Discovering aptamers by cell-SELEX against human soluble growth factors ectopically expressed on yeast cell surface.
SELEX, the process of selecting aptamers, is often hampered by the difficulty of preparing target molecules in their native forms and by a lack of a simple yet quantitative assay for monitoring enrichment and affinity of reactive aptamers. In this study, we sought to discover DNA aptamers against hu...
Main Authors: | Hsien-Wei Meng, John M Pagano, Brian S White, Yoshiko Toyoda, Irene M Min, Harold G Craighead, David Shalloway, John T Lis, Kai Xiao, Moonsoo M Jin |
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Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2014-01-01
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Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC3968096?pdf=render |
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