Association of SOD2 p.V16A polymorphism with Parkinson’s disease: A meta-analysis in Han Chinese

• Main Authors: Yih-Ru Wu, Kuo-Hsuan Chang, Chih-Ying Chao, Chih-Hsin Lin, Yi-Chun Chen, Tsai-Wei Liu, Guey-Jen Lee-Chen, Chiung-Mei Chen
• Format: Article
• Language: English
• Published: Elsevier 2021-01-01
• Series: Journal of the Formosan Medical Association
• Subjects: Meta-analysis; Parkinson's disease; SOD2 p.V16A
• Online Access: http://www.sciencedirect.com/science/article/pii/S0929664620302837

Background: Oxidative stress could participate in the pathogenesis of Parkinson's disease (PD). However, the role of genetic variation of superoxide dismutase 2 (SOD2), an important regulator against oxidative stress, in PD remains to be elucidated. Methods: We screened SOD2 gene variation by sequencing cDNA from 72 patients with early onset PD. A cohort of PD (n = 609) and ethnically matched controls (n = 681) were further examined for the identified sequence variant by PCR and NaeI restriction analysis. Results: Only a reported c.47T>C polymorphism (rs4880, SOD2 p.V16A) was found by cDNA sequencing. Case-control study of c.47T>C revealed that genotype and allele frequencies were in Hardy–Weinberg equilibrium in both patients and healthy controls. In a recessive model, those with CC genotype had a 2.61-fold increased risk of PD (95% CI: 1.08–6.30, P = 0.03) compared to subjects with TT and TC genotypes. Significant association between CC genotype and PD in non-smokers was also observed after stratification according to the history of smoking (3.54-fold increased risk of PD, 95% CI: 1.17–10.72, P = 0.02). Meta-analysis by combining studies of Chinese in China, Singapore, and Taiwan (total 2302 cases and 2029 controls) consistently showed CC genotype with increased risk of PD (OR = 1.77, 95% CI: 1.15–2.71, P = 0.01). Conclusion: Our findings demonstrate that SOD2 p.V16A may play a role in the susceptibility of PD in Han Chinese.