Isolation and Structural Characterization of Bioactive Molecules on Prostate Cancer from Mayan Traditional Medicinal Plants
Prostate cancer is the most common cancer in men around the world. It is a complex and heterogeneous disease in which androgens and their receptors play a crucial role in the progression and development. The current treatment for prostate cancer is a combination of surgery, hormone therapy, radiatio...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2018-08-01
|
Series: | Pharmaceuticals |
Subjects: | |
Online Access: | http://www.mdpi.com/1424-8247/11/3/78 |
id |
doaj-2a405b36ff9044ef82ccfdbfcff6f80e |
---|---|
record_format |
Article |
spelling |
doaj-2a405b36ff9044ef82ccfdbfcff6f80e2020-11-25T03:24:51ZengMDPI AGPharmaceuticals1424-82472018-08-011137810.3390/ph11030078ph11030078Isolation and Structural Characterization of Bioactive Molecules on Prostate Cancer from Mayan Traditional Medicinal PlantsRafael Sebastián Fort0Juan M. Trinidad Barnech1Juliette Dourron2Marcos Colazzo3Francisco J. Aguirre-Crespo4María Ana Duhagon5Guzmán Álvarez6Laboratorio de Interacciones Moleculares, Facultad de Ciencias, Universidad de la República, Montevideo, C.P. 11400, UruguayLaboratorio de Interacciones Moleculares, Facultad de Ciencias, Universidad de la República, Montevideo, C.P. 11400, UruguayLaboratorio de Moléculas Bioactivas, CENUR Litoral Norte, Universidad de la República, Ruta 3 (km 363), Paysandú, C.P. 60000, UruguayDepartamento de Química del Litoral, CENUR Litoral Norte, Universidad de la República, Paysandú, C.P. 60000, UruguayFacultad de Ciencias Químico Biológicas, Universidad Autónoma de Campeche, Campeche, C.P. 24039, MexicoLaboratorio de Interacciones Moleculares, Facultad de Ciencias, Universidad de la República, Montevideo, C.P. 11400, UruguayLaboratorio de Moléculas Bioactivas, CENUR Litoral Norte, Universidad de la República, Ruta 3 (km 363), Paysandú, C.P. 60000, UruguayProstate cancer is the most common cancer in men around the world. It is a complex and heterogeneous disease in which androgens and their receptors play a crucial role in the progression and development. The current treatment for prostate cancer is a combination of surgery, hormone therapy, radiation and chemotherapy. Therapeutic agents commonly used in the clinic include steroidal and non-steroidal anti-androgens, such as cyproterone acetate, bicalutamide and enzalutamide. These few agents have multiple adverse effects and are not 100% effective. Several plant compounds and mixtures, including grape seed polyphenol extracts, lycopene and tomato preparations, soy isoflavones, and green tea extracts, have been shown to be effective against prostate cancer cell growth. In vivo activity of some isolated compounds like capsaicin and curcumin was reported in prostate cancer murine models. We prepared a library of plant extracts from traditional Mayan medicine. These plants were selected for their use in the contemporaneous Mayan communities for the treatment of different diseases. The extracts were assessed in a phenotypic screening using LNCaP prostate cancer androgen sensitive cell line, with a fixed dose of 25 μg/mL. MTT assay identified seven out of ten plants with interesting anti-neoplastic activity. Extracts from these plants were subjected to a bioguided fractionation to study their major components. We identified three compounds with anti-neoplastic effects against LNCaP cells, one of which shows selectivity for neoplastic compared to benign cells.http://www.mdpi.com/1424-8247/11/3/78prostate cancerin vitroLNCaPnatural productplantsMayan medicine |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Rafael Sebastián Fort Juan M. Trinidad Barnech Juliette Dourron Marcos Colazzo Francisco J. Aguirre-Crespo María Ana Duhagon Guzmán Álvarez |
spellingShingle |
