UBE2C regulates proliferation and invasion of neuroblastoma: An experimental study

• Main Author: Li Feng
• Format: Article
• Language: English
• Published: Editorial Board of Journal of Hainan Medical University 2018-11-01
• Series: Journal of Hainan Medical University
• Subjects: Neuroblastoma; Ubiquitin-conjugating enzyme 2C; Proliferation; Invasion
• Online Access: http://www.hnykdxxb.com/PDF/201811/02.pdf

Objective: To study the regulatory effect of ubiquitin-conjugating enzyme 2C (UBE2C) on the proliferation and invasion of neuroblastoma. Methods: SH-SY5Y neuroblastoma cell lines were cultured and randomly divided into UBE2C-siRNA group and NC-siRNA group that were transfected with UBE2C siRNA and NC siRNA respectively. 24 h after siRNA transfection, the RNA in the cells was extracted, and fluorescence quantitative PCR reaction was used to detect the mRNA expression of pro-proliferation genes YB-1, CyclinD1, CDK4, Aurora-A and Ki-67, anti-proliferation genes LC3, Beclin1, GRP78, IRE1α, PERK and ATF6 as well as invasion genes KLF4, RIPK3, HIF-1α, Integrin β1 and MMP9. Results: YB-1, CyclinD1, CDK4, Aurora-A, Ki-67, KLF4, RIPK3, HIF-1α, Integrin β1 and MMP9 mRNA expression in UBE2C-siRNA group of cells were significantly lower than those in NC-siRNA group whereas LC3, Beclin1, GRP78, IRE1α, PERK and ATF6 mRNA expression were significantly higher than those in NC-siRNA group. Conclusions: Inhibition of the UBE2C gene can regulate the expression of proliferation and invasion genes in neuroblastoma to hinder cell proliferation and invasion.