In vitro anti-diabetic, anti-obesity and antioxidant proprieties of Juniperus phoenicea L. leaves from Tunisia
Objective: To examine chemical composition and antioxidant activity as well as the in vitro α-amylase and pancreatic lipase inhibitory activities of the essential oil and various extracts of Juniperus phoenicea (J. phoenicea). Methods: Essential oil obtained by steam distillation were analyzed by ga...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Wolters Kluwer Medknow Publications
2014-07-01
|
Series: | Asian Pacific Journal of Tropical Biomedicine |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2221169115300654 |
id |
doaj-2a0a396e7a69499cb718d53ff34a6258 |
---|---|
record_format |
Article |
spelling |
doaj-2a0a396e7a69499cb718d53ff34a62582020-11-24T23:14:29ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Tropical Biomedicine2221-16912014-07-014S2S649S65510.12980/APJTB.4.201414B114In vitro anti-diabetic, anti-obesity and antioxidant proprieties of Juniperus phoenicea L. leaves from TunisiaHenda Keskes0Kais Mnafgui1Khaled Hamden2Mohamed Damak3Abdelfattah El Feki4Noureddine Allouche5Laboratory of Chemistry of Natural Products, University of Sfax, Faculty of Sciences of Sfax, BP “1171”, 3000, Sfax, TunisiaLaboratory of Chemistry of Natural Products, University of Sfax, Faculty of Sciences of Sfax, BP “1171”, 3000, Sfax, TunisiaLaboratory of Animal Ecophysiology, University of Sfax, Faculty of Sciences of Sfax, BP ”1171”, 3000, Sfax, TunisiaLaboratory of Chemistry of Natural Products, University of Sfax, Faculty of Sciences of Sfax, BP “1171”, 3000, Sfax, TunisiaLaboratory of Animal Ecophysiology, University of Sfax, Faculty of Sciences of Sfax, BP ”1171”, 3000, Sfax, TunisiaLaboratory of Chemistry of Natural Products, University of Sfax, Faculty of Sciences of Sfax, BP “1171”, 3000, Sfax, TunisiaObjective: To examine chemical composition and antioxidant activity as well as the in vitro α-amylase and pancreatic lipase inhibitory activities of the essential oil and various extracts of Juniperus phoenicea (J. phoenicea). Methods: Essential oil obtained by steam distillation were analyzed by gas chromatography-mass spectrometry technique. The antioxidant activity of the essential oil and various extracts of J. phoenicea were determined by DPPH and β-carotene bleaching methods. Results: Gas chromatography-mass spectrometry analysis of the J. phoenicea essential oil resulted in the identification of 37 compounds, representing 96.98% of the oil; α-Pinene (24.02%), limonene (7.94%), D-3-Carene (16.9%), Germacrene D (11.98%), Germacrene B (5.40%) and δ-cadinene (6.52%) were the major compounds. The IC50 values of essential oil, hexane and methanol extracts against α-amylase were 35.44, 30.15 and 53.76 μg/mL respectively, and those against pancreatic lipase were 66.15, 68.47 and 60.22 μg/mL respectively, suggesting powerful anti-diabetic and anti-obesity effects. Antioxidant activity (IC50=2 μg/mL) and total phenolics content (265 mg as gallic acid equivalent/g extract) of the methanol extract were found to be the highest compared to the other extracts. Conclusions: The findings showed that the extents of α-amylase and pancreatic lipase inhibitory activities of the J. phoenicea extracts as well as their antioxidant activity are in accordance with total phenolics contents. Leaves of J. phoenicea being rich in phenolics may provide a good source of natural products with interesting medicinal properties.http://www.sciencedirect.com/science/article/pii/S2221169115300654Chemical compositionα-AmylaseLipaseEssential oilAntioxidant activity |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Henda Keskes Kais Mnafgui Khaled Hamden Mohamed Damak Abdelfattah El Feki Noureddine Allouche |
spellingShingle |
