Aberrant Methylation of Aging-Related Genes in Asthma
Background: Asthma is a complex pulmonary inflammatory disease which is common among older adults. Aging-related alterations have also been found in structural cells and immune cells of asthma patients. Nonetheless, the underlying mechanism by which differenced aging-related gene contributes to asth...
Main Authors: | , , , , , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2021-05-01
|
Series: | Frontiers in Molecular Biosciences |
Subjects: | |
Online Access: | https://www.frontiersin.org/articles/10.3389/fmolb.2021.655285/full |
id |
doaj-2aa9fd3c67e04f7389430f2d6959ff98 |
---|---|
record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yu Yang Yu Yang Yu Yang Lin Yuan Ming Yang Xizi Du Ling Qin Ling Qin Leyuan Wang Kai Zhou Mengping Wu Ruoxi He Ruoxi He Juntao Feng Juntao Feng Yang Xiang Xiangping Qu Huijun Liu Xiaoqun Qin Chi Liu Chi Liu Chi Liu |
spellingShingle |
Yu Yang Yu Yang Yu Yang Lin Yuan Ming Yang Xizi Du Ling Qin Ling Qin Leyuan Wang Kai Zhou Mengping Wu Ruoxi He Ruoxi He Juntao Feng Juntao Feng Yang Xiang Xiangping Qu Huijun Liu Xiaoqun Qin Chi Liu Chi Liu Chi Liu Aberrant Methylation of Aging-Related Genes in Asthma Frontiers in Molecular Biosciences aging-related genes DNA methylation aging asthma allergy |
author_facet |
Yu Yang Yu Yang Yu Yang Lin Yuan Ming Yang Xizi Du Ling Qin Ling Qin Leyuan Wang Kai Zhou Mengping Wu Ruoxi He Ruoxi He Juntao Feng Juntao Feng Yang Xiang Xiangping Qu Huijun Liu Xiaoqun Qin Chi Liu Chi Liu Chi Liu |
author_sort |
Yu Yang |
title |
Aberrant Methylation of Aging-Related Genes in Asthma |
title_short |
Aberrant Methylation of Aging-Related Genes in Asthma |
title_full |
Aberrant Methylation of Aging-Related Genes in Asthma |
title_fullStr |
Aberrant Methylation of Aging-Related Genes in Asthma |
title_full_unstemmed |
Aberrant Methylation of Aging-Related Genes in Asthma |
title_sort |
aberrant methylation of aging-related genes in asthma |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Molecular Biosciences |
issn |
2296-889X |
publishDate |
2021-05-01 |
description |
Background: Asthma is a complex pulmonary inflammatory disease which is common among older adults. Aging-related alterations have also been found in structural cells and immune cells of asthma patients. Nonetheless, the underlying mechanism by which differenced aging-related gene contributes to asthma pathology remains unclear. Of note, DNA methylation (DNAm) has been proven to play a critical mechanism for age-related gene expression changes. However, the methylation changes of aging-related genes in asthma patients are still obscure.Methods: First, changes in DNAm and gene expression were detected with multiple targeted bisulfite enrichment sequencing (MethTarget) and qPCR in peripheral blood of 51 healthy controls (HCs) and 55 asthmatic patients. Second, the correlation between the DNAm levels of specific altered CpG sites and the pulmonary function indicators of asthma patients was evaluated. Last, the receiver operator characteristic (ROC) curve and principal component analysis (PCA) were used to identify the feasibility of the candidate CpG sites as biomarkers for asthma.Results: Compared with HCs, there was a differential mRNA expression for nine aging-related genes in peripheral blood of asthma patients. Besides, the methylation levels of the nine aging-related genes were also altered in asthma patients, and a total of 68 CpG sites were associated with the severity of asthma. Notably, 9 of the 68 CpG sites were significantly associated with pulmonary function parameters. Moreover, ROC curve and PCA analysis showed that the candidate differential methylation sites (DMSs) can be used as potential biomarkers for asthma.Conclusions: In summary, this study confirmed the differentially expressed mRNA and aberrant DNAm level of aging-related genes in asthma patients. DMSs are associated with the clinical evaluation indicators of asthma, which indicate the involvement of aging-related genes in the pathogenesis of asthma and provide some new possible biomarkers for asthma. |
topic |
aging-related genes DNA methylation aging asthma allergy |
url |
https://www.frontiersin.org/articles/10.3389/fmolb.2021.655285/full |
work_keys_str_mv |
AT yuyang aberrantmethylationofagingrelatedgenesinasthma AT yuyang aberrantmethylationofagingrelatedgenesinasthma AT yuyang aberrantmethylationofagingrelatedgenesinasthma AT linyuan aberrantmethylationofagingrelatedgenesinasthma AT mingyang aberrantmethylationofagingrelatedgenesinasthma AT xizidu aberrantmethylationofagingrelatedgenesinasthma AT lingqin aberrantmethylationofagingrelatedgenesinasthma AT lingqin aberrantmethylationofagingrelatedgenesinasthma AT leyuanwang aberrantmethylationofagingrelatedgenesinasthma AT kaizhou aberrantmethylationofagingrelatedgenesinasthma AT mengpingwu aberrantmethylationofagingrelatedgenesinasthma AT ruoxihe aberrantmethylationofagingrelatedgenesinasthma AT ruoxihe aberrantmethylationofagingrelatedgenesinasthma AT juntaofeng aberrantmethylationofagingrelatedgenesinasthma AT juntaofeng aberrantmethylationofagingrelatedgenesinasthma AT yangxiang aberrantmethylationofagingrelatedgenesinasthma AT xiangpingqu aberrantmethylationofagingrelatedgenesinasthma AT huijunliu aberrantmethylationofagingrelatedgenesinasthma AT xiaoqunqin aberrantmethylationofagingrelatedgenesinasthma AT chiliu aberrantmethylationofagingrelatedgenesinasthma AT chiliu aberrantmethylationofagingrelatedgenesinasthma AT chiliu aberrantmethylationofagingrelatedgenesinasthma |
