Phylogenomic characterization of ranaviruses isolated from cultured fish and amphibians in Thailand
Ranaviruses are emerging pathogens associated with worldwide epizootics in farmed and wild ectothermic vertebrates. In this study, we determined the full genomes of eight ranaviruses isolated from marbled sleeper goby (Oxyeleotris marmorata), goldfish (Carassius auratus), guppy (Poecilia reticulata)...
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doaj-299fcfebd33f4348a754279e7810f29c2021-04-08T19:55:15ZengCanadian Science PublishingFACETS2371-16712020-12-0151963 97910.1139/facets-2020-0043Phylogenomic characterization of ranaviruses isolated from cultured fish and amphibians in ThailandPreeyanan Sriwanayos0Kuttichantran Subramaniam1Natalie K. Stilwell2Kamonchai Imnoi3Vsevolod L. Popov4Somkiat Kanchanakhan5Jaree Polchana6Thomas B. Waltzek7Department of Infectious Diseases and Immunology, College of Veterinary Medicine, University of Florida, Gainesville, FL 32611, USA Aquatic Animal Health Research and Development Division, Department of Fisheries, Bangkok 10900, ThailandDepartment of Infectious Diseases and Immunology, College of Veterinary Medicine, University of Florida, Gainesville, FL 32611, USADepartment of Infectious Diseases and Immunology, College of Veterinary Medicine, University of Florida, Gainesville, FL 32611, USA Southeastern Cooperative Wildlife Disease Study, College of Veterinary Medicine, University of Georgia, Athens, GA 30602, USADepartment of Infectious Diseases and Immunology, College of Veterinary Medicine, University of Florida, Gainesville, FL 32611, USADepartment of Pathology, University of Texas Medical Branch, Galveston, TX 77555, USAAquatic Animal Health Research and Development Division, Department of Fisheries, Bangkok 10900, Thailand Department of Fisheries, Chonburi Provincial Fishery Office, Chonburi 20000, ThailandAquatic Animal Health Research and Development Division, Department of Fisheries, Bangkok 10900, ThailandDepartment of Infectious Diseases and Immunology, College of Veterinary Medicine, University of Florida, Gainesville, FL 32611, USARanaviruses are emerging pathogens associated with worldwide epizootics in farmed and wild ectothermic vertebrates. In this study, we determined the full genomes of eight ranaviruses isolated from marbled sleeper goby (Oxyeleotris marmorata), goldfish (Carassius auratus), guppy (Poecilia reticulata), tiger frog (Hoplobatrachus tigerinus), Asian grass frog (Fejervarya limnocharis), and East Asian bullfrog (H. rugulosus) cultured or imported into Thailand. These ranaviral isolates induced the same cytopathic effects (i.e., progression of coalescing round plaques) in epithelioma papulosum cyprini (EPC) cell cultures. Transmission electron microscopy of infected EPC cells revealed cytoplasmic viral particles with ultrastructural features typical for ranaviruses. Pairwise genetic comparisons of the complete major capsid protein coding sequences from the Thai ranaviruses displayed the highest identity (99.8%–100%) to a ranavirus (tiger frog virus; TFV) isolated from diseased tiger frogs cultured in China, a slightly lower identity (99.3%–99.4%) to a ranavirus (Wamena virus; WV) isolated from diseased green tree pythons (Morelia viridis) illegally exported from Papua New Guinea, and a lower identity to 35 other ranaviruses (93.7%–98.6%). Phylogenomic analyses supported the eight Thai ranaviruses, Chinese TFV, and WV as a subclade within a larger frog virus 3 clade. Our findings confirm the spread of TFV among cultured fish and amphibians in Asia and likely in reptiles in Oceania. Biosecurity measures are needed to ensure TFV does not continue to spread throughout Southeast Asia and to other parts of the world via international trade.https://www.facetsjournal.com/doi/full/10.1139/facets-2020-0043iridovirusphylogenomicsranavirusthailandtiger frog virus |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Preeyanan Sriwanayos Kuttichantran Subramaniam Natalie K. Stilwell Kamonchai Imnoi Vsevolod L. Popov Somkiat Kanchanakhan Jaree Polchana Thomas B. Waltzek |
spellingShingle |
Preeyanan Sriwanayos Kuttichantran Subramaniam Natalie K. Stilwell Kamonchai Imnoi Vsevolod L. Popov Somkiat Kanchanakhan Jaree Polchana Thomas B. Waltzek Phylogenomic characterization of ranaviruses isolated from cultured fish and amphibians in Thailand FACETS iridovirus phylogenomics ranavirus thailand tiger frog virus |
author_facet |
Preeyanan Sriwanayos Kuttichantran Subramaniam Natalie K. Stilwell Kamonchai Imnoi Vsevolod L. Popov Somkiat Kanchanakhan Jaree Polchana Thomas B. Waltzek |
author_sort |
Preeyanan Sriwanayos |
title |
Phylogenomic characterization of ranaviruses isolated from cultured fish and amphibians in Thailand |
title_short |
Phylogenomic characterization of ranaviruses isolated from cultured fish and amphibians in Thailand |
title_full |
Phylogenomic characterization of ranaviruses isolated from cultured fish and amphibians in Thailand |
title_fullStr |
Phylogenomic characterization of ranaviruses isolated from cultured fish and amphibians in Thailand |
title_full_unstemmed |
Phylogenomic characterization of ranaviruses isolated from cultured fish and amphibians in Thailand |
title_sort |
phylogenomic characterization of ranaviruses isolated from cultured fish and amphibians in thailand |
publisher |
Canadian Science Publishing |
series |
FACETS |
issn |
2371-1671 |
publishDate |
2020-12-01 |
description |
Ranaviruses are emerging pathogens associated with worldwide epizootics in farmed and wild ectothermic vertebrates. In this study, we determined the full genomes of eight ranaviruses isolated from marbled sleeper goby (Oxyeleotris marmorata), goldfish (Carassius auratus), guppy (Poecilia reticulata), tiger frog (Hoplobatrachus tigerinus), Asian grass frog (Fejervarya limnocharis), and East Asian bullfrog (H. rugulosus) cultured or imported into Thailand. These ranaviral isolates induced the same cytopathic effects (i.e., progression of coalescing round plaques) in epithelioma papulosum cyprini (EPC) cell cultures. Transmission electron microscopy of infected EPC cells revealed cytoplasmic viral particles with ultrastructural features typical for ranaviruses. Pairwise genetic comparisons of the complete major capsid protein coding sequences from the Thai ranaviruses displayed the highest identity (99.8%–100%) to a ranavirus (tiger frog virus; TFV) isolated from diseased tiger frogs cultured in China, a slightly lower identity (99.3%–99.4%) to a ranavirus (Wamena virus; WV) isolated from diseased green tree pythons (Morelia viridis) illegally exported from Papua New Guinea, and a lower identity to 35 other ranaviruses (93.7%–98.6%). Phylogenomic analyses supported the eight Thai ranaviruses, Chinese TFV, and WV as a subclade within a larger frog virus 3 clade. Our findings confirm the spread of TFV among cultured fish and amphibians in Asia and likely in reptiles in Oceania. Biosecurity measures are needed to ensure TFV does not continue to spread throughout Southeast Asia and to other parts of the world via international trade. |
topic |
iridovirus phylogenomics ranavirus thailand tiger frog virus |
url |
https://www.facetsjournal.com/doi/full/10.1139/facets-2020-0043 |
work_keys_str_mv |
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