Henda Keskes Kais Mnafgui Khaled Hamden Mohamed Damak Abdelfattah El Feki Noureddine Allouche In vitro anti-diabetic, anti-obesity and antioxidant proprieties of Juniperus phoenicea L. leaves from Tunisia Asian Pacific Journal of Tropical Biomedicine Chemical composition α-Amylase Lipase Essential oil Antioxidant activity |
author_facet |
Henda Keskes Kais Mnafgui Khaled Hamden Mohamed Damak Abdelfattah El Feki Noureddine Allouche |
author_sort |
Henda Keskes |
title |
In vitro anti-diabetic, anti-obesity and antioxidant proprieties of Juniperus phoenicea L. leaves from Tunisia |
title_short |
In vitro anti-diabetic, anti-obesity and antioxidant proprieties of Juniperus phoenicea L. leaves from Tunisia |
title_full |
In vitro anti-diabetic, anti-obesity and antioxidant proprieties of Juniperus phoenicea L. leaves from Tunisia |
title_fullStr |
In vitro anti-diabetic, anti-obesity and antioxidant proprieties of Juniperus phoenicea L. leaves from Tunisia |
title_full_unstemmed |
In vitro anti-diabetic, anti-obesity and antioxidant proprieties of Juniperus phoenicea L. leaves from Tunisia |
title_sort |
in vitro anti-diabetic, anti-obesity and antioxidant proprieties of juniperus phoenicea l. leaves from tunisia |
publisher |
Wolters Kluwer Medknow Publications |
series |
Asian Pacific Journal of Tropical Biomedicine |
issn |
2221-1691 |
publishDate |
2014-07-01 |
description |
Objective: To examine chemical composition and antioxidant activity as well as the in vitro α-amylase and pancreatic lipase inhibitory activities of the essential oil and various extracts of Juniperus phoenicea (J. phoenicea).
Methods: Essential oil obtained by steam distillation were analyzed by gas chromatography-mass spectrometry technique. The antioxidant activity of the essential oil and various extracts of J. phoenicea were determined by DPPH and β-carotene bleaching methods.
Results: Gas chromatography-mass spectrometry analysis of the J. phoenicea essential oil resulted in the identification of 37 compounds, representing 96.98% of the oil; α-Pinene (24.02%), limonene (7.94%), D-3-Carene (16.9%), Germacrene D (11.98%), Germacrene B (5.40%) and δ-cadinene (6.52%) were the major compounds. The IC50 values of essential oil, hexane and methanol extracts against α-amylase were 35.44, 30.15 and 53.76 μg/mL respectively, and those against pancreatic lipase were 66.15, 68.47 and 60.22 μg/mL respectively, suggesting powerful anti-diabetic and anti-obesity effects. Antioxidant activity (IC50=2 μg/mL) and total phenolics content (265 mg as gallic acid equivalent/g extract) of the methanol extract were found to be the highest compared to the other extracts.
Conclusions: The findings showed that the extents of α-amylase and pancreatic lipase inhibitory activities of the J. phoenicea extracts as well as their antioxidant activity are in accordance with total phenolics contents. Leaves of J. phoenicea being rich in phenolics may provide a good source of natural products with interesting medicinal properties. |
topic |
Chemical composition α-Amylase Lipase Essential oil Antioxidant activity |
url |
http://www.sciencedirect.com/science/article/pii/S2221169115300654 |
work_keys_str_mv |
AT hendakeskes invitroantidiabeticantiobesityandantioxidantproprietiesofjuniperusphoenicealleavesfromtunisia AT kaismnafgui invitroantidiabeticantiobesityandantioxidantproprietiesofjuniperusphoenicealleavesfromtunisia AT khaledhamden invitroantidiabeticantiobesityandantioxidantproprietiesofjuniperusphoenicealleavesfromtunisia AT mohameddamak invitroantidiabeticantiobesityandantioxidantproprietiesofjuniperusphoenicealleavesfromtunisia AT abdelfattahelfeki invitroantidiabeticantiobesityandantioxidantproprietiesofjuniperusphoenicealleavesfromtunisia AT noureddineallouche invitroantidiabeticantiobesityandantioxidantproprietiesofjuniperusphoenicealleavesfromtunisia |
_version_ |
1725594107745665024 |