Metabolic Profiling of Dendrobium officinale in Response to Precursors and Methyl Jasmonate
Alkaloids are the main active ingredients in the medicinal plant Dendrobium officinale. Based on the published genomic and transcriptomic data, a proposed terpenoid indole alkaloid (TIA) biosynthesis pathway may be present in D. officinale. In this study, protocorm-like bodies (PLBs) with a high-yie...
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doaj-2b8b270c75db4e6e84f886d55350d5202020-11-24T22:06:43ZengMDPI AGInternational Journal of Molecular Sciences1422-00672018-03-0119372810.3390/ijms19030728ijms19030728Metabolic Profiling of Dendrobium officinale in Response to Precursors and Methyl JasmonateChunyan Jiao0Cheng Song1Siyan Zheng2Yingpeng Zhu3Qing Jin4Yongping Cai5Yi Lin6School of Life Sciences, Anhui Agricultural University, Hefei 230036, ChinaSchool of Life Sciences, Anhui Agricultural University, Hefei 230036, ChinaSchool of Life Sciences, Anhui Agricultural University, Hefei 230036, ChinaSchool of Life Sciences, Anhui Agricultural University, Hefei 230036, ChinaSchool of Life Sciences, Anhui Agricultural University, Hefei 230036, ChinaSchool of Life Sciences, Anhui Agricultural University, Hefei 230036, ChinaSchool of Life Sciences, Anhui Agricultural University, Hefei 230036, ChinaAlkaloids are the main active ingredients in the medicinal plant Dendrobium officinale. Based on the published genomic and transcriptomic data, a proposed terpenoid indole alkaloid (TIA) biosynthesis pathway may be present in D. officinale. In this study, protocorm-like bodies (PLBs) with a high-yielding production of alkaloids were obtained by the optimization of tryptophan, secologanin and methyl jasmonate (MeJA) treatment. The results showed that the total alkaloid content was 2.05 times greater than that of the control group when the PLBs were fed with 9 µM tryptophan, 6 µM secologanin and 100 µM MeJA after 36 days. HPLC analysis showed that strictosidine synthase (STR) activity also increased in the treated plants. A total of 78 metabolites were identified using gas chromatography-mass spectrometry (GC-MS) in combination with liquid chromatography-mass spectrometry (LC-MS) methods; 29 differential metabolites were identified according to the multivariate statistical analysis. Among them, carapanaubine, a kind of TIA, exhibited dramatically increased levels. In addition, a possible underlying process of the metabolic flux from related metabolism to the TIA biosynthetic pathway was enhanced. These results provide a comprehensive view of the metabolic changes related to alkaloid biosynthesis, especially TIA biosynthesis, in response to tryptophan, secologanin and MeJA treatment.http://www.mdpi.com/1422-0067/19/3/728Dendrobium officinalemetabolomicsalkaloidstryptophansecologaninmethyl jasmonate |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Chunyan Jiao Cheng Song Siyan Zheng Yingpeng Zhu Qing Jin Yongping Cai Yi Lin |
spellingShingle |
Chunyan Jiao Cheng Song Siyan Zheng Yingpeng Zhu Qing Jin Yongping Cai Yi Lin Metabolic Profiling of Dendrobium officinale in Response to Precursors and Methyl Jasmonate International Journal of Molecular Sciences Dendrobium officinale metabolomics alkaloids tryptophan secologanin methyl jasmonate |
author_facet |
Chunyan Jiao Cheng Song Siyan Zheng Yingpeng Zhu Qing Jin Yongping Cai Yi Lin |
author_sort |
Chunyan Jiao |
title |
Metabolic Profiling of Dendrobium officinale in Response to Precursors and Methyl Jasmonate |
title_short |
Metabolic Profiling of Dendrobium officinale in Response to Precursors and Methyl Jasmonate |
title_full |
Metabolic Profiling of Dendrobium officinale in Response to Precursors and Methyl Jasmonate |
title_fullStr |
Metabolic Profiling of Dendrobium officinale in Response to Precursors and Methyl Jasmonate |
title_full_unstemmed |
Metabolic Profiling of Dendrobium officinale in Response to Precursors and Methyl Jasmonate |
title_sort |
metabolic profiling of dendrobium officinale in response to precursors and methyl jasmonate |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2018-03-01 |
description |
Alkaloids are the main active ingredients in the medicinal plant Dendrobium officinale. Based on the published genomic and transcriptomic data, a proposed terpenoid indole alkaloid (TIA) biosynthesis pathway may be present in D. officinale. In this study, protocorm-like bodies (PLBs) with a high-yielding production of alkaloids were obtained by the optimization of tryptophan, secologanin and methyl jasmonate (MeJA) treatment. The results showed that the total alkaloid content was 2.05 times greater than that of the control group when the PLBs were fed with 9 µM tryptophan, 6 µM secologanin and 100 µM MeJA after 36 days. HPLC analysis showed that strictosidine synthase (STR) activity also increased in the treated plants. A total of 78 metabolites were identified using gas chromatography-mass spectrometry (GC-MS) in combination with liquid chromatography-mass spectrometry (LC-MS) methods; 29 differential metabolites were identified according to the multivariate statistical analysis. Among them, carapanaubine, a kind of TIA, exhibited dramatically increased levels. In addition, a possible underlying process of the metabolic flux from related metabolism to the TIA biosynthetic pathway was enhanced. These results provide a comprehensive view of the metabolic changes related to alkaloid biosynthesis, especially TIA biosynthesis, in response to tryptophan, secologanin and MeJA treatment. |
topic |
Dendrobium officinale metabolomics alkaloids tryptophan secologanin methyl jasmonate |
url |
http://www.mdpi.com/1422-0067/19/3/728 |
work_keys_str_mv |
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1725822300002975744 |