Insulin-induced lipid binding to hemoglobin
Under hypoglycemic conditions, concomitant hyperinsulinism causes an apparent modification of hemoglobin (Hb) which is manifested by its aggregation (Niketić et al., Clin. Chim. Acta 197 (1991) 47). In the present work the causes and mechanisms underlying this Hb modification were studied. Hemoglobi...
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Serbian Chemical Society
2003-01-01
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doaj-2bc571bec8d94fe2a568430941ec0db32020-12-24T07:40:27ZengSerbian Chemical Society Journal of the Serbian Chemical Society0352-51391820-74212003-01-01681253410.2298/JSC0301025T0352-51390301025TInsulin-induced lipid binding to hemoglobinTomašević Nenad0Nikolić Milan R.1Klappe Karen2Hoekstra Dick3Niketić Vesna P.4Chemistry Department, University of Belgrade, Belgrade, YugoslaviaChemistry Department, University of Belgrade, YugoslaviaLaboratory of Physiological Chemistry, University of Groningen, Bloemsingel 10, 9712 KZ Groningen, The NetherlandsLaboratory of Physiological Chemistry, University of Groningen, Bloemsingel 10, 9712 KZ Groningen, The NetherlandsChemistry Department, University of Belgrade, Studentski trg 16, P. O. Box 158, YU-11001 Belgrade, YugoslaviaUnder hypoglycemic conditions, concomitant hyperinsulinism causes an apparent modification of hemoglobin (Hb) which is manifested by its aggregation (Niketić et al., Clin. Chim. Acta 197 (1991) 47). In the present work the causes and mechanisms underlying this Hb modification were studied. Hemoglobin isolated from normal erythrocytes incubated with insulin was analyzed by applying 31P-spectrometry and lipid extraction and analysis. To study the dynamics of the plasma membrane during hyperinsulinism, a fluorescent lipid-analog was applied. In the presence of insulin phosphatidylserine (PS), phosphatidylethanolamine (PE) and cholesterol were found to bind to Hb. Lipid binding resulted in Hb aggregation, a condition that can be reproduced when phospholipids are incubated with Hb in vitro. Using a fluorescent lipid-analog, it was also shown that exposing erythrocytes to supraphysiological concentrations of insulin in vitro resulted in the internalization of lipids. The results presented in this work may have relevance to cases of diabetes mellitus and hypoglycemia.http://www.doiserbia.nb.rs/img/doi/0352-5139/2003/0352-51390301025T.pdfinsulinerythrocytehypoglycemiahyperinsulinismmembrane lipidshemoglobin. |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Tomašević Nenad Nikolić Milan R. Klappe Karen Hoekstra Dick Niketić Vesna P. |
spellingShingle |
Tomašević Nenad Nikolić Milan R. Klappe Karen Hoekstra Dick Niketić Vesna P. Insulin-induced lipid binding to hemoglobin Journal of the Serbian Chemical Society insulin erythrocyte hypoglycemia hyperinsulinism membrane lipids hemoglobin. |
author_facet |
Tomašević Nenad Nikolić Milan R. Klappe Karen Hoekstra Dick Niketić Vesna P. |
author_sort |
Tomašević Nenad |
title |
Insulin-induced lipid binding to hemoglobin |
title_short |
Insulin-induced lipid binding to hemoglobin |
title_full |
Insulin-induced lipid binding to hemoglobin |
title_fullStr |
Insulin-induced lipid binding to hemoglobin |
title_full_unstemmed |
Insulin-induced lipid binding to hemoglobin |
title_sort |
insulin-induced lipid binding to hemoglobin |
publisher |
Serbian Chemical Society |
series |
Journal of the Serbian Chemical Society |
issn |
0352-5139 1820-7421 |
publishDate |
2003-01-01 |
description |
Under hypoglycemic conditions, concomitant hyperinsulinism causes an apparent modification of hemoglobin (Hb) which is manifested by its aggregation (Niketić et al., Clin. Chim. Acta 197 (1991) 47). In the present work the causes and mechanisms underlying this Hb modification were studied. Hemoglobin isolated from normal erythrocytes incubated with insulin was analyzed by applying 31P-spectrometry and lipid extraction and analysis. To study the dynamics of the plasma membrane during hyperinsulinism, a fluorescent lipid-analog was applied. In the presence of insulin phosphatidylserine (PS), phosphatidylethanolamine (PE) and cholesterol were found to bind to Hb. Lipid binding resulted in Hb aggregation, a condition that can be reproduced when phospholipids are incubated with Hb in vitro. Using a fluorescent lipid-analog, it was also shown that exposing erythrocytes to supraphysiological concentrations of insulin in vitro resulted in the internalization of lipids. The results presented in this work may have relevance to cases of diabetes mellitus and hypoglycemia. |
topic |
insulin erythrocyte hypoglycemia hyperinsulinism membrane lipids hemoglobin. |
url |
http://www.doiserbia.nb.rs/img/doi/0352-5139/2003/0352-51390301025T.pdf |
work_keys_str_mv |
AT tomasevicnenad insulininducedlipidbindingtohemoglobin AT nikolicmilanr insulininducedlipidbindingtohemoglobin AT klappekaren insulininducedlipidbindingtohemoglobin AT hoekstradick insulininducedlipidbindingtohemoglobin AT niketicvesnap insulininducedlipidbindingtohemoglobin |
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