Rafael Sebastián Fort Juan M. Trinidad Barnech Juliette Dourron Marcos Colazzo Francisco J. Aguirre-Crespo María Ana Duhagon Guzmán Álvarez Isolation and Structural Characterization of Bioactive Molecules on Prostate Cancer from Mayan Traditional Medicinal Plants Pharmaceuticals prostate cancer in vitro LNCaP natural product plants Mayan medicine |
author_facet |
Rafael Sebastián Fort Juan M. Trinidad Barnech Juliette Dourron Marcos Colazzo Francisco J. Aguirre-Crespo María Ana Duhagon Guzmán Álvarez |
author_sort |
Rafael Sebastián Fort |
title |
Isolation and Structural Characterization of Bioactive Molecules on Prostate Cancer from Mayan Traditional Medicinal Plants |
title_short |
Isolation and Structural Characterization of Bioactive Molecules on Prostate Cancer from Mayan Traditional Medicinal Plants |
title_full |
Isolation and Structural Characterization of Bioactive Molecules on Prostate Cancer from Mayan Traditional Medicinal Plants |
title_fullStr |
Isolation and Structural Characterization of Bioactive Molecules on Prostate Cancer from Mayan Traditional Medicinal Plants |
title_full_unstemmed |
Isolation and Structural Characterization of Bioactive Molecules on Prostate Cancer from Mayan Traditional Medicinal Plants |
title_sort |
isolation and structural characterization of bioactive molecules on prostate cancer from mayan traditional medicinal plants |
publisher |
MDPI AG |
series |
Pharmaceuticals |
issn |
1424-8247 |
publishDate |
2018-08-01 |
description |
Prostate cancer is the most common cancer in men around the world. It is a complex and heterogeneous disease in which androgens and their receptors play a crucial role in the progression and development. The current treatment for prostate cancer is a combination of surgery, hormone therapy, radiation and chemotherapy. Therapeutic agents commonly used in the clinic include steroidal and non-steroidal anti-androgens, such as cyproterone acetate, bicalutamide and enzalutamide. These few agents have multiple adverse effects and are not 100% effective. Several plant compounds and mixtures, including grape seed polyphenol extracts, lycopene and tomato preparations, soy isoflavones, and green tea extracts, have been shown to be effective against prostate cancer cell growth. In vivo activity of some isolated compounds like capsaicin and curcumin was reported in prostate cancer murine models. We prepared a library of plant extracts from traditional Mayan medicine. These plants were selected for their use in the contemporaneous Mayan communities for the treatment of different diseases. The extracts were assessed in a phenotypic screening using LNCaP prostate cancer androgen sensitive cell line, with a fixed dose of 25 μg/mL. MTT assay identified seven out of ten plants with interesting anti-neoplastic activity. Extracts from these plants were subjected to a bioguided fractionation to study their major components. We identified three compounds with anti-neoplastic effects against LNCaP cells, one of which shows selectivity for neoplastic compared to benign cells. |
topic |
prostate cancer in vitro LNCaP natural product plants Mayan medicine |
url |
http://www.mdpi.com/1424-8247/11/3/78 |
work_keys_str_mv |
AT rafaelsebastianfort isolationandstructuralcharacterizationofbioactivemoleculesonprostatecancerfrommayantraditionalmedicinalplants AT juanmtrinidadbarnech isolationandstructuralcharacterizationofbioactivemoleculesonprostatecancerfrommayantraditionalmedicinalplants AT juliettedourron isolationandstructuralcharacterizationofbioactivemoleculesonprostatecancerfrommayantraditionalmedicinalplants AT marcoscolazzo isolationandstructuralcharacterizationofbioactivemoleculesonprostatecancerfrommayantraditionalmedicinalplants AT franciscojaguirrecrespo isolationandstructuralcharacterizationofbioactivemoleculesonprostatecancerfrommayantraditionalmedicinalplants AT mariaanaduhagon isolationandstructuralcharacterizationofbioactivemoleculesonprostatecancerfrommayantraditionalmedicinalplants AT guzmanalvarez isolationandstructuralcharacterizationofbioactivemoleculesonprostatecancerfrommayantraditionalmedicinalplants |
_version_ |
1724599406256193536 |