Global Reprogramming of Transcription in Chinese Fir (Cunninghamia lanceolata) during Progressive Drought Stress and after Rewatering
Chinese fir (Cunninghamia lanceolata), an evergreen conifer, is the most commonly grown afforestation species in southeast China due to its rapid growth and good wood qualities. To gain a better understanding of the drought-signalling pathway and the molecular metabolic reactions involved in the dro...
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doaj-2a10c65d88734b8f9514253a6901469d2020-11-24T22:06:43ZengMDPI AGInternational Journal of Molecular Sciences1422-00672015-07-01167151941521910.3390/ijms160715194ijms160715194Global Reprogramming of Transcription in Chinese Fir (Cunninghamia lanceolata) during Progressive Drought Stress and after RewateringRuiyang Hu0Bo Wu1Huiquan Zheng2Dehuo Hu3Xinjie Wang4Hongjing Duan5Yuhan Sun6Jinxing Wang7Yue Zhang8Yun Li9National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, ChinaNational Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, ChinaGuangdong Academy of Forestry, Guangzhou 510520, ChinaGuangdong Academy of Forestry, Guangzhou 510520, ChinaCollege of Forestry, Beijing Forestry University, Beijing 100083, ChinaNational Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, ChinaNational Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, ChinaNational Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, ChinaNational Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, ChinaNational Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, ChinaChinese fir (Cunninghamia lanceolata), an evergreen conifer, is the most commonly grown afforestation species in southeast China due to its rapid growth and good wood qualities. To gain a better understanding of the drought-signalling pathway and the molecular metabolic reactions involved in the drought response, we performed a genome-wide transcription analysis using RNA sequence data. In this study, Chinese fir plantlets were subjected to progressively prolonged drought stress, up to 15 d, followed by rewatering under controlled environmental conditions. Based on observed morphological changes, plantlets experienced mild, moderate, or severe water stress before rehydration. Transcriptome analysis of plantlets, representing control and mild, moderate, and severe drought-stress treatments, and the rewatered plantlets, identified several thousand genes whose expression was altered in response to drought stress. Many genes whose expression was tightly coupled to the levels of drought stress were identified, suggesting involvement in Chinese fir drought adaptation responses. These genes were associated with transcription factors, signal transport, stress kinases, phytohormone signalling, and defence/stress response. The present study provides the most comprehensive transcriptome resource and the first dynamic transcriptome profiles of Chinese fir under drought stress. The drought-responsive genes identified in this study could provide further information for understanding the mechanisms of drought tolerance in Chinese fir.http://www.mdpi.com/1422-0067/16/7/15194Cunninghamia lanceolatadrought stresstranscriptomegene expressionrewatering |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ruiyang Hu Bo Wu Huiquan Zheng Dehuo Hu Xinjie Wang Hongjing Duan Yuhan Sun Jinxing Wang Yue Zhang Yun Li |
spellingShingle |
Ruiyang Hu Bo Wu Huiquan Zheng Dehuo Hu Xinjie Wang Hongjing Duan Yuhan Sun Jinxing Wang Yue Zhang Yun Li Global Reprogramming of Transcription in Chinese Fir (Cunninghamia lanceolata) during Progressive Drought Stress and after Rewatering International Journal of Molecular Sciences Cunninghamia lanceolata drought stress transcriptome gene expression rewatering |
author_facet |
Ruiyang Hu Bo Wu Huiquan Zheng Dehuo Hu Xinjie Wang Hongjing Duan Yuhan Sun Jinxing Wang Yue Zhang Yun Li |
author_sort |
Ruiyang Hu |
title |
Global Reprogramming of Transcription in Chinese Fir (Cunninghamia lanceolata) during Progressive Drought Stress and after Rewatering |
title_short |
Global Reprogramming of Transcription in Chinese Fir (Cunninghamia lanceolata) during Progressive Drought Stress and after Rewatering |
title_full |
Global Reprogramming of Transcription in Chinese Fir (Cunninghamia lanceolata) during Progressive Drought Stress and after Rewatering |
title_fullStr |
Global Reprogramming of Transcription in Chinese Fir (Cunninghamia lanceolata) during Progressive Drought Stress and after Rewatering |
title_full_unstemmed |
Global Reprogramming of Transcription in Chinese Fir (Cunninghamia lanceolata) during Progressive Drought Stress and after Rewatering |
title_sort |
global reprogramming of transcription in chinese fir (cunninghamia lanceolata) during progressive drought stress and after rewatering |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2015-07-01 |
description |
Chinese fir (Cunninghamia lanceolata), an evergreen conifer, is the most commonly grown afforestation species in southeast China due to its rapid growth and good wood qualities. To gain a better understanding of the drought-signalling pathway and the molecular metabolic reactions involved in the drought response, we performed a genome-wide transcription analysis using RNA sequence data. In this study, Chinese fir plantlets were subjected to progressively prolonged drought stress, up to 15 d, followed by rewatering under controlled environmental conditions. Based on observed morphological changes, plantlets experienced mild, moderate, or severe water stress before rehydration. Transcriptome analysis of plantlets, representing control and mild, moderate, and severe drought-stress treatments, and the rewatered plantlets, identified several thousand genes whose expression was altered in response to drought stress. Many genes whose expression was tightly coupled to the levels of drought stress were identified, suggesting involvement in Chinese fir drought adaptation responses. These genes were associated with transcription factors, signal transport, stress kinases, phytohormone signalling, and defence/stress response. The present study provides the most comprehensive transcriptome resource and the first dynamic transcriptome profiles of Chinese fir under drought stress. The drought-responsive genes identified in this study could provide further information for understanding the mechanisms of drought tolerance in Chinese fir. |
topic |
Cunninghamia lanceolata drought stress transcriptome gene expression rewatering |
url |
http://www.mdpi.com/1422-0067/16/7/15194 |
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