Novel Implant Coating Agent Promotes Gene Expression of Osteogenic Markers in Rats during Early Osseointegration
The aim of this study was to evaluate the early bone response around laminin-1-coated titanium implants. Forty-five rats distributed in three equally sized groups were provided with one control (turned) and one test (laminin-1-coated) implant and were sacrificed after 3, 7, and 21 days. Real-time re...
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Series: | International Journal of Biomaterials |
Online Access: | http://dx.doi.org/10.1155/2012/579274 |
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doaj-2bbdde52994945a9874e96d2a21e96c22020-11-24T22:32:44ZengHindawi LimitedInternational Journal of Biomaterials1687-87871687-87952012-01-01201210.1155/2012/579274579274Novel Implant Coating Agent Promotes Gene Expression of Osteogenic Markers in Rats during Early OsseointegrationKostas Bougas0Ryo Jimbo1Ying Xue2Kamal Mustafa3Ann Wennerberg4Department of Prosthodontics, Faculty of Odontology, Malmö University, 205 06 Malmö, SwedenDepartment of Prosthodontics, Faculty of Odontology, Malmö University, 205 06 Malmö, SwedenDepartment of Clinical Dentistry, Center for Clinical Research, Faculty of Medicine and Dentistry, University of Bergen, Årstadveien 17, 5009 Bergen, NorwayDepartment of Clinical Dentistry, Center for Clinical Research, Faculty of Medicine and Dentistry, University of Bergen, Årstadveien 17, 5009 Bergen, NorwayDepartment of Prosthodontics, Faculty of Odontology, Malmö University, 205 06 Malmö, SwedenThe aim of this study was to evaluate the early bone response around laminin-1-coated titanium implants. Forty-five rats distributed in three equally sized groups were provided with one control (turned) and one test (laminin-1-coated) implant and were sacrificed after 3, 7, and 21 days. Real-time reverse-transcriptase polymerase chain reaction was performed for osteoblast markers (alkaline phosphatase, runt-related transcription factor 2, osteocalcin, type I collagen, and bone morphogenic protein 2), osteoclast markers (cathepsin K and tartrate-resistant acid phosphatase), inflammation markers (tumor necrosis factor α, interleukin 1β and interleukin 10), and integrin β1. Bone implant contact (BIC) and bone area (BA) were assessed and compared to the gene expression. After 3 days, the expression of bone markers was higher for the control group. After 7 days, the expression of integrin β1 and osteogenic markers was enhanced for the test group, while cathepsin K and inflammation markers were down-regulated. No significant differences in BIC or BA were detected between test and control at any time point. As a conclusion, implant coating with laminin-1 altered gene expression in the bone-implant interface. However, traditional evaluation methods, as histomorphometry, were not adequately sensitive to detect such changes due to the short follow-up time.http://dx.doi.org/10.1155/2012/579274 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kostas Bougas Ryo Jimbo Ying Xue Kamal Mustafa Ann Wennerberg |
spellingShingle |
Kostas Bougas Ryo Jimbo Ying Xue Kamal Mustafa Ann Wennerberg Novel Implant Coating Agent Promotes Gene Expression of Osteogenic Markers in Rats during Early Osseointegration International Journal of Biomaterials |
author_facet |
Kostas Bougas Ryo Jimbo Ying Xue Kamal Mustafa Ann Wennerberg |
author_sort |
Kostas Bougas |
title |
Novel Implant Coating Agent Promotes Gene Expression of Osteogenic Markers in Rats during Early Osseointegration |
title_short |
Novel Implant Coating Agent Promotes Gene Expression of Osteogenic Markers in Rats during Early Osseointegration |
title_full |
Novel Implant Coating Agent Promotes Gene Expression of Osteogenic Markers in Rats during Early Osseointegration |
title_fullStr |
Novel Implant Coating Agent Promotes Gene Expression of Osteogenic Markers in Rats during Early Osseointegration |
title_full_unstemmed |
Novel Implant Coating Agent Promotes Gene Expression of Osteogenic Markers in Rats during Early Osseointegration |
title_sort |
novel implant coating agent promotes gene expression of osteogenic markers in rats during early osseointegration |
publisher |
Hindawi Limited |
series |
International Journal of Biomaterials |
issn |
1687-8787 1687-8795 |
publishDate |
2012-01-01 |
description |
The aim of this study was to evaluate the early bone response around laminin-1-coated titanium implants. Forty-five rats distributed in three equally sized groups were provided with one control (turned) and one test (laminin-1-coated) implant and were sacrificed after 3, 7, and 21 days. Real-time reverse-transcriptase polymerase chain reaction was performed for osteoblast markers (alkaline phosphatase, runt-related transcription factor 2, osteocalcin, type I collagen, and bone morphogenic protein 2), osteoclast markers (cathepsin K and tartrate-resistant acid phosphatase), inflammation markers (tumor necrosis factor α, interleukin 1β and interleukin 10), and integrin β1. Bone implant contact (BIC) and bone area (BA) were assessed and compared to the gene expression. After 3 days, the expression of bone markers was higher for the control group. After 7 days, the expression of integrin β1 and osteogenic markers was enhanced for the test group, while cathepsin K and inflammation markers were down-regulated. No significant differences in BIC or BA were detected between test and control at any time point. As a conclusion, implant coating with laminin-1 altered gene expression in the bone-implant interface. However, traditional evaluation methods, as histomorphometry, were not adequately sensitive to detect such changes due to the short follow-up time. |
url |
http://dx.doi.org/10.1155/2012/579274 |
work_keys_str_mv |
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