Bioassay-guided isolation of the antioxidant constituent from Cassia alata L. leaves
Using DPPH radical scavenging assay to investigate the antioxidant activity of crude methanol extracts from the leaves, flowers and pods of Cassia alata L. found that the leaf extract exhibited a stronger antioxidant activity than the extracts from the flowers and pods. On the basis of DPPH radical...
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Prince of Songkla University
2004-01-01
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Online Access: | http://www.sjst.psu.ac.th/journal/26-1.pdf/10Cassia%20alata%20L.%20leaves.pdf |
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doaj-2a81932e5e8a4f91bfd29e2ce3434cfb2020-11-24T22:41:52ZengPrince of Songkla UniversitySongklanakarin Journal of Science and Technology (SJST)0125-33952004-01-01261103107Bioassay-guided isolation of the antioxidant constituent from Cassia alata L. leavesPharkphoom PanichayupakaranantSongsri KaewsuwanUsing DPPH radical scavenging assay to investigate the antioxidant activity of crude methanol extracts from the leaves, flowers and pods of Cassia alata L. found that the leaf extract exhibited a stronger antioxidant activity than the extracts from the flowers and pods. On the basis of DPPH radical scavenging assay-guided isolation, the methanol extract of C. alata leaves was separated by silica gel vacuum chromatography and Sephadex LH-20 gel filtration chromatography afford a light yellowish powder (CA1), which was identified as kaempferol. This compound exhibited antioxidant activity (ED50 9.99 μM) that was six times stronger than that of BHT (ED50 57.41 μM) and fifty eight times stronger than that of emodin (ED50 578.87 μM).http://www.sjst.psu.ac.th/journal/26-1.pdf/10Cassia%20alata%20L.%20leaves.pdfCassia alataSenna alataantioxidantkaempferolemodin |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Pharkphoom Panichayupakaranant Songsri Kaewsuwan |
spellingShingle |
Pharkphoom Panichayupakaranant Songsri Kaewsuwan Bioassay-guided isolation of the antioxidant constituent from Cassia alata L. leaves Songklanakarin Journal of Science and Technology (SJST) Cassia alata Senna alata antioxidant kaempferol emodin |
author_facet |
Pharkphoom Panichayupakaranant Songsri Kaewsuwan |
author_sort |
Pharkphoom Panichayupakaranant |
title |
Bioassay-guided isolation of the antioxidant constituent from Cassia alata L. leaves |
title_short |
Bioassay-guided isolation of the antioxidant constituent from Cassia alata L. leaves |
title_full |
Bioassay-guided isolation of the antioxidant constituent from Cassia alata L. leaves |
title_fullStr |
Bioassay-guided isolation of the antioxidant constituent from Cassia alata L. leaves |
title_full_unstemmed |
Bioassay-guided isolation of the antioxidant constituent from Cassia alata L. leaves |
title_sort |
bioassay-guided isolation of the antioxidant constituent from cassia alata l. leaves |
publisher |
Prince of Songkla University |
series |
Songklanakarin Journal of Science and Technology (SJST) |
issn |
0125-3395 |
publishDate |
2004-01-01 |
description |
Using DPPH radical scavenging assay to investigate the antioxidant activity of crude methanol extracts from the leaves, flowers and pods of Cassia alata L. found that the leaf extract exhibited a stronger antioxidant activity than the extracts from the flowers and pods. On the basis of DPPH radical scavenging assay-guided isolation, the methanol extract of C. alata leaves was separated by silica gel vacuum chromatography and Sephadex LH-20 gel filtration chromatography afford a light yellowish powder (CA1), which was identified as kaempferol. This compound exhibited antioxidant activity (ED50 9.99 μM) that was six times stronger than that of BHT (ED50 57.41 μM) and fifty eight times stronger than that of emodin (ED50 578.87 μM). |
topic |
Cassia alata Senna alata antioxidant kaempferol emodin |
url |
http://www.sjst.psu.ac.th/journal/26-1.pdf/10Cassia%20alata%20L.%20leaves.pdf |
work_keys_str_mv |
AT pharkphoompanichayupakaranant bioassayguidedisolationoftheantioxidantconstituentfromcassiaalatalleaves AT songsrikaewsuwan bioassayguidedisolationoftheantioxidantconstituentfromcassiaalatalleaves |
_version_ |
1725700510550327296 |