Voltage gated potassium channels expressed in Xenopus laevis(AMPHIBIA) oocytes

Voltage gated potassium channels expressed in Xenopus laevis(AMPHIBIA) oocytes

Heterologous expression has been an important tool for structural and functionalcharacterization of proteins. The study of biophysical properties of ion channels,pumps and transporters has been possible thanks to their expression in Xenopuslaevisoocytes. Here we report the expression of two voltage...

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Main Authors: Hedna Chaves, Carlos Villalba, Luisa Lagos, Rafael Vargas, Francisco Martínez-Wittinghan, Carlos Clavijo, Marcela Camacho
Format: Article
Language:English
Published: Universidad Nacional de Colombia 2003-01-01
Series:Acta Biológica Colombiana
Subjects:
Ovocito
Xenopus Laevis
Voltaje Clamp
KV1.1
Shaker
Expresiónheteróloga
Online Access:https://revistas.unal.edu.co/index.php/actabiol/article/view/26111
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spelling doaj-2a06df01605641d1876afc04092c422a2020-11-25T01:05:14ZengUniversidad Nacional de ColombiaActa Biológica Colombiana0120-548X1900-16492003-01-0181596723207Voltage gated potassium channels expressed in Xenopus laevis(AMPHIBIA) oocytesHedna Chaves0Carlos Villalba1Luisa Lagos2Rafael Vargas3Francisco Martínez-Wittinghan4Carlos Clavijo5Marcela Camacho6Universidad de Los AndesUniversidad Nacional de ColombiaUniversidad Nacional de ColombiaUniversidad Nacional de ColombiaCentro Internacional de FísicaUniversidad Nacional de ColombiaUniversidad Nacional de ColombiaHeterologous expression has been an important tool for structural and functionalcharacterization of proteins. The study of biophysical properties of ion channels,pumps and transporters has been possible thanks to their expression in Xenopuslaevisoocytes. Here we report the expression of two voltage gated channels, Kv1.1and Shaker, in X. laevisoocytes using a method for oocyte extraction, isolation, cul-ture, and microinjection adapted to the latitude and altitude conditions of Bogotá,Colombia.https://revistas.unal.edu.co/index.php/actabiol/article/view/26111OvocitoXenopus LaevisVoltaje ClampKV1.1ShakerExpresiónheteróloga
collection DOAJ
language English
format Article
sources DOAJ
author Hedna Chaves
Carlos Villalba
Luisa Lagos
Rafael Vargas
Francisco Martínez-Wittinghan
Carlos Clavijo
Marcela Camacho
spellingShingle Hedna Chaves
Carlos Villalba
Luisa Lagos
Rafael Vargas
Francisco Martínez-Wittinghan
Carlos Clavijo
Marcela Camacho
Voltage gated potassium channels expressed in Xenopus laevis(AMPHIBIA) oocytes
Acta Biológica Colombiana
Ovocito
Xenopus Laevis
Voltaje Clamp
KV1.1
Shaker
Expresiónheteróloga
author_facet Hedna Chaves
Carlos Villalba
Luisa Lagos
Rafael Vargas
Francisco Martínez-Wittinghan
Carlos Clavijo
Marcela Camacho
author_sort Hedna Chaves
title Voltage gated potassium channels expressed in Xenopus laevis(AMPHIBIA) oocytes
title_short Voltage gated potassium channels expressed in Xenopus laevis(AMPHIBIA) oocytes
title_full Voltage gated potassium channels expressed in Xenopus laevis(AMPHIBIA) oocytes
title_fullStr Voltage gated potassium channels expressed in Xenopus laevis(AMPHIBIA) oocytes
title_full_unstemmed Voltage gated potassium channels expressed in Xenopus laevis(AMPHIBIA) oocytes
title_sort voltage gated potassium channels expressed in xenopus laevis(amphibia) oocytes
publisher Universidad Nacional de Colombia
series Acta Biológica Colombiana
issn 0120-548X
1900-1649
publishDate 2003-01-01
description Heterologous expression has been an important tool for structural and functionalcharacterization of proteins. The study of biophysical properties of ion channels,pumps and transporters has been possible thanks to their expression in Xenopuslaevisoocytes. Here we report the expression of two voltage gated channels, Kv1.1and Shaker, in X. laevisoocytes using a method for oocyte extraction, isolation, cul-ture, and microinjection adapted to the latitude and altitude conditions of Bogotá,Colombia.
topic Ovocito
Xenopus Laevis
Voltaje Clamp
KV1.1
Shaker
Expresiónheteróloga
url https://revistas.unal.edu.co/index.php/actabiol/article/view/26111
work_keys_str_mv AT hednachaves voltagegatedpotassiumchannelsexpressedinxenopuslaevisamphibiaoocytes
AT carlosvillalba voltagegatedpotassiumchannelsexpressedinxenopuslaevisamphibiaoocytes
AT luisalagos voltagegatedpotassiumchannelsexpressedinxenopuslaevisamphibiaoocytes
AT rafaelvargas voltagegatedpotassiumchannelsexpressedinxenopuslaevisamphibiaoocytes
AT franciscomartinezwittinghan voltagegatedpotassiumchannelsexpressedinxenopuslaevisamphibiaoocytes
AT carlosclavijo voltagegatedpotassiumchannelsexpressedinxenopuslaevisamphibiaoocytes
AT marcelacamacho voltagegatedpotassiumchannelsexpressedinxenopuslaevisamphibiaoocytes
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