_version_ |
1721427847105478656 |
spelling |
doaj-2aa9fd3c67e04f7389430f2d6959ff982021-05-25T06:35:08ZengFrontiers Media S.A.Frontiers in Molecular Biosciences2296-889X2021-05-01810.3389/fmolb.2021.655285655285Aberrant Methylation of Aging-Related Genes in AsthmaYu Yang0Yu Yang1Yu Yang2Lin Yuan3Ming Yang4Xizi Du5Ling Qin6Ling Qin7Leyuan Wang8Kai Zhou9Mengping Wu10Ruoxi He11Ruoxi He12Juntao Feng13Juntao Feng14Yang Xiang15Xiangping Qu16Huijun Liu17Xiaoqun Qin18Chi Liu19Chi Liu20Chi Liu21Department of Respiratory Medicine, National Clinical Research Center for Respiratory Diseases, Xiangya Hospital, Central South University, Changsha, ChinaDepartment of Physiology, School of Basic Medicine Science, Central South University, Changsha, ChinaBasic and Clinical Research Laboratory of Major Respiratory Diseases, Central South University, Changsha, ChinaDepartment of Physiology, School of Basic Medicine Science, Central South University, Changsha, ChinaCentre for Asthma and Respiratory Disease, School of Biomedical Sciences and Pharmacy, Faculty of Health and Medicine, University of Newcastle and Hunter Medical Research Institute, Callaghan, NSW, AustraliaDepartment of Physiology, School of Basic Medicine Science, Central South University, Changsha, ChinaDepartment of Respiratory Medicine, National Clinical Research Center for Respiratory Diseases, Xiangya Hospital, Central South University, Changsha, ChinaBasic and Clinical Research Laboratory of Major Respiratory Diseases, Central South University, Changsha, ChinaDepartment of Physiology, School of Basic Medicine Science, Central South University, Changsha, ChinaDepartment of Physiology, School of Basic Medicine Science, Central South University, Changsha, ChinaDepartment of Physiology, School of Basic Medicine Science, Central South University, Changsha, ChinaDepartment of Respiratory Medicine, National Clinical Research Center for Respiratory Diseases, Xiangya Hospital, Central South University, Changsha, ChinaBasic and Clinical Research Laboratory of Major Respiratory Diseases, Central South University, Changsha, ChinaDepartment of Respiratory Medicine, National Clinical Research Center for Respiratory Diseases, Xiangya Hospital, Central South University, Changsha, ChinaBasic and Clinical Research Laboratory of Major Respiratory Diseases, Central South University, Changsha, ChinaDepartment of Physiology, School of Basic Medicine Science, Central South University, Changsha, ChinaDepartment of Physiology, School of Basic Medicine Science, Central South University, Changsha, ChinaDepartment of Physiology, School of Basic Medicine Science, Central South University, Changsha, ChinaDepartment of Physiology, School of Basic Medicine Science, Central South University, Changsha, ChinaDepartment of Respiratory Medicine, National Clinical Research Center for Respiratory Diseases, Xiangya Hospital, Central South University, Changsha, ChinaDepartment of Physiology, School of Basic Medicine Science, Central South University, Changsha, ChinaResearch Center of China-Africa Infectious Diseases, Xiangya School of Medicine Central South University, Changsha, ChinaBackground: Asthma is a complex pulmonary inflammatory disease which is common among older adults. Aging-related alterations have also been found in structural cells and immune cells of asthma patients. Nonetheless, the underlying mechanism by which differenced aging-related gene contributes to asthma pathology remains unclear. Of note, DNA methylation (DNAm) has been proven to play a critical mechanism for age-related gene expression changes. However, the methylation changes of aging-related genes in asthma patients are still obscure.Methods: First, changes in DNAm and gene expression were detected with multiple targeted bisulfite enrichment sequencing (MethTarget) and qPCR in peripheral blood of 51 healthy controls (HCs) and 55 asthmatic patients. Second, the correlation between the DNAm levels of specific altered CpG sites and the pulmonary function indicators of asthma patients was evaluated. Last, the receiver operator characteristic (ROC) curve and principal component analysis (PCA) were used to identify the feasibility of the candidate CpG sites as biomarkers for asthma.Results: Compared with HCs, there was a differential mRNA expression for nine aging-related genes in peripheral blood of asthma patients. Besides, the methylation levels of the nine aging-related genes were also altered in asthma patients, and a total of 68 CpG sites were associated with the severity of asthma. Notably, 9 of the 68 CpG sites were significantly associated with pulmonary function parameters. Moreover, ROC curve and PCA analysis showed that the candidate differential methylation sites (DMSs) can be used as potential biomarkers for asthma.Conclusions: In summary, this study confirmed the differentially expressed mRNA and aberrant DNAm level of aging-related genes in asthma patients. DMSs are associated with the clinical evaluation indicators of asthma, which indicate the involvement of aging-related genes in the pathogenesis of asthma and provide some new possible biomarkers for asthma.https://www.frontiersin.org/articles/10.3389/fmolb.2021.655285/fullaging-related genesDNA methylationagingasthmaallergy |