Modules for in vitro metabolic engineering: Pathway assembly for bio-based production of value-added chemicals

• Main Authors: Hironori Taniguchi, Kenji Okano, Kohsuke Honda
• Format: Article
• Language: English
• Published: KeAi Communications Co., Ltd. 2017-06-01
• Series: Synthetic and Systems Biotechnology
• Subjects: In vitro metabolic engineering; Module; Glycolysis; Pentose phosphate pathway; Cofactor management
• Online Access: http://www.sciencedirect.com/science/article/pii/S2405805X17300182

Bio-based chemical production has drawn attention regarding the realization of a sustainable society. In vitro metabolic engineering is one of the methods used for the bio-based production of value-added chemicals. This method involves the reconstitution of natural or artificial metabolic pathways by assembling purified/semi-purified enzymes in vitro. Enzymes from distinct sources can be combined to construct desired reaction cascades with fewer biological constraints in one vessel, enabling easier pathway design with high modularity. Multiple modules have been designed, built, tested, and improved by different groups for different purpose. In this review, we focus on these in vitro metabolic engineering modules, especially focusing on the carbon metabolism, and present an overview of input modules, output modules, and other modules related to cofactor management.