Determination of urea content in urea cream by centrifugal partition chromatography
The objective of this study is to establish a centrifugal partition chromatography (CPC) method for determination of the urea ingredient in urea cream. The mechanism of this method is that urea is determined by UV detector at 430 nm after being extracted from the cream and derivatized on line via Eh...
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doaj-2be8bb66600149c3b7325c3adf2045312020-11-24T23:52:08ZengElsevierJournal of Food and Drug Analysis1021-94982016-04-0124239940510.1016/j.jfda.2015.10.005Determination of urea content in urea cream by centrifugal partition chromatographyYing-Qun Wang0Shi-Sheng Wang1Ji Zhu2Lei Wang3Bo-Hai Jiang4Wei-Jie Zhao5School of Pharmaceutical Science and Technology, State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian, PR ChinaSchool of Pharmaceutical Science and Technology, State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian, PR ChinaSchool of Pharmaceutical Science and Technology, State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian, PR ChinaDalian Institute for Food and Drug Control, Dalian, PR ChinaDalian Institute for Food and Drug Control, Dalian, PR ChinaSchool of Pharmaceutical Science and Technology, State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian, PR ChinaThe objective of this study is to establish a centrifugal partition chromatography (CPC) method for determination of the urea ingredient in urea cream. The mechanism of this method is that urea is determined by UV detector at 430 nm after being extracted from the cream and derivatized on line via Ehrlich reaction in rotor of CPC, where the reaction products dissolve in the mobile phase and the cream matrix retains in the stationary phase. The mixed solvent consisting of n-hexane, methanol, hydrochloric acid and p-dimethylaminobenzaldehyde with a ratio of 1000 mL:1000 mL:18 mL:2.0 g is used for solvent system of CPC. The CPC method proposed offers good precision and convenience without complex sample pretreatment processes.http://www.sciencedirect.com/science/article/pii/S1021949815001519Centrifugal partition chromatographyContent determinationUrea cream |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ying-Qun Wang Shi-Sheng Wang Ji Zhu Lei Wang Bo-Hai Jiang Wei-Jie Zhao |
spellingShingle |
Ying-Qun Wang Shi-Sheng Wang Ji Zhu Lei Wang Bo-Hai Jiang Wei-Jie Zhao Determination of urea content in urea cream by centrifugal partition chromatography Journal of Food and Drug Analysis Centrifugal partition chromatography Content determination Urea cream |
author_facet |
Ying-Qun Wang Shi-Sheng Wang Ji Zhu Lei Wang Bo-Hai Jiang Wei-Jie Zhao |
author_sort |
Ying-Qun Wang |
title |
Determination of urea content in urea cream by centrifugal partition chromatography |
title_short |
Determination of urea content in urea cream by centrifugal partition chromatography |
title_full |
Determination of urea content in urea cream by centrifugal partition chromatography |
title_fullStr |
Determination of urea content in urea cream by centrifugal partition chromatography |
title_full_unstemmed |
Determination of urea content in urea cream by centrifugal partition chromatography |
title_sort |
determination of urea content in urea cream by centrifugal partition chromatography |
publisher |
Elsevier |
series |
Journal of Food and Drug Analysis |
issn |
1021-9498 |
publishDate |
2016-04-01 |
description |
The objective of this study is to establish a centrifugal partition chromatography (CPC) method for determination of the urea ingredient in urea cream. The mechanism of this method is that urea is determined by UV detector at 430 nm after being extracted from the cream and derivatized on line via Ehrlich reaction in rotor of CPC, where the reaction products dissolve in the mobile phase and the cream matrix retains in the stationary phase. The mixed solvent consisting of n-hexane, methanol, hydrochloric acid and p-dimethylaminobenzaldehyde with a ratio of 1000 mL:1000 mL:18 mL:2.0 g is used for solvent system of CPC. The CPC method proposed offers good precision and convenience without complex sample pretreatment processes. |
topic |
Centrifugal partition chromatography Content determination Urea cream |
url |
http://www.sciencedirect.com/science/article/pii/S1021949815001519 |
work_keys_str_mv |
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