Role of Mitochondrial Glycerol-3-Phosphate Dehydrogenase in Metabolic Adaptations of Prostate Cancer
Prostate cancer is one of the most prominent cancers diagnosed in males. Contrasting with other cancer types, glucose utilization is not increased in prostate carcinoma cells as they employ different metabolic adaptations involving mitochondria as a source of energy and intermediates required for ra...
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doaj-2b1e842ab00b46398a0f710c72fa72122020-11-25T03:14:46ZengMDPI AGCells2073-44092020-07-0191764176410.3390/cells9081764Role of Mitochondrial Glycerol-3-Phosphate Dehydrogenase in Metabolic Adaptations of Prostate CancerAlena Pecinová0Lukáš Alán1Andrea Brázdová2Marek Vrbacký3Petr Pecina4Zdeněk Drahota5Josef Houštěk6Tomáš Mráček7Department of Bioenergetics, Institute of Physiology, Czech Academy of Sciences, Videnska 1083, 142 20 Prague, Czech RepublicDepartment of Bioenergetics, Institute of Physiology, Czech Academy of Sciences, Videnska 1083, 142 20 Prague, Czech RepublicDepartment of Bioenergetics, Institute of Physiology, Czech Academy of Sciences, Videnska 1083, 142 20 Prague, Czech RepublicDepartment of Bioenergetics, Institute of Physiology, Czech Academy of Sciences, Videnska 1083, 142 20 Prague, Czech RepublicDepartment of Bioenergetics, Institute of Physiology, Czech Academy of Sciences, Videnska 1083, 142 20 Prague, Czech RepublicDepartment of Bioenergetics, Institute of Physiology, Czech Academy of Sciences, Videnska 1083, 142 20 Prague, Czech RepublicDepartment of Bioenergetics, Institute of Physiology, Czech Academy of Sciences, Videnska 1083, 142 20 Prague, Czech RepublicDepartment of Bioenergetics, Institute of Physiology, Czech Academy of Sciences, Videnska 1083, 142 20 Prague, Czech RepublicProstate cancer is one of the most prominent cancers diagnosed in males. Contrasting with other cancer types, glucose utilization is not increased in prostate carcinoma cells as they employ different metabolic adaptations involving mitochondria as a source of energy and intermediates required for rapid cell growth. In this regard, prostate cancer cells were associated with higher activity of mitochondrial glycerol-3-phosphate dehydrogenase (mGPDH), the key rate limiting component of the glycerophosphate shuttle, which connects mitochondrial and cytosolic processes and plays significant role in cellular bioenergetics. Our research focused on the role of mGPDH biogenesis and regulation in prostate cancer compared to healthy cells. We show that the 42 amino acid presequence is cleaved from N-terminus during mGPDH biogenesis. Only the processed form is part of the mGPDH dimer that is the prominent functional enzyme entity. We demonstrate that mGPDH overexpression enhances the wound healing ability in prostate cancer cells. As mGPDH is at the crossroad of glycolysis, lipogenesis and oxidative metabolism, regulation of its activity by intramitochondrial processing might represent rapid means of cellular metabolic adaptations.https://www.mdpi.com/2073-4409/9/8/1764GPD2 genemitochondrial glycerol-3-phosphate dehydrogenase (EC:1.1.5.3)prostate cancermetabolic adaptation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Alena Pecinová Lukáš Alán Andrea Brázdová Marek Vrbacký Petr Pecina Zdeněk Drahota Josef Houštěk Tomáš Mráček |
spellingShingle |
Alena Pecinová Lukáš Alán Andrea Brázdová Marek Vrbacký Petr Pecina Zdeněk Drahota Josef Houštěk Tomáš Mráček Role of Mitochondrial Glycerol-3-Phosphate Dehydrogenase in Metabolic Adaptations of Prostate Cancer Cells GPD2 gene mitochondrial glycerol-3-phosphate dehydrogenase (EC:1.1.5.3) prostate cancer metabolic adaptation |
author_facet |
Alena Pecinová Lukáš Alán Andrea Brázdová Marek Vrbacký Petr Pecina Zdeněk Drahota Josef Houštěk Tomáš Mráček |
author_sort |
Alena Pecinová |
title |
Role of Mitochondrial Glycerol-3-Phosphate Dehydrogenase in Metabolic Adaptations of Prostate Cancer |
title_short |
Role of Mitochondrial Glycerol-3-Phosphate Dehydrogenase in Metabolic Adaptations of Prostate Cancer |
title_full |
Role of Mitochondrial Glycerol-3-Phosphate Dehydrogenase in Metabolic Adaptations of Prostate Cancer |
title_fullStr |
Role of Mitochondrial Glycerol-3-Phosphate Dehydrogenase in Metabolic Adaptations of Prostate Cancer |
title_full_unstemmed |
Role of Mitochondrial Glycerol-3-Phosphate Dehydrogenase in Metabolic Adaptations of Prostate Cancer |
title_sort |
role of mitochondrial glycerol-3-phosphate dehydrogenase in metabolic adaptations of prostate cancer |
publisher |
MDPI AG |
series |
Cells |
issn |
2073-4409 |
publishDate |
2020-07-01 |
description |
Prostate cancer is one of the most prominent cancers diagnosed in males. Contrasting with other cancer types, glucose utilization is not increased in prostate carcinoma cells as they employ different metabolic adaptations involving mitochondria as a source of energy and intermediates required for rapid cell growth. In this regard, prostate cancer cells were associated with higher activity of mitochondrial glycerol-3-phosphate dehydrogenase (mGPDH), the key rate limiting component of the glycerophosphate shuttle, which connects mitochondrial and cytosolic processes and plays significant role in cellular bioenergetics. Our research focused on the role of mGPDH biogenesis and regulation in prostate cancer compared to healthy cells. We show that the 42 amino acid presequence is cleaved from N-terminus during mGPDH biogenesis. Only the processed form is part of the mGPDH dimer that is the prominent functional enzyme entity. We demonstrate that mGPDH overexpression enhances the wound healing ability in prostate cancer cells. As mGPDH is at the crossroad of glycolysis, lipogenesis and oxidative metabolism, regulation of its activity by intramitochondrial processing might represent rapid means of cellular metabolic adaptations. |
topic |
GPD2 gene mitochondrial glycerol-3-phosphate dehydrogenase (EC:1.1.5.3) prostate cancer metabolic adaptation |
url |
https://www.mdpi.com/2073-4409/9/8/1764 |
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