Distribution of Antibiotic Resistance Genes among the Phylogroups of Escherichia coli in Diarrheic Calves and Chickens Affected by Colibacillosis in Tehran, Iran

• Main Authors: H. Staji, A. Tonelli, T. Zahraei Salehi, A. Mahdavi, E. Shahroozian, M. R. Salimi Bejestani, S. Mehdizade Mood, M. Keywanloo, M. Ahmadi Hamedani, H. Emadi Chashmi, I. Ashrafi Tamai, E. Atefi Tabar
• Format: Article
• Language: English
• Published: Razi Vaccine and Serum Research Institute 2018-06-01
• Series: Archives of Razi Institute
• Subjects: Escherichia coli; phylogroups; Integrons; Resistance genes
• Online Access: http://archrazi.areeo.ac.ir/article_116502_53e0a6ab410f8a4cb5e452e8af262829.pdf
• ISSN: 0365-3439; 2008-9872

Antibiotic resistance occurs in the endogenous flora of exposed population in addition to pathogenic bacteria. This study was conducted to evaluate the distribution of antibiotic resistance genes among 63 isolates of Escherichia coli of Escherichia coli (E. coli) in diarrheic calves and poultry. According to the results, B1 and B2 were the most prevalent phylogroups of E. coli in calves and poultry carcasses, respectively. Antimicrobial resistance was observed in 76% of the isolates, and 62% of the strains were multi-drug resistant. Antibiotic resistance in E. coli strains obtained from calves strains was significantly higher than those obtained from poultries. Additionally, the strains of B1 and D phylogroups had the highest and lowest antimicrobial resistance, respectively. At least one encoding gene for integrone was detected in 23 strains (36.5%) and Class I integron had the highest prevalence. Accordingly, this study gave baseline information on the magnitude of the resistance problem and its genetic background in E. coli from domesticated animals of the Tehran, Iran. Moreover, the power of oligonucleotide array technology in the discrimination of different genotypes during a short time was confirmed in this study.