HnRNPA1 Specifically Recognizes the Base of Nucleotide at the Loop of RNA G-Quadruplex
Human telomere RNA performs various cellular functions, such as telomere length regulation, heterochromatin formation, and end protection. We recently demonstrated that the loops in the RNA G-quadruplex are important in the interaction of telomere RNA with heterogeneous nuclear ribonucleoprotein A1...
Main Authors: | , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2018-01-01
|
Series: | Molecules |
Subjects: | |
Online Access: | http://www.mdpi.com/1420-3049/23/1/237 |
id |
doaj-2b4ad4ff72924418888df3e8a49e8761 |
---|---|
record_format |
Article |
spelling |
doaj-2b4ad4ff72924418888df3e8a49e87612020-11-25T00:25:45ZengMDPI AGMolecules1420-30492018-01-0123123710.3390/molecules23010237molecules23010237HnRNPA1 Specifically Recognizes the Base of Nucleotide at the Loop of RNA G-QuadruplexXiao Liu0Yan Xu1Division of Chemistry, Department of Medical Sciences, Faculty of Medicine, University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki 889-1692, JapanDivision of Chemistry, Department of Medical Sciences, Faculty of Medicine, University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki 889-1692, JapanHuman telomere RNA performs various cellular functions, such as telomere length regulation, heterochromatin formation, and end protection. We recently demonstrated that the loops in the RNA G-quadruplex are important in the interaction of telomere RNA with heterogeneous nuclear ribonucleoprotein A1 (hnRNPA1). Here, we report on a detailed analysis of hnRNPA1 binding to telomere RNA G-quadruplexes with a group of loop variants using an electrophoretic mobility shift assay (EMSA) and circular dichroism (CD) spectroscopy. We found that the hnRNPA1 binds to RNA G-quadruplexes with the 2’-O-methyl and DNA loops, but fails to bind with the abasic RNA and DNA loops. These results suggested that hnRNPA1 binds to the loop of the RNA G-quadruplex by recognizing the base of the loop’s nucleotides. The observation provides the first evidence that the base of the loop’s nucleotides is a key factor for hnRNPA1 specifically recognizing the RNA G-quadruplex.http://www.mdpi.com/1420-3049/23/1/237hnRNPA1RNA G-quadruplextelomere RNAloop of RNA G-quadruplexbase |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xiao Liu Yan Xu |
spellingShingle |
Xiao Liu Yan Xu HnRNPA1 Specifically Recognizes the Base of Nucleotide at the Loop of RNA G-Quadruplex Molecules hnRNPA1 RNA G-quadruplex telomere RNA loop of RNA G-quadruplex base |
author_facet |
Xiao Liu Yan Xu |
author_sort |
Xiao Liu |
title |
HnRNPA1 Specifically Recognizes the Base of Nucleotide at the Loop of RNA G-Quadruplex |
title_short |
HnRNPA1 Specifically Recognizes the Base of Nucleotide at the Loop of RNA G-Quadruplex |
title_full |
HnRNPA1 Specifically Recognizes the Base of Nucleotide at the Loop of RNA G-Quadruplex |
title_fullStr |
HnRNPA1 Specifically Recognizes the Base of Nucleotide at the Loop of RNA G-Quadruplex |
title_full_unstemmed |
HnRNPA1 Specifically Recognizes the Base of Nucleotide at the Loop of RNA G-Quadruplex |
title_sort |
hnrnpa1 specifically recognizes the base of nucleotide at the loop of rna g-quadruplex |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2018-01-01 |
description |
Human telomere RNA performs various cellular functions, such as telomere length regulation, heterochromatin formation, and end protection. We recently demonstrated that the loops in the RNA G-quadruplex are important in the interaction of telomere RNA with heterogeneous nuclear ribonucleoprotein A1 (hnRNPA1). Here, we report on a detailed analysis of hnRNPA1 binding to telomere RNA G-quadruplexes with a group of loop variants using an electrophoretic mobility shift assay (EMSA) and circular dichroism (CD) spectroscopy. We found that the hnRNPA1 binds to RNA G-quadruplexes with the 2’-O-methyl and DNA loops, but fails to bind with the abasic RNA and DNA loops. These results suggested that hnRNPA1 binds to the loop of the RNA G-quadruplex by recognizing the base of the loop’s nucleotides. The observation provides the first evidence that the base of the loop’s nucleotides is a key factor for hnRNPA1 specifically recognizing the RNA G-quadruplex. |
topic |
hnRNPA1 RNA G-quadruplex telomere RNA loop of RNA G-quadruplex base |
url |
http://www.mdpi.com/1420-3049/23/1/237 |
work_keys_str_mv |
AT xiaoliu hnrnpa1specificallyrecognizesthebaseofnucleotideattheloopofrnagquadruplex AT yanxu hnrnpa1specificallyrecognizesthebaseofnucleotideattheloopofrnagquadruplex |
_version_ |
1725347109517918208 |