Detailed Analysis of Apoptosis and Delayed Luminescence of Human Leukemia Jurkat T Cells after Proton Irradiation and Treatments with Oxidant Agents and Flavonoids
Following previous work, we investigated in more detail the relationship between apoptosis and delayed luminescence (DL) in human leukemia Jurkat T cells under a wide variety of treatments. We used menadione and hydrogen peroxide to induce oxidative stress and two flavonoids, quercetin, and epigallo...
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Online Access: | http://dx.doi.org/10.1155/2012/498914 |
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doaj-2a4a417f5bc9406cab05b77e01ff80972020-11-24T23:35:52ZengHindawi LimitedOxidative Medicine and Cellular Longevity1942-09001942-09942012-01-01201210.1155/2012/498914498914Detailed Analysis of Apoptosis and Delayed Luminescence of Human Leukemia Jurkat T Cells after Proton Irradiation and Treatments with Oxidant Agents and FlavonoidsIrina Baran0Constanta Ganea1Simona Privitera2Agata Scordino3Vincenza Barresi4Francesco Musumeci5Maria Magdalena Mocanu6Daniele F. Condorelli7Ioan Ursu8Rosaria Grasso9Marisa Gulino10Alexandru Garaiman11Nicolò Musso12Giuseppe A. Pablo Cirrone13Giacomo Cuttone14Department of Biophysics, “Carol Davila” University of Medicine and Pharmacy, 8 Eroii Sanitari, 050474 Bucharest, RomaniaDepartment of Biophysics, “Carol Davila” University of Medicine and Pharmacy, 8 Eroii Sanitari, 050474 Bucharest, RomaniaIstituto Nazionale di Fisica Nucleare, Laboratori Nazionali del Sud, via S. Sofia, 95125 Catania, ItalyIstituto Nazionale di Fisica Nucleare, Laboratori Nazionali del Sud, via S. Sofia, 95125 Catania, ItalySezione di Biochimica e Biologia Molecolare, Dipartimento di Scienze Chimiche, Università di Catania, 6 A. Doria, 95125 Catania, ItalyIstituto Nazionale di Fisica Nucleare, Laboratori Nazionali del Sud, via S. Sofia, 95125 Catania, ItalyDepartment of Biophysics, “Carol Davila” University of Medicine and Pharmacy, 8 Eroii Sanitari, 050474 Bucharest, RomaniaSezione di Biochimica e Biologia Molecolare, Dipartimento di Scienze Chimiche, Università di Catania, 6 A. Doria, 95125 Catania, Italy“Horia Hulubei” National Institute for Physics and Nuclear Engineering (IFIN-HH), 30 Reactorului, 077125 Bucharest, RomaniaIstituto Nazionale di Fisica Nucleare, Laboratori Nazionali del Sud, via S. Sofia, 95125 Catania, ItalyUniversità degli Studi di Enna “Kore”, Facoltà di Ingegneria, Architettura e delle Scienze Motorie, Cittadella Universitaria, 94100 Enna, ItalyDepartment of Biophysics, “Carol Davila” University of Medicine and Pharmacy, 8 Eroii Sanitari, 050474 Bucharest, RomaniaSezione di Biochimica e Biologia Molecolare, Dipartimento di Scienze Chimiche, Università di Catania, 6 A. Doria, 95125 Catania, ItalyIstituto Nazionale di Fisica Nucleare, Laboratori Nazionali del Sud, via S. Sofia, 95125 Catania, ItalyIstituto Nazionale di Fisica Nucleare, Laboratori Nazionali del Sud, via S. Sofia, 95125 Catania, ItalyFollowing previous work, we investigated in more detail the relationship between apoptosis and delayed luminescence (DL) in human leukemia Jurkat T cells under a wide variety of treatments. We used menadione and hydrogen peroxide to induce oxidative stress and two flavonoids, quercetin, and epigallocatechin gallate, applied alone or in combination with menadione or H2O2. 62 MeV proton beams were used to irradiate cells under a uniform dose of 2 or 10 Gy, respectively. We assessed apoptosis, cell cycle distributions, and DL. Menadione, H2O2 and quercetin were potent inducers of apoptosis and DL inhibitors. Quercetin decreased clonogenic survival and the NAD(P)H level in a dose-dependent manner. Proton irradiation with 2 Gy but not 10 Gy increased the apoptotic rate. However, both doses induced a substantial G2/M arrest. Quercetin reduced apoptosis and prolonged the G2/M arrest induced by radiation. DL spectroscopy indicated that proton irradiation disrupted the electron flow within Complex I of the mitochondrial respiratory chain, thus explaining