In Vitro and In Vivo Anticancer Effects of Sterol Fraction from Red Algae Porphyra dentata
Porphyra dentata, an edible red macroalgae, is used as a folk medicine in Asia. This study evaluated in vitro and in vivo the protective effect of a sterol fraction from P. dentata against breast cancer linked to tumor-induced myeloid derived-suppressor cells (MDSCs). A sterol fraction containing ch...
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Online Access: | http://dx.doi.org/10.1155/2013/493869 |
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doaj-2bc2d9fe1f224da1b7cf5d7365e966842020-11-24T23:41:42ZengHindawi LimitedEvidence-Based Complementary and Alternative Medicine1741-427X1741-42882013-01-01201310.1155/2013/493869493869In Vitro and In Vivo Anticancer Effects of Sterol Fraction from Red Algae Porphyra dentataKatarzyna Kazłowska0Hong-Ting Victor Lin1Shun-Hsien Chang2Guo-Jane Tsai3Department of Food Science, National Taiwan Ocean University, 2 Pei-Ning Road, Keelung 20224, TaiwanDepartment of Food Science, National Taiwan Ocean University, 2 Pei-Ning Road, Keelung 20224, TaiwanDepartment of Food Science, National Taiwan Ocean University, 2 Pei-Ning Road, Keelung 20224, TaiwanDepartment of Food Science, National Taiwan Ocean University, 2 Pei-Ning Road, Keelung 20224, TaiwanPorphyra dentata, an edible red macroalgae, is used as a folk medicine in Asia. This study evaluated in vitro and in vivo the protective effect of a sterol fraction from P. dentata against breast cancer linked to tumor-induced myeloid derived-suppressor cells (MDSCs). A sterol fraction containing cholesterol, β-sitosterol, and campesterol was prepared by solvent fractionation of methanol extract of P. dentata in silica gel column chromatography. This sterol fraction in vitro significantly inhibited cell growth and induced apoptosis in 4T1 cancer cells. Intraperitoneal injection of this sterol fraction at 10 and 25 mg/kg body weight into 4T1 cell-implanted tumor BALB/c mice significantly inhibited the growth of tumor nodules and increased the survival rate of mice. This sterol fraction significantly decreased the reactive oxygen species (ROS) and arginase activity of MDSCs in tumor-bearing mice. Therefore, the sterol fraction from P. dentata showed potential for protecting an organism from 4T1 cell-based tumor genesis.http://dx.doi.org/10.1155/2013/493869 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Katarzyna Kazłowska Hong-Ting Victor Lin Shun-Hsien Chang Guo-Jane Tsai |
spellingShingle |
Katarzyna Kazłowska Hong-Ting Victor Lin Shun-Hsien Chang Guo-Jane Tsai In Vitro and In Vivo Anticancer Effects of Sterol Fraction from Red Algae Porphyra dentata Evidence-Based Complementary and Alternative Medicine |
author_facet |
Katarzyna Kazłowska Hong-Ting Victor Lin Shun-Hsien Chang Guo-Jane Tsai |
author_sort |
Katarzyna Kazłowska |
title |
In Vitro and In Vivo Anticancer Effects of Sterol Fraction from Red Algae Porphyra dentata |
title_short |
In Vitro and In Vivo Anticancer Effects of Sterol Fraction from Red Algae Porphyra dentata |
title_full |
In Vitro and In Vivo Anticancer Effects of Sterol Fraction from Red Algae Porphyra dentata |
title_fullStr |
In Vitro and In Vivo Anticancer Effects of Sterol Fraction from Red Algae Porphyra dentata |
title_full_unstemmed |
In Vitro and In Vivo Anticancer Effects of Sterol Fraction from Red Algae Porphyra dentata |
title_sort |
in vitro and in vivo anticancer effects of sterol fraction from red algae porphyra dentata |
publisher |
Hindawi Limited |
series |
Evidence-Based Complementary and Alternative Medicine |
issn |
1741-427X 1741-4288 |
publishDate |
2013-01-01 |
description |
Porphyra dentata, an edible red macroalgae, is used as a folk medicine in Asia. This study evaluated in vitro and in vivo the protective effect of a sterol fraction from P. dentata against breast cancer linked to tumor-induced myeloid derived-suppressor cells (MDSCs). A sterol fraction containing cholesterol, β-sitosterol, and campesterol was prepared by solvent fractionation of methanol extract of P. dentata in silica gel column chromatography. This sterol fraction in vitro significantly inhibited cell growth and induced apoptosis in 4T1 cancer cells. Intraperitoneal injection of this sterol fraction at 10 and 25 mg/kg body weight into 4T1 cell-implanted tumor BALB/c mice significantly inhibited the growth of tumor nodules and increased the survival rate of mice. This sterol fraction significantly decreased the reactive oxygen species (ROS) and arginase activity of MDSCs in tumor-bearing mice. Therefore, the sterol fraction from P. dentata showed potential for protecting an organism from 4T1 cell-based tumor genesis. |
url |
http://dx.doi.org/10.1155/2013/493869 |
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