The accuracy of histopathologic examination compared with polymerase chain reaction to diagnose human papillomavirus infection in the uterine cervix

• Main Author: I G.A.S. Mahendra Dewi
• Format: Article
• Language: English
• Published: DiscoverSys 2016-06-01
• Series: Bali Medical Journal
• Subjects: accuracy, histopathology, pcr, hpv, uterine cervix
• Online Access: https://balimedicaljournal.org/index.php/bmj/article/view/221

<p><strong>Introduction: </strong>In Indonesia and other developing countries cancer is still a health problem besides infectious disease. Cervical cancer is the second most common after breast cancer. Early detection of high risk Human Papillomavirus (HPV) infection as the causative agent is very important, so that cervical lesions do not develop into precancerous lesions and cancer. Polymerase Chain Reaction (PCR) at this time is still not yet possible be used as a screening program in the wider community because the cost is quite expensive. An easier, cheaper and simpler method of examination is needed to detect earlier infection, but still has accuracy close to gold standard. This study aims to determine the accuracy of histopathologic examination compared with PCR to diagnose HPV infection in the uterine cervix.</p><p><strong>Methods:</strong> This is a cross-sectional diagnostic test, with predictor variable is conventional histopathology examination and outcome variable is PCR examination. The samples in this study were tissue biopsy or surgery of patients with clinically diagnosed have cervical lesion. A 2x2 table was created to calculate the sensitivity (Se), specificity (Sp), positive predictive value (PPV) and negative predictive value (NPV).</p><p><strong>Result: </strong>A total of 39 samples were observed in this study. The sensitivity (Se), specificity (Sp), positive predictive value (PPV) and negative predictive value (NPV) were 72.73%, 58.82%, 69.57% and 62.50%, respectively.</p><p><strong>Conclusion:</strong> This study found the accuracy of histopathologic examination compared with PCR to diagnose HPV infection in the uterine cervix is more than 50%.</p>