Immunochromatographic strip for ultrasensitive detection of fumonisin B1

• Main Authors: Kai Hao, Steven Suryoprabowo, Tao Hong, Shanshan Song, Liqiang Liu, Qiankun Zheng, Hua Kuang
• Format: Article
• Language: English
• Published: Taylor & Francis Group 2018-01-01
• Series: Food and Agricultural Immunology
• Subjects: fumonisins; fumonisin b1; immunochromatographic assay; monoclonal antibody; strip test
• Online Access: http://dx.doi.org/10.1080/09540105.2018.1439455

A sensitive monoclonal antibody (mAb) (1H1) against fumonisin B1 (FB1) was produced in our laboratory. A rapid, simple, and an immunochromatographic strip has been developed for the ultrasensitive detection of FB1 in corn samples. Under the optimized condition, the cut-off limits of test strips for FB1 were found to be 50 ng/mL in 0.01 M PBS and 25 ng/mL in corn samples. Results could be obtained within 5 min. The results revealed that the developed method is a rapid, sensitive, and simple tool for the detection of FB1.