Immunopathological Aspects of Experimental Trypanosoma cruzi Reinfections
Chagas disease is caused by Trypanosoma cruzi infection. Besides the host-related factors, such as immune response and genetic background, the parasite, strain, and occurrences of reinfection episodes, may influence disease outcome. Our results demonstrate that both the primary infection and the rei...
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doaj-2baa6e5b6b7c4b45a51ba93965d15f632020-11-25T00:52:20ZengHindawi LimitedBioMed Research International2314-61332314-61412014-01-01201410.1155/2014/648715648715Immunopathological Aspects of Experimental Trypanosoma cruzi ReinfectionsJuliana Reis Machado0Marcos Vinícius Silva1Diego Costa Borges2Crislaine Aparecida da Silva3Luis Eduardo Ramirez4Marlene Antônia dos Reis5Lúcio Roberto Castellano6Virmondes Rodrigues7Denise Bertulucci Rocha Rodrigues8Laboratory of Immunology, Federal University of Triângulo Mineiro, Cefores, Frei Paulino Street, 30, 38025-180 Uberaba, MG, BrazilLaboratory of Immunology, Federal University of Triângulo Mineiro, Cefores, Frei Paulino Street, 30, 38025-180 Uberaba, MG, BrazilDiscipline of Cell Biology, Federal University of Triângulo Mineiro, Frei Paulino Street, 30, 38025-180 Uberaba, MG, BrazilLaboratory of Immunology, Federal University of Triângulo Mineiro, Cefores, Frei Paulino Street, 30, 38025-180 Uberaba, MG, BrazilDiscipline of Parasitology, Federal University of Triângulo Mineiro, Frei Paulino Street, 30, 38025-180 Uberaba, MG, BrazilDiscipline of Pathology, Federal University of Triângulo Mineiro, Frei Paulino Street, 30, 38025-180 Uberaba, MG, BrazilTechnical School of Health, Federal University of Paraíba, 58051-900 João Pessoa, PB, BrazilLaboratory of Immunology, Federal University of Triângulo Mineiro, Cefores, Frei Paulino Street, 30, 38025-180 Uberaba, MG, BrazilLaboratory of Immunology, Federal University of Triângulo Mineiro, Cefores, Frei Paulino Street, 30, 38025-180 Uberaba, MG, BrazilChagas disease is caused by Trypanosoma cruzi infection. Besides the host-related factors, such as immune response and genetic background, the parasite, strain, and occurrences of reinfection episodes, may influence disease outcome. Our results demonstrate that both the primary infection and the reinfection with the Colombiana strain are connected with lower survival rate of the mice. After reinfection, parasitaemia is approximately ten times lower than in primary infected animals. Only Colombiana, Colombiana/Colombiana, and Y/Colombiana groups presented amastigote nests in cardiac tissue. Moreover, the mice infected and/or reinfected with the Colombiana strain had more T. cruzi nests, more intense inflammatory infiltrate, and higher in situ expression of TNF-α and IFN-γ than Y strain. Antigen-stimulated spleen cells from infected and/or reinfected animals produced higher levels of TNF-α, IFN-γ, and IL-10. Our results reinforce the idea that Chagas disease outcome is influenced by the strain of the infective parasite, being differentially modulated during reinfection episodes. It highlights the need of control strategies involving parasite strain characterization in endemic areas for Chagas disease.http://dx.doi.org/10.1155/2014/648715 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Juliana Reis Machado Marcos Vinícius Silva Diego Costa Borges Crislaine Aparecida da Silva Luis Eduardo Ramirez Marlene Antônia dos Reis Lúcio Roberto Castellano Virmondes Rodrigues Denise Bertulucci Rocha Rodrigues |
spellingShingle |
Juliana Reis Machado Marcos Vinícius Silva Diego Costa Borges Crislaine Aparecida da Silva Luis Eduardo Ramirez Marlene Antônia dos Reis Lúcio Roberto Castellano Virmondes Rodrigues Denise Bertulucci Rocha Rodrigues Immunopathological Aspects of Experimental Trypanosoma cruzi Reinfections BioMed Research International |
author_facet |
Juliana Reis Machado Marcos Vinícius Silva Diego Costa Borges Crislaine Aparecida da Silva Luis Eduardo Ramirez Marlene Antônia dos Reis Lúcio Roberto Castellano Virmondes Rodrigues Denise Bertulucci Rocha Rodrigues |
author_sort |
Juliana Reis Machado |
title |
Immunopathological Aspects of Experimental Trypanosoma cruzi Reinfections |
title_short |
Immunopathological Aspects of Experimental Trypanosoma cruzi Reinfections |
title_full |
Immunopathological Aspects of Experimental Trypanosoma cruzi Reinfections |
title_fullStr |
Immunopathological Aspects of Experimental Trypanosoma cruzi Reinfections |
title_full_unstemmed |
Immunopathological Aspects of Experimental Trypanosoma cruzi Reinfections |
title_sort |
immunopathological aspects of experimental trypanosoma cruzi reinfections |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2014-01-01 |
description |
Chagas disease is caused by Trypanosoma cruzi infection. Besides the host-related factors, such as immune response and genetic background, the parasite, strain, and occurrences of reinfection episodes, may influence disease outcome. Our results demonstrate that both the primary infection and the reinfection with the Colombiana strain are connected with lower survival rate of the mice. After reinfection, parasitaemia is approximately ten times lower than in primary infected animals. Only Colombiana, Colombiana/Colombiana, and Y/Colombiana groups presented amastigote nests in cardiac tissue. Moreover, the mice infected and/or reinfected with the Colombiana strain had more T. cruzi nests, more intense inflammatory infiltrate, and higher in situ expression of TNF-α and IFN-γ than Y strain. Antigen-stimulated spleen cells from infected and/or reinfected animals produced higher levels of TNF-α, IFN-γ, and IL-10. Our results reinforce the idea that Chagas disease outcome is influenced by the strain of the infective parasite, being differentially modulated during reinfection episodes. It highlights the need of control strategies involving parasite strain characterization in endemic areas for Chagas disease. |
url |
http://dx.doi.org/10.1155/2014/648715 |
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