Isolation and enzyme bioprospection of bacteria associated to Bruguiera cylindrica, a mangrove plant of North Sumatra, Indonesia

• Main Authors: Jendri Mamangkey, Dwi Suryanto, Erman Munir, Apon Zaenal Mustopa, Mada Triandala Sibero, Lucas William Mendes, Adrian Hartanto, Steven Taniwan, Maria Julissa Ek-Ramos, Arman Harahap, Amit Verma, Edy Trihatmoko, Wendry Setiyadi Putranto, Lukas Pardosi, La Ode Adi Parman Rudia
• Format: Article
• Language: English
• Published: Elsevier 2021-06-01
• Series: Biotechnology Reports
• Subjects: Bruguiera cylindrica; Extracellular enzymes; Mangrove; Protease; Vibrio alginolyticus
• Online Access: http://www.sciencedirect.com/science/article/pii/S2215017X21000333

Mangrove-associated bacteria are of industrial interest due to their diverse and versatile enzyme properties. This study investigates the culturable bacteria from a wide range of habitat in a Bruguiera cylindrica mangrove ecosystem in North Sumatra. Screening of extracellular hydrolytic enzymes showed multiple potential traits in amylase, cellulase, chitinase, phosphatase, protease, and urease production by bacterial isolates. Molecular identification based on 16S rDNA region of a potential strain, Vibrio alginolyticus Jme3−20 is then reported as a newly proteolytic agent. The strain also showed a stable growth under salinity (NaCl) stress with considerable phosphate solubilization activities. Protease activity was enhanced by optimizing the 0.5 % (w/v) sucrose and soy peptone in the fermentation medium. SDS-PAGE and zymogram analysis showed the presence of a 35-kDa MW protease. Hence, our study revealed important insights into the bacterial diversity and activity in mangrove ecosystems, evidencing the importance of microbial exploration in this ecosystem.