Frequency of Resistance and Susceptible Bacteria Isolated from Houseflies
Background: In this study, we determine the vector competence of Musca domestica with reference to the transmission of susceptible and resistance bacterial strains in hospitals and slaughter house in Sanandaj City, west Iran. Methods: Totally 908 houseflies were collected to isolate bacteria from t...
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Tehran University of Medical Sciences
2010-12-01
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doaj-2bacfeaccd3445fbb240b364d508f14c2020-11-25T03:02:52ZengTehran University of Medical SciencesJournal of Arthropod-Borne Diseases 1735-71792322-22712010-12-014271Frequency of Resistance and Susceptible Bacteria Isolated from HousefliesB Davari0E Kalantar1A Zahirnia2SH Moosa-Kazemi3Department of Parasitology, School of Medicine, Medical University of Kurdistan, Sananadaj, IranLiver and Digestive Research Center, Tohid Hospital, Medical University of Kurdistan, Sananadaj, IranDepartment of Parasitology, School of Medicine, Medical University of Hamedan, Hamedan, IranDepartment of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, IranBackground: In this study, we determine the vector competence of Musca domestica with reference to the transmission of susceptible and resistance bacterial strains in hospitals and slaughter house in Sanandaj City, west Iran. Methods: Totally 908 houseflies were collected to isolate bacteria from their external body based on standard procedures.Antibiotic susceptibility testing was performed by Kirby-Bauer disc diffusion method on Mueller Hinton agar based on recommendations of CLSI (formerly the National Committee for Clinical Laboratory Standards). Results: From collected houseflies, 366 bacteria species were isolated. The most common isolated bacterium at hospitals was Klebsiella pneumoniae 43.3% (n= 90) followed by Pseudomonas aeruginosa 37% (n= 77), while that of slaughterhouse was Proteus mirabilis. 29.1% (n= 46) followed by Citrobacter freundii 28.4% (n= 45). Among all the isolates from hospitals, cephalexin, chloramphenicol, ampicillin, and tetracycline, resistance rates were above 32.5% and gentamicin expressed the highest susceptibility among all the isolates from hospitals. It is worth to note that K. pneumoniae showed 61% and 44.5% resistance to cephalexin and chloramphenicol respectively. Similarly, all isolates from slaughterhouse were more than 28% and 30% resistant to cephalexin and chloramphenicol respectively. Surprisingly, among all the isolates, Citrobacter freundii were highly resistant to gentamicin. Conclusion: Houseflies collected from hospitals and slaughterhouse may be involved in the spread of drug resistant bacteria and may increase the potential of human exposure to drug resistant bacteria. https://jad.tums.ac.ir/index.php/jad/article/view/73House flybacteriumantibacterial resistancehospitalsslaughterhouse |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
B Davari E Kalantar A Zahirnia SH Moosa-Kazemi |
spellingShingle |
B Davari E Kalantar A Zahirnia SH Moosa-Kazemi Frequency of Resistance and Susceptible Bacteria Isolated from Houseflies Journal of Arthropod-Borne Diseases House fly bacterium antibacterial resistance hospitals slaughterhouse |
author_facet |
B Davari E Kalantar A Zahirnia SH Moosa-Kazemi |
author_sort |
B Davari |
title |
Frequency of Resistance and Susceptible Bacteria Isolated from Houseflies |
title_short |
Frequency of Resistance and Susceptible Bacteria Isolated from Houseflies |
title_full |
Frequency of Resistance and Susceptible Bacteria Isolated from Houseflies |
title_fullStr |
Frequency of Resistance and Susceptible Bacteria Isolated from Houseflies |
title_full_unstemmed |
Frequency of Resistance and Susceptible Bacteria Isolated from Houseflies |
title_sort |
frequency of resistance and susceptible bacteria isolated from houseflies |
publisher |
Tehran University of Medical Sciences |
series |
Journal of Arthropod-Borne Diseases |
issn |
1735-7179 2322-2271 |
publishDate |
2010-12-01 |
description |
Background: In this study, we determine the vector competence of Musca domestica with reference to the transmission of susceptible and resistance bacterial strains in hospitals and slaughter house in Sanandaj City, west Iran.
Methods: Totally 908 houseflies were collected to isolate bacteria from their external body based on standard procedures.Antibiotic susceptibility testing was performed by Kirby-Bauer disc diffusion method on Mueller Hinton agar based on recommendations of CLSI (formerly the National Committee for Clinical Laboratory Standards).
Results: From collected houseflies, 366 bacteria species were isolated. The most common isolated bacterium at hospitals was Klebsiella pneumoniae 43.3% (n= 90) followed by Pseudomonas aeruginosa 37% (n= 77), while that of slaughterhouse was Proteus mirabilis. 29.1% (n= 46) followed by Citrobacter freundii 28.4% (n= 45). Among all the isolates from hospitals, cephalexin, chloramphenicol, ampicillin, and tetracycline, resistance rates were above 32.5% and gentamicin expressed the highest susceptibility among all the isolates from hospitals. It is worth to note that K. pneumoniae showed 61% and 44.5% resistance to cephalexin and chloramphenicol respectively. Similarly, all isolates from slaughterhouse were more than 28% and 30% resistant to cephalexin and chloramphenicol respectively. Surprisingly, among all the isolates, Citrobacter freundii were highly resistant to gentamicin.
Conclusion: Houseflies collected from hospitals and slaughterhouse may be involved in the spread of drug resistant bacteria and may increase the potential of human exposure to drug resistant bacteria.
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topic |
House fly bacterium antibacterial resistance hospitals slaughterhouse |
url |
https://jad.tums.ac.ir/index.php/jad/article/view/73 |
work_keys_str_mv |
AT bdavari frequencyofresistanceandsusceptiblebacteriaisolatedfromhouseflies AT ekalantar frequencyofresistanceandsusceptiblebacteriaisolatedfromhouseflies AT azahirnia frequencyofresistanceandsusceptiblebacteriaisolatedfromhouseflies AT shmoosakazemi frequencyofresistanceandsusceptiblebacteriaisolatedfromhouseflies |
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1724687948025167872 |