UBE2C regulates proliferation and invasion of neuroblastoma: An experimental study
Objective: To study the regulatory effect of ubiquitin-conjugating enzyme 2C (UBE2C) on the proliferation and invasion of neuroblastoma. Methods: SH-SY5Y neuroblastoma cell lines were cultured and randomly divided into UBE2C-siRNA group and NC-siRNA group that were transfected with UBE2C siRNA an...
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doaj-2b5fe9864214436ebcbb676c8c7e728d2020-11-25T00:43:17ZengEditorial Board of Journal of Hainan Medical UniversityJournal of Hainan Medical University1007-12371007-12372018-11-01241158UBE2C regulates proliferation and invasion of neuroblastoma: An experimental studyLi Feng0Department of Pediatric Surgery, the Central Hospital of Enshi Autonomous Prefecture Hubei Province, Enshi, Hubei Province, 445000Objective: To study the regulatory effect of ubiquitin-conjugating enzyme 2C (UBE2C) on the proliferation and invasion of neuroblastoma. Methods: SH-SY5Y neuroblastoma cell lines were cultured and randomly divided into UBE2C-siRNA group and NC-siRNA group that were transfected with UBE2C siRNA and NC siRNA respectively. 24 h after siRNA transfection, the RNA in the cells was extracted, and fluorescence quantitative PCR reaction was used to detect the mRNA expression of pro-proliferation genes YB-1, CyclinD1, CDK4, Aurora-A and Ki-67, anti-proliferation genes LC3, Beclin1, GRP78, IRE1α, PERK and ATF6 as well as invasion genes KLF4, RIPK3, HIF-1α, Integrin β1 and MMP9. Results: YB-1, CyclinD1, CDK4, Aurora-A, Ki-67, KLF4, RIPK3, HIF-1α, Integrin β1 and MMP9 mRNA expression in UBE2C-siRNA group of cells were significantly lower than those in NC-siRNA group whereas LC3, Beclin1, GRP78, IRE1α, PERK and ATF6 mRNA expression were significantly higher than those in NC-siRNA group. Conclusions: Inhibition of the UBE2C gene can regulate the expression of proliferation and invasion genes in neuroblastoma to hinder cell proliferation and invasion.http://www.hnykdxxb.com/PDF/201811/02.pdf NeuroblastomaUbiquitin-conjugating enzyme 2CProliferationInvasion |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Li Feng |
spellingShingle |
Li Feng UBE2C regulates proliferation and invasion of neuroblastoma: An experimental study Journal of Hainan Medical University Neuroblastoma Ubiquitin-conjugating enzyme 2C Proliferation Invasion |
author_facet |
Li Feng |
author_sort |
Li Feng |
title |
UBE2C regulates proliferation and invasion of neuroblastoma: An experimental study |
title_short |
UBE2C regulates proliferation and invasion of neuroblastoma: An experimental study |
title_full |
UBE2C regulates proliferation and invasion of neuroblastoma: An experimental study |
title_fullStr |
UBE2C regulates proliferation and invasion of neuroblastoma: An experimental study |
title_full_unstemmed |
UBE2C regulates proliferation and invasion of neuroblastoma: An experimental study |
title_sort |
ube2c regulates proliferation and invasion of neuroblastoma: an experimental study |
publisher |
Editorial Board of Journal of Hainan Medical University |
series |
Journal of Hainan Medical University |
issn |
1007-1237 1007-1237 |
publishDate |
2018-11-01 |
description |
Objective: To study the regulatory effect of ubiquitin-conjugating enzyme 2C (UBE2C) on
the proliferation and invasion of neuroblastoma. Methods: SH-SY5Y neuroblastoma cell
lines were cultured and randomly divided into UBE2C-siRNA group and NC-siRNA group
that were transfected with UBE2C siRNA and NC siRNA respectively. 24 h after siRNA
transfection, the RNA in the cells was extracted, and fluorescence quantitative PCR reaction
was used to detect the mRNA expression of pro-proliferation genes YB-1, CyclinD1, CDK4,
Aurora-A and Ki-67, anti-proliferation genes LC3, Beclin1, GRP78, IRE1α, PERK and ATF6
as well as invasion genes KLF4, RIPK3, HIF-1α, Integrin β1 and MMP9. Results: YB-1,
CyclinD1, CDK4, Aurora-A, Ki-67, KLF4, RIPK3, HIF-1α, Integrin β1 and MMP9 mRNA
expression in UBE2C-siRNA group of cells were significantly lower than those in NC-siRNA
group whereas LC3, Beclin1, GRP78, IRE1α, PERK and ATF6 mRNA expression were
significantly higher than those in NC-siRNA group. Conclusions: Inhibition of the UBE2C
gene can regulate the expression of proliferation and invasion genes in neuroblastoma to hinder
cell proliferation and invasion. |
topic |
Neuroblastoma Ubiquitin-conjugating enzyme 2C Proliferation Invasion |
url |
http://www.hnykdxxb.com/PDF/201811/02.pdf |
work_keys_str_mv |
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