Mummichog gill and operculum exhibit functionally consistent claudin-10 paralog profiles and Claudin-10c hypersaline response
Claudin (Cldn)-10 tight junction (TJ) proteins are hypothesized to form the paracellular Na+ secretion pathway of hyposmoregulating mummichog (Fundulus heteroclitus) branchial epithelia. Organ-specific expression profiles showed that only branchial organs [the gill and opercular epithelium (OE)] exh...
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The Company of Biologists
2021-07-01
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doaj-2b9e5cd5ff734565a376e38c44f24acb2021-09-19T12:57:44ZengThe Company of BiologistsBiology Open2046-63902021-07-0110710.1242/bio.058868058868Mummichog gill and operculum exhibit functionally consistent claudin-10 paralog profiles and Claudin-10c hypersaline responseChun Chih Chen0William S. Marshall1George N. Robertson2Regina R. F. Cozzi3Scott P. Kelly4 Department of Biology, York University, Toronto, ON M3J 1P3, Canada Department of Biology, St. Francis Xavier University, Antigonish, NS B2G 2W5, Canada Department of Biology, St. Francis Xavier University, Antigonish, NS B2G 2W5, Canada Department of Biology, St. Francis Xavier University, Antigonish, NS B2G 2W5, Canada Department of Biology, York University, Toronto, ON M3J 1P3, Canada Claudin (Cldn)-10 tight junction (TJ) proteins are hypothesized to form the paracellular Na+ secretion pathway of hyposmoregulating mummichog (Fundulus heteroclitus) branchial epithelia. Organ-specific expression profiles showed that only branchial organs [the gill and opercular epithelium (OE)] exhibited abundant cldn-10 paralog transcripts, which typically increased following seawater (SW) to hypersaline (2SW) challenge. Post-translational properties, protein abundance, and ionocyte localization of Cldn-10c, were then examined in gill and OE. Western blot analysis revealed two Cldn-10c immunoreactive bands in the mummichog gill and OE at ∼29 kDa and ∼40 kDa. The heavier protein could be eliminated by glycosidase treatment, demonstrating the novel presence of a glycosylated Cldn-10c. Protein abundance of Cldn-10c increased in gill and OE of 2SW-exposed fish. Cldn-10c localized to the sides of gill and OE ionocyte apical crypts and partially colocalized with cystic fibrosis transmembrane conductance regulator and F-actin, consistent with TJ complex localization. Cldn-10c immunofluorescent intensity increased but localization was unaltered by 2SW conditions. In support of our hypothesis, cldn-10/Cldn-10 TJ protein dynamics in gill and OE of mummichogs and TJ localization are functionally consistent with the creation and maintenance of salinity-responsive, cation-selective pores that facilitate Na+ secretion in hyperosmotic environments.http://bio.biologists.org/content/10/7/bio058868osmoregulationtight junctionfundulusionocyteaccessory cellcftr |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Chun Chih Chen William S. Marshall George N. Robertson Regina R. F. Cozzi Scott P. Kelly |
spellingShingle |
Chun Chih Chen William S. Marshall George N. Robertson Regina R. F. Cozzi Scott P. Kelly Mummichog gill and operculum exhibit functionally consistent claudin-10 paralog profiles and Claudin-10c hypersaline response Biology Open osmoregulation tight junction fundulus ionocyte accessory cell cftr |
author_facet |
Chun Chih Chen William S. Marshall George N. Robertson Regina R. F. Cozzi Scott P. Kelly |
author_sort |
Chun Chih Chen |
title |
Mummichog gill and operculum exhibit functionally consistent claudin-10 paralog profiles and Claudin-10c hypersaline response |
title_short |
Mummichog gill and operculum exhibit functionally consistent claudin-10 paralog profiles and Claudin-10c hypersaline response |
title_full |
Mummichog gill and operculum exhibit functionally consistent claudin-10 paralog profiles and Claudin-10c hypersaline response |
title_fullStr |
Mummichog gill and operculum exhibit functionally consistent claudin-10 paralog profiles and Claudin-10c hypersaline response |
title_full_unstemmed |
Mummichog gill and operculum exhibit functionally consistent claudin-10 paralog profiles and Claudin-10c hypersaline response |
title_sort |
mummichog gill and operculum exhibit functionally consistent claudin-10 paralog profiles and claudin-10c hypersaline response |
publisher |
The Company of Biologists |
series |
Biology Open |
issn |
2046-6390 |
publishDate |
2021-07-01 |
description |
Claudin (Cldn)-10 tight junction (TJ) proteins are hypothesized to form the paracellular Na+ secretion pathway of hyposmoregulating mummichog (Fundulus heteroclitus) branchial epithelia. Organ-specific expression profiles showed that only branchial organs [the gill and opercular epithelium (OE)] exhibited abundant cldn-10 paralog transcripts, which typically increased following seawater (SW) to hypersaline (2SW) challenge. Post-translational properties, protein abundance, and ionocyte localization of Cldn-10c, were then examined in gill and OE. Western blot analysis revealed two Cldn-10c immunoreactive bands in the mummichog gill and OE at ∼29 kDa and ∼40 kDa. The heavier protein could be eliminated by glycosidase treatment, demonstrating the novel presence of a glycosylated Cldn-10c. Protein abundance of Cldn-10c increased in gill and OE of 2SW-exposed fish. Cldn-10c localized to the sides of gill and OE ionocyte apical crypts and partially colocalized with cystic fibrosis transmembrane conductance regulator and F-actin, consistent with TJ complex localization. Cldn-10c immunofluorescent intensity increased but localization was unaltered by 2SW conditions. In support of our hypothesis, cldn-10/Cldn-10 TJ protein dynamics in gill and OE of mummichogs and TJ localization are functionally consistent with the creation and maintenance of salinity-responsive, cation-selective pores that facilitate Na+ secretion in hyperosmotic environments. |
topic |
osmoregulation tight junction fundulus ionocyte accessory cell cftr |
url |
http://bio.biologists.org/content/10/7/bio058868 |
work_keys_str_mv |
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