Determination of urea content in urea cream by centrifugal partition chromatography

• Main Authors: Ying-Qun Wang, Shi-Sheng Wang, Ji Zhu, Lei Wang, Bo-Hai Jiang, Wei-Jie Zhao
• Format: Article
• Language: English
• Published: Elsevier 2016-04-01
• Series: Journal of Food and Drug Analysis
• Subjects: Centrifugal partition chromatography; Content determination; Urea cream
• Online Access: http://www.sciencedirect.com/science/article/pii/S1021949815001519

The objective of this study is to establish a centrifugal partition chromatography (CPC) method for determination of the urea ingredient in urea cream. The mechanism of this method is that urea is determined by UV detector at 430 nm after being extracted from the cream and derivatized on line via Ehrlich reaction in rotor of CPC, where the reaction products dissolve in the mobile phase and the cream matrix retains in the stationary phase. The mixed solvent consisting of n-hexane, methanol, hydrochloric acid and p-dimethylaminobenzaldehyde with a ratio of 1000 mL:1000 mL:18 mL:2.0 g is used for solvent system of CPC. The CPC method proposed offers good precision and convenience without complex sample pretreatment processes.