Alkylation or Silylation for Analysis of Amino and Non-Amino Organic Acids by GC-MS?
Gas chromatography–mass spectrometry (GC-MS) is a widely used analytical technique in metabolomics. GC provides the highest resolution of any standard chromatographic separation method, and with modern instrumentation, retention times are very consistent between analyses. Electron impact ionization...
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doaj-2a68cce9d52f4006a7f4c5ab844bbc6d2020-11-24T21:14:49ZengMDPI AGMetabolites2218-19892011-01-011132010.3390/metabo1010003Alkylation or Silylation for Analysis of Amino and Non-Amino Organic Acids by GC-MS?Silas G. Villas-BôasKathleen F. SmartSubathira SivakumaranGeoffrey A. LaneGas chromatography–mass spectrometry (GC-MS) is a widely used analytical technique in metabolomics. GC provides the highest resolution of any standard chromatographic separation method, and with modern instrumentation, retention times are very consistent between analyses. Electron impact ionization and fragmentation is generally reproducible between instruments and extensive libraries of spectra are available that enhance the identification of analytes. The major limitation is the restriction to volatile analytes, and hence the requirement to convert many metabolites to volatile derivatives through chemical derivatization. Here we compared the analytical performance of two derivatization techniques, silylation (TMS) and alkylation (MCF), used for the analysis of amino and non-amino organic acids as well as nucleotides in microbial-derived samples. The widely used TMS derivatization method showed poorer reproducibility and instability during chromatographic runs while the MCF derivatives presented better analytical performance. Therefore, alkylation (MCF) derivatization seems to be preferable for the analysis of polyfunctional amines, nucleotides and organic acids in microbial metabolomics studies.http://www.mdpi.com/2218-1989/1/1/3Microbial metabolomicsmetabolite profilingmetabolomederivatizationgas chromatographymass spectrometrychroroformatesTMS |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Silas G. Villas-Bôas Kathleen F. Smart Subathira Sivakumaran Geoffrey A. Lane |
spellingShingle |
Silas G. Villas-Bôas Kathleen F. Smart Subathira Sivakumaran Geoffrey A. Lane Alkylation or Silylation for Analysis of Amino and Non-Amino Organic Acids by GC-MS? Metabolites Microbial metabolomics metabolite profiling metabolome derivatization gas chromatography mass spectrometry chroroformates TMS |
author_facet |
Silas G. Villas-Bôas Kathleen F. Smart Subathira Sivakumaran Geoffrey A. Lane |
author_sort |
Silas G. Villas-Bôas |
title |
Alkylation or Silylation for Analysis of Amino and Non-Amino Organic Acids by GC-MS? |
title_short |
Alkylation or Silylation for Analysis of Amino and Non-Amino Organic Acids by GC-MS? |
title_full |
Alkylation or Silylation for Analysis of Amino and Non-Amino Organic Acids by GC-MS? |
title_fullStr |
Alkylation or Silylation for Analysis of Amino and Non-Amino Organic Acids by GC-MS? |
title_full_unstemmed |
Alkylation or Silylation for Analysis of Amino and Non-Amino Organic Acids by GC-MS? |
title_sort |
alkylation or silylation for analysis of amino and non-amino organic acids by gc-ms? |
publisher |
MDPI AG |
series |
Metabolites |
issn |
2218-1989 |
publishDate |
2011-01-01 |
description |
Gas chromatography–mass spectrometry (GC-MS) is a widely used analytical technique in metabolomics. GC provides the highest resolution of any standard chromatographic separation method, and with modern instrumentation, retention times are very consistent between analyses. Electron impact ionization and fragmentation is generally reproducible between instruments and extensive libraries of spectra are available that enhance the identification of analytes. The major limitation is the restriction to volatile analytes, and hence the requirement to convert many metabolites to volatile derivatives through chemical derivatization. Here we compared the analytical performance of two derivatization techniques, silylation (TMS) and alkylation (MCF), used for the analysis of amino and non-amino organic acids as well as nucleotides in microbial-derived samples. The widely used TMS derivatization method showed poorer reproducibility and instability during chromatographic runs while the MCF derivatives presented better analytical performance. Therefore, alkylation (MCF) derivatization seems to be preferable for the analysis of polyfunctional amines, nucleotides and organic acids in microbial metabolomics studies. |
topic |
Microbial metabolomics metabolite profiling metabolome derivatization gas chromatography mass spectrometry chroroformates TMS |
url |
http://www.mdpi.com/2218-1989/1/1/3 |
work_keys_str_mv |
AT silasgvillasboas alkylationorsilylationforanalysisofaminoandnonaminoorganicacidsbygcms AT kathleenfsmart alkylationorsilylationforanalysisofaminoandnonaminoorganicacidsbygcms AT subathirasivakumaran alkylationorsilylationforanalysisofaminoandnonaminoorganicacidsbygcms AT geoffreyalane alkylationorsilylationforanalysisofaminoandnonaminoorganicacidsbygcms |
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