Devitalizing Effect of High Hydrostatic Pressure on Human Cells—Influence on Cell Death in Osteoblasts and Chondrocytes
Chemical and physical processing of allografts is associated with a significant reduction in biomechanics. Therefore, treatment of tissue with high hydrostatic pressure (HHP) offers the possibility to devitalize tissue gently without changing biomechanical properties. To obtain an initial assessment...
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doaj-2bc356e5dba240c4a50809a5a96a379d2020-11-25T03:17:49ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-05-01213836383610.3390/ijms21113836Devitalizing Effect of High Hydrostatic Pressure on Human Cells—Influence on Cell Death in Osteoblasts and ChondrocytesJanine Waletzko0Michael Dau1Anika Seyfarth2Armin Springer3Marcus Frank4Rainer Bader5Anika Jonitz-Heincke6Department of Oral, Maxillofacial and Plastic Surgery, University Medical Center Rostock, 18057 Rostock, GermanyDepartment of Oral, Maxillofacial and Plastic Surgery, University Medical Center Rostock, 18057 Rostock, GermanyDepartment of Orthopedics, Biomechanics and Implant Technology Research Laboratory, University Medical Center Rostock, 18057 Rostock, GermanyMedical Biology and Electron Microscopy Center, University Medical Center Rostock, 18057 Rostock, GermanyMedical Biology and Electron Microscopy Center, University Medical Center Rostock, 18057 Rostock, GermanyDepartment of Orthopedics, Biomechanics and Implant Technology Research Laboratory, University Medical Center Rostock, 18057 Rostock, GermanyDepartment of Orthopedics, Biomechanics and Implant Technology Research Laboratory, University Medical Center Rostock, 18057 Rostock, GermanyChemical and physical processing of allografts is associated with a significant reduction in biomechanics. Therefore, treatment of tissue with high hydrostatic pressure (HHP) offers the possibility to devitalize tissue gently without changing biomechanical properties. To obtain an initial assessment of the effectiveness of HHP treatment, human osteoblasts and chondrocytes were treated with different HHPs (100–150 MPa, 250–300 MPa, 450–500 MPa). Devitalization efficiency was determined by analyzing the metabolic activity via WST-1(water-soluble tetrazolium salt) assay. The type of cell death was detected with an apoptosis/necrosis ELISA (enzyme-linked immune sorbent assay) and flow cytometry. Field emission scanning electron microscopy (FESEM) and transmission electron microscopy (TEM) were carried out to detect the degree of cell destruction. After HHP treatment, the metabolic activities of both cell types decreased, whereas HHP of 250 MPa and higher resulted in metabolic inactivation. Further, the highest HHP range induced mostly necrosis while the lower HHP ranges induced apoptosis and necrosis equally. FESEM and TEM analyses of treated osteoblasts revealed pressure-dependent cell damage. In the present study, it could be proven that a pressure range of 250–300 MPa can be used for cell devitalization. However, in order to treat bone and cartilage tissue gently with HHP, the results of our cell experiments must be verified for tissue samples in future studies.https://www.mdpi.com/1422-0067/21/11/3836high hydrostatic pressuredevitalizationdecellularizationallograftsregenerative medicinebone and cartilage regeneration |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Janine Waletzko Michael Dau Anika Seyfarth Armin Springer Marcus Frank Rainer Bader Anika Jonitz-Heincke |
spellingShingle |
Janine Waletzko Michael Dau Anika Seyfarth Armin Springer Marcus Frank Rainer Bader Anika Jonitz-Heincke Devitalizing Effect of High Hydrostatic Pressure on Human Cells—Influence on Cell Death in Osteoblasts and Chondrocytes International Journal of Molecular Sciences high hydrostatic pressure devitalization decellularization allografts regenerative medicine bone and cartilage regeneration |
author_facet |
Janine Waletzko Michael Dau Anika Seyfarth Armin Springer Marcus Frank Rainer Bader Anika Jonitz-Heincke |
author_sort |
Janine Waletzko |
title |
Devitalizing Effect of High Hydrostatic Pressure on Human Cells—Influence on Cell Death in Osteoblasts and Chondrocytes |
title_short |
Devitalizing Effect of High Hydrostatic Pressure on Human Cells—Influence on Cell Death in Osteoblasts and Chondrocytes |
title_full |
Devitalizing Effect of High Hydrostatic Pressure on Human Cells—Influence on Cell Death in Osteoblasts and Chondrocytes |
title_fullStr |
Devitalizing Effect of High Hydrostatic Pressure on Human Cells—Influence on Cell Death in Osteoblasts and Chondrocytes |
title_full_unstemmed |
Devitalizing Effect of High Hydrostatic Pressure on Human Cells—Influence on Cell Death in Osteoblasts and Chondrocytes |
title_sort |
devitalizing effect of high hydrostatic pressure on human cells—influence on cell death in osteoblasts and chondrocytes |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1661-6596 1422-0067 |
publishDate |
2020-05-01 |
description |
Chemical and physical processing of allografts is associated with a significant reduction in biomechanics. Therefore, treatment of tissue with high hydrostatic pressure (HHP) offers the possibility to devitalize tissue gently without changing biomechanical properties. To obtain an initial assessment of the effectiveness of HHP treatment, human osteoblasts and chondrocytes were treated with different HHPs (100–150 MPa, 250–300 MPa, 450–500 MPa). Devitalization efficiency was determined by analyzing the metabolic activity via WST-1(water-soluble tetrazolium salt) assay. The type of cell death was detected with an apoptosis/necrosis ELISA (enzyme-linked immune sorbent assay) and flow cytometry. Field emission scanning electron microscopy (FESEM) and transmission electron microscopy (TEM) were carried out to detect the degree of cell destruction. After HHP treatment, the metabolic activities of both cell types decreased, whereas HHP of 250 MPa and higher resulted in metabolic inactivation. Further, the highest HHP range induced mostly necrosis while the lower HHP ranges induced apoptosis and necrosis equally. FESEM and TEM analyses of treated osteoblasts revealed pressure-dependent cell damage. In the present study, it could be proven that a pressure range of 250–300 MPa can be used for cell devitalization. However, in order to treat bone and cartilage tissue gently with HHP, the results of our cell experiments must be verified for tissue samples in future studies. |
topic |
high hydrostatic pressure devitalization decellularization allografts regenerative medicine bone and cartilage regeneration |
url |
https://www.mdpi.com/1422-0067/21/11/3836 |
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