In vivo detection of optically-evoked opioid peptide release

Though the last decade has seen accelerated advances in techniques and technologies to perturb neuronal circuitry in the brain, we are still poorly equipped to adequately dissect endogenous peptide release in vivo. To this end we developed a system that combines in vivo optogenetics with microdialys...

Full description

Bibliographic Details
Main Authors:	Ream Al-Hasani, Jenny-Marie T Wong, Omar S Mabrouk, Jordan G McCall, Gavin P Schmitz, Kirsten A Porter-Stransky, Brandon J Aragona, Robert T Kennedy, Michael R Bruchas
Format:	Article
Language:	English
Published:	eLife Sciences Publications Ltd 2018-09-01
Series:	eLife
Subjects:	opioids peptides optogenetics nucleus accembens mass spectrometry microdialysis
Online Access:	https://elifesciences.org/articles/36520

id	doaj-2a45ab788e4546b0b17f41431d154705
record_format	Article
spelling	doaj-2a45ab788e4546b0b17f41431d1547052021-05-05T16:07:44ZengeLife Sciences Publications LtdeLife2050-084X2018-09-01710.7554/eLife.36520In vivo detection of optically-evoked opioid peptide releaseReam Al-Hasani0https://orcid.org/0000-0002-8781-6234Jenny-Marie T Wong1Omar S Mabrouk2Jordan G McCall3https://orcid.org/0000-0001-8295-0664Gavin P Schmitz4Kirsten A Porter-Stransky5Brandon J Aragona6Robert T Kennedy7Michael R Bruchas8https://orcid.org/0000-0003-4713-7816Department of Anesthesiology, Division of Basic Research, Washington University School of Medicine, St. Louis, United States; Department of Pharmaceutical and Administrative Sciences, St. Louis College of Pharmacy, St. Louis, United States; Center for Clinical Pharmacology, Washington University School of Medicine and St. Louis College of Pharmacy, St. Louis, United StatesDepartment of Chemistry, University of Michigan, Ann Arbor, United StatesDepartment of Chemistry, University of Michigan, Ann Arbor, United States; Department of Pharmacology, University of Michigan, Ann Arbor, United StatesDepartment of Anesthesiology, Division of Basic Research, Washington University School of Medicine, St. Louis, United States; Department of Pharmaceutical and Administrative Sciences, St. Louis College of Pharmacy, St. Louis, United States; Center for Clinical Pharmacology, Washington University School of Medicine and St. Louis College of Pharmacy, St. Louis, United States; Washington University Pain Center, Washington University School of Medicine, St. Louis, United StatesDepartment of Anesthesiology, Division of Basic Research, Washington University School of Medicine, St. Louis, United States; Center for Clinical Pharmacology, Washington University School of Medicine and St. Louis College of Pharmacy, St. Louis, United StatesDepartment of Psychology, University of Michigan, Ann Arbor, United StatesDepartment of Psychology, University of Michigan, Ann Arbor, United StatesDepartment of Chemistry, University of Michigan, Ann Arbor, United States; Department of Pharmacology, University of Michigan, Ann Arbor, United StatesDepartment of Anesthesiology, Division of Basic Research, Washington University School of Medicine, St. Louis, United States; Washington University Pain Center, Washington University School of Medicine, St. Louis, United States; Department of Neuroscience, Washington University School of Medicine, St. Louis, United States; Department of Anesthesiology and Pain Medicine, Center for the Neurobiology of Addiction, Pain, and Emotion, University of Washington, Washington, United StatesThough the last decade has seen accelerated advances in techniques and technologies to perturb neuronal circuitry in the brain, we are still poorly equipped to adequately dissect endogenous peptide release in vivo. To this end we developed a system that combines in vivo optogenetics with microdialysis and a highly sensitive mass spectrometry-based assay to measure opioid peptide release in freely moving rodents.https://elifesciences.org/articles/36520opioids peptidesoptogeneticsnucleus accembensmass spectrometrymicrodialysis
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Ream Al-Hasani Jenny-Marie T Wong Omar S Mabrouk Jordan G McCall Gavin P Schmitz Kirsten A Porter-Stransky Brandon J Aragona Robert T Kennedy Michael R Bruchas
spellingShingle	Ream Al-Hasani Jenny-Marie T Wong Omar S Mabrouk Jordan G McCall Gavin P Schmitz Kirsten A Porter-Stransky Brandon J Aragona Robert T Kennedy Michael R Bruchas In vivo detection of optically-evoked opioid peptide release eLife opioids peptides optogenetics nucleus accembens mass spectrometry microdialysis
author_facet	Ream Al-Hasani Jenny-Marie T Wong Omar S Mabrouk Jordan G McCall Gavin P Schmitz Kirsten A Porter-Stransky Brandon J Aragona Robert T Kennedy Michael R Bruchas
author_sort	Ream Al-Hasani
title	In vivo detection of optically-evoked opioid peptide release
title_short	In vivo detection of optically-evoked opioid peptide release
title_full	In vivo detection of optically-evoked opioid peptide release
title_fullStr	In vivo detection of optically-evoked opioid peptide release
title_full_unstemmed	In vivo detection of optically-evoked opioid peptide release
title_sort	in vivo detection of optically-evoked opioid peptide release
publisher	eLife Sciences Publications Ltd
series	eLife
issn	2050-084X
publishDate	2018-09-01
description	Though the last decade has seen accelerated advances in techniques and technologies to perturb neuronal circuitry in the brain, we are still poorly equipped to adequately dissect endogenous peptide release in vivo. To this end we developed a system that combines in vivo optogenetics with microdialysis and a highly sensitive mass spectrometry-based assay to measure opioid peptide release in freely moving rodents.
topic	opioids peptides optogenetics nucleus accembens mass spectrometry microdialysis
url	https://elifesciences.org/articles/36520
work_keys_str_mv	AT reamalhasani invivodetectionofopticallyevokedopioidpeptiderelease AT jennymarietwong invivodetectionofopticallyevokedopioidpeptiderelease AT omarsmabrouk invivodetectionofopticallyevokedopioidpeptiderelease AT jordangmccall invivodetectionofopticallyevokedopioidpeptiderelease AT gavinpschmitz invivodetectionofopticallyevokedopioidpeptiderelease AT kirstenaporterstransky invivodetectionofopticallyevokedopioidpeptiderelease AT brandonjaragona invivodetectionofopticallyevokedopioidpeptiderelease AT roberttkennedy invivodetectionofopticallyevokedopioidpeptiderelease AT michaelrbruchas invivodetectionofopticallyevokedopioidpeptiderelease
_version_	1721459529058615296

In vivo detection of optically-evoked opioid peptide release

Similar Items