A Cost-Efficient Multiwavelength LED-Based System for Quantitative Photoacoustic Measurements
The unique ability of photoacoustic (PA) sensing to provide optical absorption information of biomolecules deep inside turbid tissues with high sensitivity has recently enabled the development of various novel diagnostic systems for biomedical applications. In many cases, PA setups can be bulky, com...
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doaj-2bca4736021d4f589b1056999bda8e952021-07-23T14:06:07ZengMDPI AGSensors1424-82202021-07-01214888488810.3390/s21144888A Cost-Efficient Multiwavelength LED-Based System for Quantitative Photoacoustic MeasurementsMichalis Orfanakis0George J. Tserevelakis1Giannis Zacharakis2Foundation for Research and Technology Hellas, Institute of Electronic Structure and Laser, N. Plastira 100, GR-70013 Heraklion, GreeceFoundation for Research and Technology Hellas, Institute of Electronic Structure and Laser, N. Plastira 100, GR-70013 Heraklion, GreeceFoundation for Research and Technology Hellas, Institute of Electronic Structure and Laser, N. Plastira 100, GR-70013 Heraklion, GreeceThe unique ability of photoacoustic (PA) sensing to provide optical absorption information of biomolecules deep inside turbid tissues with high sensitivity has recently enabled the development of various novel diagnostic systems for biomedical applications. In many cases, PA setups can be bulky, complex, and costly, as they typically require the integration of expensive Q-switched nanosecond lasers, and also presents limited wavelength availability. This article presents a compact, cost-efficient, multiwavelength PA sensing system for quantitative measurements, by utilizing two high-power LED sources emitting at central wavelengths of 444 and 628 nm, respectively, and a single-element ultrasonic transducer at 3.5 MHz for signal detection. We investigate the performance of LEDs in pulsed mode and explore the dependence of PA responses on absorber’s concentration and applied energy fluence using tissue-mimicking phantoms demonstrating both optical absorption and scattering properties. Finally, we apply the developed system on the spectral unmixing of two absorbers contained at various relative concentrations in the phantoms, to provide accurate estimations with absolute deviations ranging between 0.4 and 12.3%. An upgraded version of the PA system may provide valuable in-vivo multiparametric measurements of important biomarkers, such as hemoglobin oxygenation, melanin concentration, local lipid content, and glucose levels.https://www.mdpi.com/1424-8220/21/14/4888sensingphotoacousticsLEDunmixing |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Michalis Orfanakis George J. Tserevelakis Giannis Zacharakis |
spellingShingle |
Michalis Orfanakis George J. Tserevelakis Giannis Zacharakis A Cost-Efficient Multiwavelength LED-Based System for Quantitative Photoacoustic Measurements Sensors sensing photoacoustics LED unmixing |
author_facet |
Michalis Orfanakis George J. Tserevelakis Giannis Zacharakis |
author_sort |
Michalis Orfanakis |
title |
A Cost-Efficient Multiwavelength LED-Based System for Quantitative Photoacoustic Measurements |
title_short |
A Cost-Efficient Multiwavelength LED-Based System for Quantitative Photoacoustic Measurements |
title_full |
A Cost-Efficient Multiwavelength LED-Based System for Quantitative Photoacoustic Measurements |
title_fullStr |
A Cost-Efficient Multiwavelength LED-Based System for Quantitative Photoacoustic Measurements |
title_full_unstemmed |
A Cost-Efficient Multiwavelength LED-Based System for Quantitative Photoacoustic Measurements |
title_sort |
cost-efficient multiwavelength led-based system for quantitative photoacoustic measurements |
publisher |
MDPI AG |
series |
Sensors |
issn |
1424-8220 |
publishDate |
2021-07-01 |
description |
The unique ability of photoacoustic (PA) sensing to provide optical absorption information of biomolecules deep inside turbid tissues with high sensitivity has recently enabled the development of various novel diagnostic systems for biomedical applications. In many cases, PA setups can be bulky, complex, and costly, as they typically require the integration of expensive Q-switched nanosecond lasers, and also presents limited wavelength availability. This article presents a compact, cost-efficient, multiwavelength PA sensing system for quantitative measurements, by utilizing two high-power LED sources emitting at central wavelengths of 444 and 628 nm, respectively, and a single-element ultrasonic transducer at 3.5 MHz for signal detection. We investigate the performance of LEDs in pulsed mode and explore the dependence of PA responses on absorber’s concentration and applied energy fluence using tissue-mimicking phantoms demonstrating both optical absorption and scattering properties. Finally, we apply the developed system on the spectral unmixing of two absorbers contained at various relative concentrations in the phantoms, to provide accurate estimations with absolute deviations ranging between 0.4 and 12.3%. An upgraded version of the PA system may provide valuable in-vivo multiparametric measurements of important biomarkers, such as hemoglobin oxygenation, melanin concentration, local lipid content, and glucose levels. |
topic |
sensing photoacoustics LED unmixing |
url |
https://www.mdpi.com/1424-8220/21/14/4888 |
work_keys_str_mv |
AT michalisorfanakis acostefficientmultiwavelengthledbasedsystemforquantitativephotoacousticmeasurements AT georgejtserevelakis acostefficientmultiwavelengthledbasedsystemforquantitativephotoacousticmeasurements AT gianniszacharakis acostefficientmultiwavelengthledbasedsystemforquantitativephotoacousticmeasurements AT michalisorfanakis costefficientmultiwavelengthledbasedsystemforquantitativephotoacousticmeasurements AT georgejtserevelakis costefficientmultiwavelengthledbasedsystemforquantitativephotoacousticmeasurements AT gianniszacharakis costefficientmultiwavelengthledbasedsystemforquantitativephotoacousticmeasurements |
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1721285841950605312 |