In vivo oxalate degradation by liposome encapsulated oxalate oxidase in rat model of hyperoxaluria
Background & objectives: High level of urinary oxalate substantially increases the risk of hyperoxaluria, a significant risk factor for urolithiasis. The primary goal of this study was to reduce urinary oxalate excretion employing liposome encapsulated oxalate oxidase in animal model. Methods:...
Main Authors: | , |
---|---|
Format: | Article |
Language: | English |
Published: |
Wolters Kluwer Medknow Publications
2013-01-01
|
Series: | Indian Journal of Medical Research |
Subjects: | |
Online Access: | http://www.ijmr.org.in/article.asp?issn=0971-5916;year=2013;volume=137;issue=1;spage=136;epage=141;aulast=Dahiya |
id |
doaj-2ad4c02b9f724c0db7170dffc152db53 |
---|---|
record_format |
Article |
spelling |
doaj-2ad4c02b9f724c0db7170dffc152db532020-11-24T23:30:41ZengWolters Kluwer Medknow PublicationsIndian Journal of Medical Research0971-59162013-01-011371136141In vivo oxalate degradation by liposome encapsulated oxalate oxidase in rat model of hyperoxaluriaTulika DahiyaC s PundirBackground & objectives: High level of urinary oxalate substantially increases the risk of hyperoxaluria, a significant risk factor for urolithiasis. The primary goal of this study was to reduce urinary oxalate excretion employing liposome encapsulated oxalate oxidase in animal model. Methods: A membrane bound oxalate oxidase was purified from Bougainvillea leaves. The enzyme in its native form was less effective at the physiological pH of the recipient animal. To increase its functional viability, the enzyme was immobilized on to ethylene maleic anhydride (EMA). Rats were injected with liposome encapsulated EMA- oxalate oxidase and the effect was observed on degradation of oxalic acid. Results: The enzyme was purified to apparent homogeneity with 60-fold purification and 31 per cent yield. The optimum pH of EMA-derivative enzyme was 6.0 and it showed 70 per cent of its optimal activity at pH 7.0. The EMA-bound enzyme encapsulated into liposome showed greater oxalate degradation in 15 per cent casein vitamin B 6 deficient fed rats as compared with 30 per cent casein vitamin B 6 deficient fed rats and control rats. Interpretation & conclusions: EMA-oxalate oxidase encapsulated liposome caused oxalate degradation in experimental hyperoxaluria indicating that the enzyme could be used as a therapeutic agent in hyperoxaluria leading to urinary stones.http://www.ijmr.org.in/article.asp?issn=0971-5916;year=2013;volume=137;issue=1;spage=136;epage=141;aulast=DahiyaEnzyme therapy - hyperoxaluria - liposome - oxalate degradation - oxalate oxidase |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Tulika Dahiya C s Pundir |
spellingShingle |
Tulika Dahiya C s Pundir In vivo oxalate degradation by liposome encapsulated oxalate oxidase in rat model of hyperoxaluria Indian Journal of Medical Research Enzyme therapy - hyperoxaluria - liposome - oxalate degradation - oxalate oxidase |
author_facet |
Tulika Dahiya C s Pundir |
author_sort |
Tulika Dahiya |
title |
In vivo oxalate degradation by liposome encapsulated oxalate oxidase in rat model of hyperoxaluria |
title_short |
In vivo oxalate degradation by liposome encapsulated oxalate oxidase in rat model of hyperoxaluria |
title_full |
In vivo oxalate degradation by liposome encapsulated oxalate oxidase in rat model of hyperoxaluria |
title_fullStr |
In vivo oxalate degradation by liposome encapsulated oxalate oxidase in rat model of hyperoxaluria |
title_full_unstemmed |
In vivo oxalate degradation by liposome encapsulated oxalate oxidase in rat model of hyperoxaluria |
title_sort |
in vivo oxalate degradation by liposome encapsulated oxalate oxidase in rat model of hyperoxaluria |
publisher |
Wolters Kluwer Medknow Publications |
series |
Indian Journal of Medical Research |
issn |
0971-5916 |
publishDate |
2013-01-01 |
description |
Background & objectives: High level of urinary oxalate substantially increases the risk of hyperoxaluria, a significant risk factor for urolithiasis. The primary goal of this study was to reduce urinary oxalate excretion employing liposome encapsulated oxalate oxidase in animal model.
Methods: A membrane bound oxalate oxidase was purified from Bougainvillea leaves. The enzyme in its native form was less effective at the physiological pH of the recipient animal. To increase its functional viability, the enzyme was immobilized on to ethylene maleic anhydride (EMA). Rats were injected with liposome encapsulated EMA- oxalate oxidase and the effect was observed on degradation of oxalic acid.
Results: The enzyme was purified to apparent homogeneity with 60-fold purification and 31 per cent yield. The optimum pH of EMA-derivative enzyme was 6.0 and it showed 70 per cent of its optimal activity at pH 7.0. The EMA-bound enzyme encapsulated into liposome showed greater oxalate degradation in 15 per cent casein vitamin B 6 deficient fed rats as compared with 30 per cent casein vitamin B 6 deficient fed rats and control rats.
Interpretation & conclusions: EMA-oxalate oxidase encapsulated liposome caused oxalate degradation in experimental hyperoxaluria indicating that the enzyme could be used as a therapeutic agent in hyperoxaluria leading to urinary stones. |
topic |
Enzyme therapy - hyperoxaluria - liposome - oxalate degradation - oxalate oxidase |
url |
http://www.ijmr.org.in/article.asp?issn=0971-5916;year=2013;volume=137;issue=1;spage=136;epage=141;aulast=Dahiya |
work_keys_str_mv |
AT tulikadahiya invivooxalatedegradationbyliposomeencapsulatedoxalateoxidaseinratmodelofhyperoxaluria AT cspundir invivooxalatedegradationbyliposomeencapsulatedoxalateoxidaseinratmodelofhyperoxaluria |
_version_ |
1725540886443458560 |