the massive necrosis induced by 10 Gy of protons and also suggested an equivalent action of menadione and quercetin at the level of the Fe/S center N2, which may be mediated by their binding to a common site within Complex I, probably the rotenone-binding site.http://dx.doi.org/10.1155/2012/498914 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Irina Baran Constanta Ganea Simona Privitera Agata Scordino Vincenza Barresi Francesco Musumeci Maria Magdalena Mocanu Daniele F. Condorelli Ioan Ursu Rosaria Grasso Marisa Gulino Alexandru Garaiman Nicolò Musso Giuseppe A. Pablo Cirrone Giacomo Cuttone |
spellingShingle |
Irina Baran Constanta Ganea Simona Privitera Agata Scordino Vincenza Barresi Francesco Musumeci Maria Magdalena Mocanu Daniele F. Condorelli Ioan Ursu Rosaria Grasso Marisa Gulino Alexandru Garaiman Nicolò Musso Giuseppe A. Pablo Cirrone Giacomo Cuttone Detailed Analysis of Apoptosis and Delayed Luminescence of Human Leukemia Jurkat T Cells after Proton Irradiation and Treatments with Oxidant Agents and Flavonoids Oxidative Medicine and Cellular Longevity |
author_facet |
Irina Baran Constanta Ganea Simona Privitera Agata Scordino Vincenza Barresi Francesco Musumeci Maria Magdalena Mocanu Daniele F. Condorelli Ioan Ursu Rosaria Grasso Marisa Gulino Alexandru Garaiman Nicolò Musso Giuseppe A. Pablo Cirrone Giacomo Cuttone |
author_sort |
Irina Baran |
title |
Detailed Analysis of Apoptosis and Delayed Luminescence of Human Leukemia Jurkat T Cells after Proton Irradiation and Treatments with Oxidant Agents and Flavonoids |
title_short |
Detailed Analysis of Apoptosis and Delayed Luminescence of Human Leukemia Jurkat T Cells after Proton Irradiation and Treatments with Oxidant Agents and Flavonoids |
title_full |
Detailed Analysis of Apoptosis and Delayed Luminescence of Human Leukemia Jurkat T Cells after Proton Irradiation and Treatments with Oxidant Agents and Flavonoids |
title_fullStr |
Detailed Analysis of Apoptosis and Delayed Luminescence of Human Leukemia Jurkat T Cells after Proton Irradiation and Treatments with Oxidant Agents and Flavonoids |
title_full_unstemmed |
Detailed Analysis of Apoptosis and Delayed Luminescence of Human Leukemia Jurkat T Cells after Proton Irradiation and Treatments with Oxidant Agents and Flavonoids |
title_sort |
detailed analysis of apoptosis and delayed luminescence of human leukemia jurkat t cells after proton irradiation and treatments with oxidant agents and flavonoids |
publisher |
Hindawi Limited |
series |
Oxidative Medicine and Cellular Longevity |
issn |
1942-0900 1942-0994 |
publishDate |
2012-01-01 |
description |
Following previous work, we investigated in more detail the relationship between apoptosis and delayed luminescence (DL) in human leukemia Jurkat T cells under a wide variety of treatments. We used menadione and hydrogen peroxide to induce oxidative stress and two flavonoids, quercetin, and epigallocatechin gallate, applied alone or in combination with menadione or H2O2. 62 MeV proton beams were used to irradiate cells under a uniform dose of 2 or 10 Gy, respectively. We assessed apoptosis, cell cycle distributions, and DL. Menadione, H2O2 and quercetin were potent inducers of apoptosis and DL inhibitors. Quercetin decreased clonogenic survival and the NAD(P)H level in a dose-dependent manner. Proton irradiation with 2 Gy but not 10 Gy increased the apoptotic rate. However, both doses induced a substantial G2/M arrest. Quercetin reduced apoptosis and prolonged the G2/M arrest induced by radiation. DL spectroscopy indicated that proton irradiation disrupted the electron flow within Complex I of the mitochondrial respiratory chain, thus explaining the massive necrosis induced by 10 Gy of protons and also suggested an equivalent action of menadione and quercetin at the level of the Fe/S center N2, which may be mediated by their binding to a common site within Complex I, probably the rotenone-binding site. |
url |
http://dx.doi.org/10.1155/2012/498914 |
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