The roots of <it>Atractylodes japonica</it> Koidzumi promote adipogenic differentiation via activation of the insulin signaling pathway in 3T3-L1 cells
<p>Abstract</p> <p>Background</p> <p>Type 2 diabetes (T2D) is a complex metabolic disorder characterized by insulin resistance and hyperglycemia. Peroxisome proliferator-activated receptor gamma (PPARγ) is a key transcription factor and plays an important role in the re...
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doaj-29a95ddb430e4919a60f536323ce3d702020-11-25T03:22:16ZengBMCBMC Complementary and Alternative Medicine1472-68822012-09-0112115410.1186/1472-6882-12-154The roots of <it>Atractylodes japonica</it> Koidzumi promote adipogenic differentiation via activation of the insulin signaling pathway in 3T3-L1 cellsHan YunkyungJung HyoPark Yong-Ki<p>Abstract</p> <p>Background</p> <p>Type 2 diabetes (T2D) is a complex metabolic disorder characterized by insulin resistance and hyperglycemia. Peroxisome proliferator-activated receptor gamma (PPARγ) is a key transcription factor and plays an important role in the regulation of genes involved in adipogenic differentiation, glucose metabolism and insulin signal transduction.</p> <p>Methods</p> <p>In this study, the effects of the root extract of <it>Atractylodes japonica</it> Koidzumi <b>(</b>Atractylodis Rhizoma Alba, ARA) on the differentiation of 3T3-L1 preadipocytes and the possible mechanism of glucose transport were investigated. 3T3-L1 cells were cultured with insulin and ARA extract.</p> <p>Results</p> <p>In 3T3-L1 cells, ARA extract significantly enhanced adipogenic differentiation and upregulated the expression of PPARγ genes and protein in a dose-dependent manner. ARA also promoted glucose transport by increasing the glucose transporter 4 (GLUT-4), phosphatidylinositol 3-kinase (PI3K) and insulin receptor substrates-1 (IRS-1) levels.</p> <p>Conclusion</p> <p>Our results suggest that ARA extract may be an attractive therapeutic agent for managing T2D via promoting the differentiation of adipocytes with the upregulation of PPARγ levels and the activation of the insulin signaling pathway.</p> http://www.biomedcentral.com/1472-6882/12/154<it>Atractylodes japonica</it>Atractylodis Rhizoma Alba3T3-L1 adipocytePPARγGLUT4InsulinType 2 diabetes |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Han Yunkyung Jung Hyo Park Yong-Ki |
spellingShingle |
Han Yunkyung Jung Hyo Park Yong-Ki The roots of <it>Atractylodes japonica</it> Koidzumi promote adipogenic differentiation via activation of the insulin signaling pathway in 3T3-L1 cells BMC Complementary and Alternative Medicine <it>Atractylodes japonica</it> Atractylodis Rhizoma Alba 3T3-L1 adipocyte PPARγ GLUT4 Insulin Type 2 diabetes |
author_facet |
Han Yunkyung Jung Hyo Park Yong-Ki |
author_sort |
Han Yunkyung |
title |
The roots of <it>Atractylodes japonica</it> Koidzumi promote adipogenic differentiation via activation of the insulin signaling pathway in 3T3-L1 cells |
title_short |
The roots of <it>Atractylodes japonica</it> Koidzumi promote adipogenic differentiation via activation of the insulin signaling pathway in 3T3-L1 cells |
title_full |
The roots of <it>Atractylodes japonica</it> Koidzumi promote adipogenic differentiation via activation of the insulin signaling pathway in 3T3-L1 cells |
title_fullStr |
The roots of <it>Atractylodes japonica</it> Koidzumi promote adipogenic differentiation via activation of the insulin signaling pathway in 3T3-L1 cells |
title_full_unstemmed |
The roots of <it>Atractylodes japonica</it> Koidzumi promote adipogenic differentiation via activation of the insulin signaling pathway in 3T3-L1 cells |
title_sort |
roots of <it>atractylodes japonica</it> koidzumi promote adipogenic differentiation via activation of the insulin signaling pathway in 3t3-l1 cells |
publisher |
BMC |
series |
BMC Complementary and Alternative Medicine |
issn |
1472-6882 |
publishDate |
2012-09-01 |
description |
<p>Abstract</p> <p>Background</p> <p>Type 2 diabetes (T2D) is a complex metabolic disorder characterized by insulin resistance and hyperglycemia. Peroxisome proliferator-activated receptor gamma (PPARγ) is a key transcription factor and plays an important role in the regulation of genes involved in adipogenic differentiation, glucose metabolism and insulin signal transduction.</p> <p>Methods</p> <p>In this study, the effects of the root extract of <it>Atractylodes japonica</it> Koidzumi <b>(</b>Atractylodis Rhizoma Alba, ARA) on the differentiation of 3T3-L1 preadipocytes and the possible mechanism of glucose transport were investigated. 3T3-L1 cells were cultured with insulin and ARA extract.</p> <p>Results</p> <p>In 3T3-L1 cells, ARA extract significantly enhanced adipogenic differentiation and upregulated the expression of PPARγ genes and protein in a dose-dependent manner. ARA also promoted glucose transport by increasing the glucose transporter 4 (GLUT-4), phosphatidylinositol 3-kinase (PI3K) and insulin receptor substrates-1 (IRS-1) levels.</p> <p>Conclusion</p> <p>Our results suggest that ARA extract may be an attractive therapeutic agent for managing T2D via promoting the differentiation of adipocytes with the upregulation of PPARγ levels and the activation of the insulin signaling pathway.</p> |
topic |
<it>Atractylodes japonica</it> Atractylodis Rhizoma Alba 3T3-L1 adipocyte PPARγ GLUT4 Insulin Type 2 diabetes |
url |
http://www.biomedcentral.com/1472-6882/12/154 |
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