Enhanced Axial Resolution of Wide-Field Two-Photon Excitation Microscopy by Line Scanning Using a Digital Micromirror Device
Temporal focusing multiphoton microscopy is a technique for performing highly parallelized multiphoton microscopy while still maintaining depth discrimination. While the conventional wide-field configuration for temporal focusing suffers from sub-optimal axial resolution, line scanning temporal focu...
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doaj-2a4b734ec159461499bd3fca91fb42712020-11-24T23:49:56ZengMDPI AGMicromachines2072-666X2017-03-01838510.3390/mi8030085mi8030085Enhanced Axial Resolution of Wide-Field Two-Photon Excitation Microscopy by Line Scanning Using a Digital Micromirror DeviceJong Kang Park0Christopher J. Rowlands1Peter T. C. So2Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USADepartment of Chemical Engineering and Biotechnology, Cambridge University, Cambridge CB2 1TN, UKDepartment of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USATemporal focusing multiphoton microscopy is a technique for performing highly parallelized multiphoton microscopy while still maintaining depth discrimination. While the conventional wide-field configuration for temporal focusing suffers from sub-optimal axial resolution, line scanning temporal focusing, implemented here using a digital micromirror device (DMD), can provide substantial improvement. The DMD-based line scanning temporal focusing technique dynamically trades off the degree of parallelization, and hence imaging speed, for axial resolution, allowing performance parameters to be adapted to the experimental requirements. We demonstrate this new instrument in calibration specimens and in biological specimens, including a mouse kidney slice.http://www.mdpi.com/2072-666X/8/3/85multiphoton microscopytemporal focusingdigital micromirror device |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jong Kang Park Christopher J. Rowlands Peter T. C. So |
spellingShingle |
Jong Kang Park Christopher J. Rowlands Peter T. C. So Enhanced Axial Resolution of Wide-Field Two-Photon Excitation Microscopy by Line Scanning Using a Digital Micromirror Device Micromachines multiphoton microscopy temporal focusing digital micromirror device |
author_facet |
Jong Kang Park Christopher J. Rowlands Peter T. C. So |
author_sort |
Jong Kang Park |
title |
Enhanced Axial Resolution of Wide-Field Two-Photon Excitation Microscopy by Line Scanning Using a Digital Micromirror Device |
title_short |
Enhanced Axial Resolution of Wide-Field Two-Photon Excitation Microscopy by Line Scanning Using a Digital Micromirror Device |
title_full |
Enhanced Axial Resolution of Wide-Field Two-Photon Excitation Microscopy by Line Scanning Using a Digital Micromirror Device |
title_fullStr |
Enhanced Axial Resolution of Wide-Field Two-Photon Excitation Microscopy by Line Scanning Using a Digital Micromirror Device |
title_full_unstemmed |
Enhanced Axial Resolution of Wide-Field Two-Photon Excitation Microscopy by Line Scanning Using a Digital Micromirror Device |
title_sort |
enhanced axial resolution of wide-field two-photon excitation microscopy by line scanning using a digital micromirror device |
publisher |
MDPI AG |
series |
Micromachines |
issn |
2072-666X |
publishDate |
2017-03-01 |
description |
Temporal focusing multiphoton microscopy is a technique for performing highly parallelized multiphoton microscopy while still maintaining depth discrimination. While the conventional wide-field configuration for temporal focusing suffers from sub-optimal axial resolution, line scanning temporal focusing, implemented here using a digital micromirror device (DMD), can provide substantial improvement. The DMD-based line scanning temporal focusing technique dynamically trades off the degree of parallelization, and hence imaging speed, for axial resolution, allowing performance parameters to be adapted to the experimental requirements. We demonstrate this new instrument in calibration specimens and in biological specimens, including a mouse kidney slice. |
topic |
multiphoton microscopy temporal focusing digital micromirror device |
url |
http://www.mdpi.com/2072-666X/8/3/85 |
work_keys_str_mv |
AT jongkangpark enhancedaxialresolutionofwidefieldtwophotonexcitationmicroscopybylinescanningusingadigitalmicromirrordevice AT christopherjrowlands enhancedaxialresolutionofwidefieldtwophotonexcitationmicroscopybylinescanningusingadigitalmicromirrordevice AT petertcso enhancedaxialresolutionofwidefieldtwophotonexcitationmicroscopybylinescanningusingadigitalmicromirrordevice |
_version_ |
1725480778490445824 |