EBV-miR-BART7-3p Imposes Stemness in Nasopharyngeal Carcinoma Cells by Suppressing SMAD7
Cancer stem-like cells, possessing “stemness” properties, play crucial roles in progression, metastasis, and drug resistance in various cancers. Viral microRNAs (such as EBV-miR-BART7-3p), as exogenous regulators, have been discovered to regulate malignant progression of nasopharyngeal carcinoma (NP...
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Frontiers Media S.A.
2019-10-01
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Series: | Frontiers in Genetics |
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Online Access: | https://www.frontiersin.org/article/10.3389/fgene.2019.00939/full |
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doaj-2a777440771947bea41c5e66363da5c1 |
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record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Longmei Cai Longmei Cai Yufei Long Tuotuo Chong Wenzhi Cai Chi Man Tsang Xiaohan Zhou Yanling Lin Tengteng Ding Wenyan Zhou Hongli Zhao Yuxiang Chen Jianguo Wang Xiaoming Lyu William C. Cho Xin Li |
spellingShingle |
Longmei Cai Longmei Cai Yufei Long Tuotuo Chong Wenzhi Cai Chi Man Tsang Xiaohan Zhou Yanling Lin Tengteng Ding Wenyan Zhou Hongli Zhao Yuxiang Chen Jianguo Wang Xiaoming Lyu William C. Cho Xin Li EBV-miR-BART7-3p Imposes Stemness in Nasopharyngeal Carcinoma Cells by Suppressing SMAD7 Frontiers in Genetics EBV-miR-BART7-3p Epstein-Barr virus microRNA nasopharyngeal carcinoma SMAD7 stemness |
author_facet |
Longmei Cai Longmei Cai Yufei Long Tuotuo Chong Wenzhi Cai Chi Man Tsang Xiaohan Zhou Yanling Lin Tengteng Ding Wenyan Zhou Hongli Zhao Yuxiang Chen Jianguo Wang Xiaoming Lyu William C. Cho Xin Li |
author_sort |
Longmei Cai |
title |
EBV-miR-BART7-3p Imposes Stemness in Nasopharyngeal Carcinoma Cells by Suppressing SMAD7 |
title_short |
EBV-miR-BART7-3p Imposes Stemness in Nasopharyngeal Carcinoma Cells by Suppressing SMAD7 |
title_full |
EBV-miR-BART7-3p Imposes Stemness in Nasopharyngeal Carcinoma Cells by Suppressing SMAD7 |
title_fullStr |
EBV-miR-BART7-3p Imposes Stemness in Nasopharyngeal Carcinoma Cells by Suppressing SMAD7 |
title_full_unstemmed |
EBV-miR-BART7-3p Imposes Stemness in Nasopharyngeal Carcinoma Cells by Suppressing SMAD7 |
title_sort |
ebv-mir-bart7-3p imposes stemness in nasopharyngeal carcinoma cells by suppressing smad7 |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Genetics |
issn |
1664-8021 |
publishDate |
2019-10-01 |
description |
Cancer stem-like cells, possessing “stemness” properties, play crucial roles in progression, metastasis, and drug resistance in various cancers. Viral microRNAs (such as EBV-miR-BART7-3p), as exogenous regulators, have been discovered to regulate malignant progression of nasopharyngeal carcinoma (NPC), suggesting a possible role of viral microRNAs in imposing stemness. In this study, we found that EBV-miR-BART7-3p induce stemness of NPC cells. We firstly reported that EBV-miR-BART7-3p increased the percentage of side population cells, the development of tumor spheres, and the expression level of stemness markers in vitro. This viral microRNA also enhanced stem-like or cancer-initiating properties of NPC cells in vivo. Besides, we identified SMAD7 as a novel target gene of EBV-miR-BART7-3p in addition to PTEN gene we previously reported; this viral microRNA suppressed SMAD7, led to activation of TGF-β signaling, and eventually enhanced the stemness of NPC cells. Silencing of SMAD7 resembled the effects generated by EBV-miR-BART7-3p in NPC cells. After reconstitution of SMAD7, EBV-miR-BART7-3p-expressing cells underwent a phenotypic reversion. EBV-positive NPC cells were used to enable experimental validation. Finally, we further discovered that EBV-miR-BART7-3p increased chemo-resistance of NPC in vitro and in vivo, supporting that EBV-miR-BART7-3 resulted in increased stemness of NPC cells and lead to drug resistance and cancer recurrence. Overall, this study uncovered a novel mechanism underlying viral microRNA-associated stemness of NPC cells. This viral microRNA and its associated cellular genes may be potential therapeutic targets for restraining chemo-resistance and recurrence of NPC. |
topic |
EBV-miR-BART7-3p Epstein-Barr virus microRNA nasopharyngeal carcinoma SMAD7 stemness |
url |
https://www.frontiersin.org/article/10.3389/fgene.2019.00939/full |
