The Drosophila microtubule-associated protein mars stabilizes mitotic spindles by crosslinking microtubules through its N-terminal region.
Correct segregation of genetic material relies on proper assembly and maintenance of the mitotic spindle. How the highly dynamic microtubules (MTs) are maintained in stable mitotic spindles is a key question to be answered. Motor and non-motor microtubule associated proteins (MAPs) have been reporte...
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2013-01-01
|
Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC3617137?pdf=render |
id |
doaj-2a257b4ad70e469392394b53e3339eb4 |
---|---|
record_format |
Article |
spelling |
doaj-2a257b4ad70e469392394b53e3339eb42020-11-25T02:15:31ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0184e6059610.1371/journal.pone.0060596The Drosophila microtubule-associated protein mars stabilizes mitotic spindles by crosslinking microtubules through its N-terminal region.Gang ZhangHamze BeatiJakob NilssonAndreas WodarzCorrect segregation of genetic material relies on proper assembly and maintenance of the mitotic spindle. How the highly dynamic microtubules (MTs) are maintained in stable mitotic spindles is a key question to be answered. Motor and non-motor microtubule associated proteins (MAPs) have been reported to stabilize the dynamic spindle through crosslinking adjacent MTs. Mars, a novel MAP, is essential for the early development of Drosophila embryos. Previous studies showed that Mars is required for maintaining an intact mitotic spindle but did not provide a molecular mechanism for this function. Here we show that Mars is able to stabilize the mitotic spindle in vivo. Both in vivo and in vitro data reveal that the N-terminal region of Mars functions in the stabilization of the mitotic spindle by crosslinking adjacent MTs.http://europepmc.org/articles/PMC3617137?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Gang Zhang Hamze Beati Jakob Nilsson Andreas Wodarz |
spellingShingle |
Gang Zhang Hamze Beati Jakob Nilsson Andreas Wodarz The Drosophila microtubule-associated protein mars stabilizes mitotic spindles by crosslinking microtubules through its N-terminal region. PLoS ONE |
author_facet |
Gang Zhang Hamze Beati Jakob Nilsson Andreas Wodarz |
author_sort |
Gang Zhang |
title |
The Drosophila microtubule-associated protein mars stabilizes mitotic spindles by crosslinking microtubules through its N-terminal region. |
title_short |
The Drosophila microtubule-associated protein mars stabilizes mitotic spindles by crosslinking microtubules through its N-terminal region. |
title_full |
The Drosophila microtubule-associated protein mars stabilizes mitotic spindles by crosslinking microtubules through its N-terminal region. |
title_fullStr |
The Drosophila microtubule-associated protein mars stabilizes mitotic spindles by crosslinking microtubules through its N-terminal region. |
title_full_unstemmed |
The Drosophila microtubule-associated protein mars stabilizes mitotic spindles by crosslinking microtubules through its N-terminal region. |
title_sort |
drosophila microtubule-associated protein mars stabilizes mitotic spindles by crosslinking microtubules through its n-terminal region. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2013-01-01 |
description |
Correct segregation of genetic material relies on proper assembly and maintenance of the mitotic spindle. How the highly dynamic microtubules (MTs) are maintained in stable mitotic spindles is a key question to be answered. Motor and non-motor microtubule associated proteins (MAPs) have been reported to stabilize the dynamic spindle through crosslinking adjacent MTs. Mars, a novel MAP, is essential for the early development of Drosophila embryos. Previous studies showed that Mars is required for maintaining an intact mitotic spindle but did not provide a molecular mechanism for this function. Here we show that Mars is able to stabilize the mitotic spindle in vivo. Both in vivo and in vitro data reveal that the N-terminal region of Mars functions in the stabilization of the mitotic spindle by crosslinking adjacent MTs. |
url |
http://europepmc.org/articles/PMC3617137?pdf=render |
work_keys_str_mv |
AT gangzhang thedrosophilamicrotubuleassociatedproteinmarsstabilizesmitoticspindlesbycrosslinkingmicrotubulesthroughitsnterminalregion AT hamzebeati thedrosophilamicrotubuleassociatedproteinmarsstabilizesmitoticspindlesbycrosslinkingmicrotubulesthroughitsnterminalregion AT jakobnilsson thedrosophilamicrotubuleassociatedproteinmarsstabilizesmitoticspindlesbycrosslinkingmicrotubulesthroughitsnterminalregion AT andreaswodarz thedrosophilamicrotubuleassociatedproteinmarsstabilizesmitoticspindlesbycrosslinkingmicrotubulesthroughitsnterminalregion AT gangzhang drosophilamicrotubuleassociatedproteinmarsstabilizesmitoticspindlesbycrosslinkingmicrotubulesthroughitsnterminalregion AT hamzebeati drosophilamicrotubuleassociatedproteinmarsstabilizesmitoticspindlesbycrosslinkingmicrotubulesthroughitsnterminalregion AT jakobnilsson drosophilamicrotubuleassociatedproteinmarsstabilizesmitoticspindlesbycrosslinkingmicrotubulesthroughitsnterminalregion AT andreaswodarz drosophilamicrotubuleassociatedproteinmarsstabilizesmitoticspindlesbycrosslinkingmicrotubulesthroughitsnterminalregion |
_version_ |
1724895769934168064 |