang Penglong Wang Haimin Lei |
author_sort |
Fuhao Chu |
title |
Amino Acid Derivatives of Ligustrazine-Oleanolic Acid as New Cytotoxic Agents |
title_short |
Amino Acid Derivatives of Ligustrazine-Oleanolic Acid as New Cytotoxic Agents |
title_full |
Amino Acid Derivatives of Ligustrazine-Oleanolic Acid as New Cytotoxic Agents |
title_fullStr |
Amino Acid Derivatives of Ligustrazine-Oleanolic Acid as New Cytotoxic Agents |
title_full_unstemmed |
Amino Acid Derivatives of Ligustrazine-Oleanolic Acid as New Cytotoxic Agents |
title_sort |
amino acid derivatives of ligustrazine-oleanolic acid as new cytotoxic agents |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2014-11-01 |
description |
A series of novel ligustrazine-oleanolic acid (TOA) derivatives were designed, and synthesized by conjugating amino acids to the 3-hydroxy group of TOA by ester bonds. Their cytotoxicity was evaluated on four cancer cell lines (HepG2, HT-29, Hela and BGC-823) by standard MTT assays. The ClogP values were calculated by means of computer simulation, and logP values of both 3β-glycine ester olean-12-en-28-oic acid-3,5,6-trimethylpyrazin-2-methyl ester (6a) and TOA were determined using a shake flask-ultraviolet spectrophotometry method. It was found that 6a and the 3β-L-lysine ester-6g not only displayed good cytotoxicity (IC50 < 3.5 μM) but also possessed better hydrophilicity than TOA. Moreover, 6a (IC50 = 4.884 μM) had lower nephrotoxicity than both 6g (IC50 = 2.310 μM) and cisplatin (CDDP, IC50 = 3.691 μM) on MDCK cells. Combining Giemsa and DAPI staining, it was further verified that 6a could induce HepG2 apoptosis via nuclei fragmentation and had lower nephrotoxicity. In addition, the structure-activity relationships of these derivatives are briefly discussed. |
topic |
amino acid ligustrazine-oleanolic acid anticancer ClogP low toxicity Giemsa and DAPI staining |
url |
http://www.mdpi.com/1420-3049/19/11/18215 |
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