Induced Pluripotent Stem Cell Models of Progranulin-Deficient Frontotemporal Dementia Uncover Specific Reversible Neuronal Defects
The pathogenic mechanisms of frontotemporal dementia (FTD) remain poorly understood. Here we generated multiple induced pluripotent stem cell lines from a control subject, a patient with sporadic FTD, and an FTD patient with a novel heterozygous GRN mutation (progranulin [PGRN] S116X). In neurons a...
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2012-10-01
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Series: | Cell Reports |
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doaj-2adf8552b17e40d2a9317f84afa9d11e2020-11-24T23:54:39ZengElsevierCell Reports2211-12472012-10-012478979810.1016/j.celrep.2012.09.007Induced Pluripotent Stem Cell Models of Progranulin-Deficient Frontotemporal Dementia Uncover Specific Reversible Neuronal DefectsSandra Almeida0Zhijun Zhang1Giovanni Coppola2Wenjie Mao3Kensuke Futai4Anna Karydas5Michael D. Geschwind6M. Carmela Tartaglia7Fuying Gao8Davide Gianni9Miguel Sena-Esteves10Daniel H. Geschwind11Bruce L. Miller12Robert V. Farese, Jr.13Fen-Biao Gao14Department of Neurology, University of Massachusetts Medical School, Worcester, MA 01605, USADepartment of Neurology, University of Massachusetts Medical School, Worcester, MA 01605, USADepartment of Neurology, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USABrudnick Neuropsychiatric Research Institute, Department of Psychiatry, University of Massachusetts Medical School, Worcester, MA 01605, USABrudnick Neuropsychiatric Research Institute, Department of Psychiatry, University of Massachusetts Medical School, Worcester, MA 01605, USAMemory and Aging Center, Department of Neurology, University of California, San Francisco, CA 94143, USAMemory and Aging Center, Department of Neurology, University of California, San Francisco, CA 94143, USAMemory and Aging Center, Department of Neurology, University of California, San Francisco, CA 94143, USADepartment of Neurology, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USADepartment of Neurology, University of Massachusetts Medical School, Worcester, MA 01605, USADepartment of Neurology, University of Massachusetts Medical School, Worcester, MA 01605, USADepartment of Neurology, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USABrudnick Neuropsychiatric Research Institute, Department of Psychiatry, University of Massachusetts Medical School, Worcester, MA 01605, USAGladstone Institute of Cardiovascular Disease, San Francisco, CA 94158, USADepartment of Neurology, University of Massachusetts Medical School, Worcester, MA 01605, USA The pathogenic mechanisms of frontotemporal dementia (FTD) remain poorly understood. Here we generated multiple induced pluripotent stem cell lines from a control subject, a patient with sporadic FTD, and an FTD patient with a novel heterozygous GRN mutation (progranulin [PGRN] S116X). In neurons and microglia differentiated from PGRN S116X induced pluripotent stem cells, the levels of intracellular and secreted PGRN were reduced, establishing patient-specific cellular models of PGRN haploinsufficiency. Through a systematic screen of inducers of cellular stress, we found that PGRN S116X neurons, but not sporadic FTD neurons, exhibited increased sensitivity to staurosporine and other kinase inhibitors. Moreover, the serine/threonine kinase S6K2, a component of the phosphatidylinositol 3-kinase and mitogen-activated protein kinase pathways, was specifically downregulated in PGRN S116X neurons. Both increased sensitivity to kinase inhibitors and reduced S6K2 were rescued by PGRN expression. Our findings identify cell-autonomous, reversible defects in patient neurons with PGRN deficiency, and provide a compelling model for studying PGRN-dependent pathogenic mechanisms and testing potential therapies. http://www.sciencedirect.com/science/article/pii/S2211124712002884 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sandra Almeida Zhijun Zhang Giovanni Coppola Wenjie Mao Kensuke Futai Anna Karydas Michael D. Geschwind M. Carmela Tartaglia Fuying Gao Davide Gianni Miguel Sena-Esteves Daniel H. Geschwind Bruce L. Miller Robert V. Farese, Jr. Fen-Biao Gao |
spellingShingle |
Sandra Almeida Zhijun Zhang Giovanni Coppola Wenjie Mao Kensuke Futai Anna Karydas Michael D. Geschwind M. Carmela Tartaglia Fuying Gao Davide Gianni Miguel Sena-Esteves Daniel H. Geschwind Bruce L. Miller Robert V. Farese, Jr. Fen-Biao Gao Induced Pluripotent Stem Cell Models of Progranulin-Deficient Frontotemporal Dementia Uncover Specific Reversible Neuronal Defects Cell Reports |
author_facet |
Sandra Almeida Zhijun Zhang Giovanni Coppola Wenjie Mao Kensuke Futai Anna Karydas Michael D. Geschwind M. Carmela Tartaglia Fuying Gao Davide Gianni Miguel Sena-Esteves Daniel H. Geschwind Bruce L. Miller Robert V. Farese, Jr. Fen-Biao Gao |
author_sort |
Sandra Almeida |
title |
Induced Pluripotent Stem Cell Models of Progranulin-Deficient Frontotemporal Dementia Uncover Specific Reversible Neuronal Defects |
title_short |
Induced Pluripotent Stem Cell Models of Progranulin-Deficient Frontotemporal Dementia Uncover Specific Reversible Neuronal Defects |
title_full |
Induced Pluripotent Stem Cell Models of Progranulin-Deficient Frontotemporal Dementia Uncover Specific Reversible Neuronal Defects |
title_fullStr |
Induced Pluripotent Stem Cell Models of Progranulin-Deficient Frontotemporal Dementia Uncover Specific Reversible Neuronal Defects |
title_full_unstemmed |
Induced Pluripotent Stem Cell Models of Progranulin-Deficient Frontotemporal Dementia Uncover Specific Reversible Neuronal Defects |
title_sort |
induced pluripotent stem cell models of progranulin-deficient frontotemporal dementia uncover specific reversible neuronal defects |
publisher |
Elsevier |
series |
Cell Reports |
issn |
2211-1247 |
publishDate |
2012-10-01 |
description |
The pathogenic mechanisms of frontotemporal dementia (FTD) remain poorly understood. Here we generated multiple induced pluripotent stem cell lines from a control subject, a patient with sporadic FTD, and an FTD patient with a novel heterozygous GRN mutation (progranulin [PGRN] S116X). In neurons and microglia differentiated from PGRN S116X induced pluripotent stem cells, the levels of intracellular and secreted PGRN were reduced, establishing patient-specific cellular models of PGRN haploinsufficiency. Through a systematic screen of inducers of cellular stress, we found that PGRN S116X neurons, but not sporadic FTD neurons, exhibited increased sensitivity to staurosporine and other kinase inhibitors. Moreover, the serine/threonine kinase S6K2, a component of the phosphatidylinositol 3-kinase and mitogen-activated protein kinase pathways, was specifically downregulated in PGRN S116X neurons. Both increased sensitivity to kinase inhibitors and reduced S6K2 were rescued by PGRN expression. Our findings identify cell-autonomous, reversible defects in patient neurons with PGRN deficiency, and provide a compelling model for studying PGRN-dependent pathogenic mechanisms and testing potential therapies.
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url |
http://www.sciencedirect.com/science/article/pii/S2211124712002884 |
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