Study on the sensitivity of primary glioma cell to chemotherapeutic agents in vitro
Objective To study the sensitivity of primary human glioma cell to chemotherapeutic agents in vitro in order to provide an experimental basis for clinical chemotherapy of glioma. Methods The glioma cells derived from 46 cases of fresh brain glioma specimens were cultured in vitro. The sensitivity of...
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Tianjin Huanhu Hospital
2010-10-01
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doaj-2a54d175e2a641e4a07b0f23abacb3112020-11-24T21:27:40ZengTianjin Huanhu HospitalChinese Journal of Contemporary Neurology and Neurosurgery1672-67312010-10-01105540543505Study on the sensitivity of primary glioma cell to chemotherapeutic agents in vitroNing WANGWei WANGRi⁃xiao YANGMao⁃de WANGTuo WANGWei CHENQi LIObjective To study the sensitivity of primary human glioma cell to chemotherapeutic agents in vitro in order to provide an experimental basis for clinical chemotherapy of glioma. Methods The glioma cells derived from 46 cases of fresh brain glioma specimens were cultured in vitro. The sensitivity of primary cultured brain glioma cell to 4 chemotherapeutic agents which were teniposide (VM26), nimustine (ACNU), methotrexate (MTX) and temozolomide (TMZ) was determined by flow cytometry assay. Results The sensitivity sequence in 46 cases of 4 chemotherapeutic agents from high to low was VM26 (39.13%), TMZ (34.78%), ACNU (26.09%) and MTX (6.52%), there was no significant difference in the chemosensitivity in the groups of VM26, ACNU and TMZ. The chemosensitivity in MTX group was significant lower than the other groups ( χ2 = 13.883, 11.210, 6.452; P < 0.05, for all). There was no significant influence of patients' sex and age on chemosensitivity (P > 0.05, for all). Except TMZ group, the other chemotherapeutic agents showed no significant correlation of chemosensitivity with the pathological grade of the tumor. Conclusion Glioma sensitivity to chemotherapeutic drugs in vitro is different. VM26 anti⁃glioma effect is stronger than the other 3 drugs; TMZ shows a better effect in high grade gliomas. Flow cytometry assay is a rapid drug sensitivity detecting method for screening chemotherapy drugs. DOI:10.3969/j.issn.1672-6731.2010.05.008http://www.cjcnn.org/index.php/cjcnn/article/view/506GliomaAntineoplastic combined chemotherapy protocolsDrug screening assays, antitumorFlow cytometry |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ning WANG Wei WANG Ri⁃xiao YANG Mao⁃de WANG Tuo WANG Wei CHEN Qi LI |
spellingShingle |
Ning WANG Wei WANG Ri⁃xiao YANG Mao⁃de WANG Tuo WANG Wei CHEN Qi LI Study on the sensitivity of primary glioma cell to chemotherapeutic agents in vitro Chinese Journal of Contemporary Neurology and Neurosurgery Glioma Antineoplastic combined chemotherapy protocols Drug screening assays, antitumor Flow cytometry |
author_facet |
Ning WANG Wei WANG Ri⁃xiao YANG Mao⁃de WANG Tuo WANG Wei CHEN Qi LI |
author_sort |
Ning WANG |
title |
Study on the sensitivity of primary glioma cell to chemotherapeutic agents in vitro |
title_short |
Study on the sensitivity of primary glioma cell to chemotherapeutic agents in vitro |
title_full |
Study on the sensitivity of primary glioma cell to chemotherapeutic agents in vitro |
title_fullStr |
Study on the sensitivity of primary glioma cell to chemotherapeutic agents in vitro |
title_full_unstemmed |
Study on the sensitivity of primary glioma cell to chemotherapeutic agents in vitro |
title_sort |
study on the sensitivity of primary glioma cell to chemotherapeutic agents in vitro |
publisher |
Tianjin Huanhu Hospital |
series |
Chinese Journal of Contemporary Neurology and Neurosurgery |
issn |
1672-6731 |
publishDate |
2010-10-01 |
description |
Objective To study the sensitivity of primary human glioma cell to chemotherapeutic agents in vitro in order to provide an experimental basis for clinical chemotherapy of glioma. Methods The glioma cells derived from 46 cases of fresh brain glioma specimens were cultured in vitro. The sensitivity of primary cultured brain glioma cell to 4 chemotherapeutic agents which were teniposide (VM26), nimustine (ACNU), methotrexate (MTX) and temozolomide (TMZ) was determined by flow cytometry assay. Results The sensitivity sequence in 46 cases of 4 chemotherapeutic agents from high to low was VM26 (39.13%), TMZ (34.78%), ACNU (26.09%) and MTX (6.52%), there was no significant difference in the chemosensitivity in the groups of VM26, ACNU and TMZ. The chemosensitivity in MTX group was significant lower than the other groups ( χ2 = 13.883, 11.210, 6.452; P < 0.05, for all). There was no significant influence of patients' sex and age on chemosensitivity (P > 0.05, for all). Except TMZ group, the other chemotherapeutic agents showed no significant correlation of chemosensitivity with the pathological grade of the tumor. Conclusion Glioma sensitivity to chemotherapeutic drugs in vitro is different. VM26 anti⁃glioma effect is stronger than the other 3 drugs; TMZ shows a better effect in high grade gliomas. Flow cytometry assay is a rapid drug sensitivity detecting method for screening chemotherapy drugs.
DOI:10.3969/j.issn.1672-6731.2010.05.008 |
topic |
Glioma Antineoplastic combined chemotherapy protocols Drug screening assays, antitumor Flow cytometry |
url |
http://www.cjcnn.org/index.php/cjcnn/article/view/506 |
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