Combined use of municipal solid waste biochar and bacterial biosorbent synergistically decreases Cd(II) and Pb(II) concentration in edible tissue of forage maize irrigated with heavy metal–spiked water

• Main Authors: Motahhareh Abedinzadeh, Hassan Etesami, Hossein Ali Alikhani, Saeid Shafiei
• Format: Article
• Language: English
• Published: Elsevier 2020-08-01
• Series: Heliyon
• Subjects: Agricultural science; Biotechnology; Microbiology environmental science; Cadmium; Enterobacter cloacae; Heavy metal immobilization
• Online Access: http://www.sciencedirect.com/science/article/pii/S2405844020315310

A pot experiment was carried out to evaluate the effect of a municipal solid waste (MSW) biochar and a bacterial strain on the forage maize growth and the concentration of lead (Pb) and cadmium (Cd) in the edible tissue of maize irrigated with water contaminated with Cd (5 mg L−1) and Pb (100 mg L−1). Experimental treatments included (i) bacterial strain at two levels: no bacterial strain and Enterobacter cloacae R7; (ii) MSW biochar at three levels: 0, 1, and 3% (w/w); and (iii) irrigation water quality at five levels: plants irrigated with 100% freshwater (FW), plants irrigated with 75%FW + 25% contaminated water (CW), plants irrigated with 50%FW + 50% CW, plants irrigated with 25%FW + 75% CW, and plants irrigated with 100% CW. The effect of various treatments on maize growth indices and concentration of Pb(II) and Cd(II) in the plant was significant at 5% level. The concentration of these metals in the shoot of plants irrigated with 75 and 100% CW was higher than the permissible limits for Cd(II) and Pb(II) in livestock feed. However, the concentration of these metals in the shoot of the plants irrigated with 25 and 50% CW was lower than the permissible limit for this use. In this study, the combined application of 3%biochar and E. cloacae R7 had a significant effect on increased root dry weight (ranging from 29 to 33%), shoot dry weight (ranging from 32 to 43%) and bacterial root colonization (ranging from 33 to 53%) and on reduced concentration of Pb (ranging from 78 to 80%) and Cd (ranging from 72 to 76%) of the shoot of maize plant (edible tissues used by livestock), which was below the permissible limits for livestock feed, compared to corresponding controls. According to the results of this study, to reduce the concentration of the heavy metals in forage maize shoot (below the permissible limits for livestock feed), it is suggested using heavy metal–contaminated water either in combination with freshwater (50 or 75% FW) or in combination with biochar and bacterial biosorbent, averting human/animal health risk.