Marking <i>Drosophila suzukii</i> (Diptera: Drosophilidae) with Fluorescent Dusts
The marking of <i>Drosophila suzukii</i> can be an important instrument for studying the ecology and behaviour of this economically important fruit pest, aiding the development of new Integrated Pest Management (IPM) tools or strategies. There is, however, a need for a cost-effective met...
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doaj-2a5f844c075a47bf9dabaab6ea1e55092020-11-24T21:54:16ZengMDPI AGInsects2075-44502020-03-0111315210.3390/insects11030152insects11030152Marking <i>Drosophila suzukii</i> (Diptera: Drosophilidae) with Fluorescent DustsRik Clymans0Vincent Van Kerckvoorde1Tim Beliën2Dany Bylemans3Patrick De Clercq4Zoology Department, Research Centre for Fruit Cultivation (pcfruit npo), Fruittuinweg 1, B-3800 Sint-Truiden, BelgiumZoology Department, Research Centre for Fruit Cultivation (pcfruit npo), Fruittuinweg 1, B-3800 Sint-Truiden, BelgiumZoology Department, Research Centre for Fruit Cultivation (pcfruit npo), Fruittuinweg 1, B-3800 Sint-Truiden, BelgiumZoology Department, Research Centre for Fruit Cultivation (pcfruit npo), Fruittuinweg 1, B-3800 Sint-Truiden, BelgiumDepartment of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, B-9000 Ghent, BelgiumThe marking of <i>Drosophila suzukii</i> can be an important instrument for studying the ecology and behaviour of this economically important fruit pest, aiding the development of new Integrated Pest Management (IPM) tools or strategies. There is, however, a need for a cost-effective methodology that provides an easily detectable and stable mark. Whereas fluorescent pigment powders are often used in entomological research, the pigments (series, dyes), application techniques, or doses need to be evaluated for each studied species in terms of their efficacy and possible adverse effects on the performance of the insect. The effectiveness of different application techniques and dyes (RadGlo<sup>®</sup> TP-series) and their effect on the survival of adult <i>D. suzukii</i> were investigated in the laboratory. Furthermore, the influence of the marking on the behaviour of the flies was examined in laboratory trap assays (olfaction) and a field recapture study (general orientation). The persistence and detectability of the marks was evaluated both on living flies (for different application techniques) and dead flies under trapping/storage conditions. The use of fluorescent powders to mark <i>D. suzukii</i> flies yielded a clearly detectable and highly persistent mark, without any adverse effects on the survival and behaviour of the flies.https://www.mdpi.com/2075-4450/11/3/152<i>drosophila suzukii</i>insect markingmark-release-recapturemark-recapturedispersaltrappingradglo<sup>®</sup>dayglo<sup>®</sup>modellingbehaviour |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Rik Clymans Vincent Van Kerckvoorde Tim Beliën Dany Bylemans Patrick De Clercq |
spellingShingle |
Rik Clymans Vincent Van Kerckvoorde Tim Beliën Dany Bylemans Patrick De Clercq Marking <i>Drosophila suzukii</i> (Diptera: Drosophilidae) with Fluorescent Dusts Insects <i>drosophila suzukii</i> insect marking mark-release-recapture mark-recapture dispersal trapping radglo<sup>®</sup> dayglo<sup>®</sup> modelling behaviour |
author_facet |
Rik Clymans Vincent Van Kerckvoorde Tim Beliën Dany Bylemans Patrick De Clercq |
author_sort |
Rik Clymans |
title |
Marking <i>Drosophila suzukii</i> (Diptera: Drosophilidae) with Fluorescent Dusts |
title_short |
Marking <i>Drosophila suzukii</i> (Diptera: Drosophilidae) with Fluorescent Dusts |
title_full |
Marking <i>Drosophila suzukii</i> (Diptera: Drosophilidae) with Fluorescent Dusts |
title_fullStr |
Marking <i>Drosophila suzukii</i> (Diptera: Drosophilidae) with Fluorescent Dusts |
title_full_unstemmed |
Marking <i>Drosophila suzukii</i> (Diptera: Drosophilidae) with Fluorescent Dusts |
title_sort |
marking <i>drosophila suzukii</i> (diptera: drosophilidae) with fluorescent dusts |
publisher |
MDPI AG |
series |
Insects |
issn |
2075-4450 |
publishDate |
2020-03-01 |
description |
The marking of <i>Drosophila suzukii</i> can be an important instrument for studying the ecology and behaviour of this economically important fruit pest, aiding the development of new Integrated Pest Management (IPM) tools or strategies. There is, however, a need for a cost-effective methodology that provides an easily detectable and stable mark. Whereas fluorescent pigment powders are often used in entomological research, the pigments (series, dyes), application techniques, or doses need to be evaluated for each studied species in terms of their efficacy and possible adverse effects on the performance of the insect. The effectiveness of different application techniques and dyes (RadGlo<sup>®</sup> TP-series) and their effect on the survival of adult <i>D. suzukii</i> were investigated in the laboratory. Furthermore, the influence of the marking on the behaviour of the flies was examined in laboratory trap assays (olfaction) and a field recapture study (general orientation). The persistence and detectability of the marks was evaluated both on living flies (for different application techniques) and dead flies under trapping/storage conditions. The use of fluorescent powders to mark <i>D. suzukii</i> flies yielded a clearly detectable and highly persistent mark, without any adverse effects on the survival and behaviour of the flies. |
topic |
<i>drosophila suzukii</i> insect marking mark-release-recapture mark-recapture dispersal trapping radglo<sup>®</sup> dayglo<sup>®</sup> modelling behaviour |
url |
https://www.mdpi.com/2075-4450/11/3/152 |
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