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doaj-2a777440771947bea41c5e66363da5c12020-11-24T22:06:23ZengFrontiers Media S.A.Frontiers in Genetics1664-80212019-10-011010.3389/fgene.2019.00939463747EBV-miR-BART7-3p Imposes Stemness in Nasopharyngeal Carcinoma Cells by Suppressing SMAD7Longmei Cai0Longmei Cai1Yufei Long2Tuotuo Chong3Wenzhi Cai4Chi Man Tsang5Xiaohan Zhou6Yanling Lin7Tengteng Ding8Wenyan Zhou9Hongli Zhao10Yuxiang Chen11Jianguo Wang12Xiaoming Lyu13William C. Cho14Xin Li15Shenzhen Key Laboratory of Viral Oncology, The Clinical Innovation & Research Center (CIRC), Shenzhen Hospital, Southern Medical University, Shenzhen, ChinaDepartment of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaShenzhen Key Laboratory of Viral Oncology, The Clinical Innovation & Research Center (CIRC), Shenzhen Hospital, Southern Medical University, Shenzhen, ChinaShenzhen Key Laboratory of Viral Oncology, The Clinical Innovation & Research Center (CIRC), Shenzhen Hospital, Southern Medical University, Shenzhen, ChinaShenzhen Key Laboratory of Viral Oncology, The Clinical Innovation & Research Center (CIRC), Shenzhen Hospital, Southern Medical University, Shenzhen, ChinaDepartment of Anatomy and Center for Cancer Research, LKS Faculty of Medicine, The University of Hong Kong, Hong KongDepartment of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaShenzhen Key Laboratory of Viral Oncology, The Clinical Innovation & Research Center (CIRC), Shenzhen Hospital, Southern Medical University, Shenzhen, ChinaShenzhen Key Laboratory of Viral Oncology, The Clinical Innovation & Research Center (CIRC), Shenzhen Hospital, Southern Medical University, Shenzhen, ChinaShenzhen Key Laboratory of Viral Oncology, The Clinical Innovation & Research Center (CIRC), Shenzhen Hospital, Southern Medical University, Shenzhen, ChinaShenzhen Key Laboratory of Viral Oncology, The Clinical Innovation & Research Center (CIRC), Shenzhen Hospital, Southern Medical University, Shenzhen, ChinaShenzhen Key Laboratory of Viral Oncology, The Clinical Innovation & Research Center (CIRC), Shenzhen Hospital, Southern Medical University, Shenzhen, ChinaShenzhen Key Laboratory of Viral Oncology, The Clinical Innovation & Research Center (CIRC), Shenzhen Hospital, Southern Medical University, Shenzhen, ChinaDepartment of Clinical Oncology, Queen Elizabeth Hospital, Kowloon, Hong KongShenzhen Key Laboratory of Viral Oncology, The Clinical Innovation & Research Center (CIRC), Shenzhen Hospital, Southern Medical University, Shenzhen, ChinaCancer stem-like cells, possessing “stemness” properties, play crucial roles in progression, metastasis, and drug resistance in various cancers. Viral microRNAs (such as EBV-miR-BART7-3p), as exogenous regulators, have been discovered to regulate malignant progression of nasopharyngeal carcinoma (NPC), suggesting a possible role of viral microRNAs in imposing stemness. In this study, we found that EBV-miR-BART7-3p induce stemness of NPC cells. We firstly reported that EBV-miR-BART7-3p increased the percentage of side population cells, the development of tumor spheres, and the expression level of stemness markers in vitro. This viral microRNA also enhanced stem-like or cancer-initiating properties of NPC cells in vivo. Besides, we identified SMAD7 as a novel target gene of EBV-miR-BART7-3p in addition to PTEN gene we previously reported; this viral microRNA suppressed SMAD7, led to activation of TGF-β signaling, and eventually enhanced the stemness of NPC cells. Silencing of SMAD7 resembled the effects generated by EBV-miR-BART7-3p in NPC cells. After reconstitution of SMAD7, EBV-miR-BART7-3p-expressing cells underwent a phenotypic reversion. EBV-positive NPC cells were used to enable experimental validation. Finally, we further discovered that EBV-miR-BART7-3p increased chemo-resistance of NPC in vitro and in vivo, supporting that EBV-miR-BART7-3 resulted in increased stemness of NPC cells and lead to drug resistance and cancer recurrence. Overall, this study uncovered a novel mechanism underlying viral microRNA-associated stemness of NPC cells. This viral microRNA and its associated cellular genes may be potential therapeutic targets for restraining chemo-resistance and recurrence of NPC.https://www.frontiersin.org/article/10.3389/fgene.2019.00939/fullEBV-miR-BART7-3pEpstein-Barr virusmicroRNAnasopharyngeal carcinomaSMAD7stemness |