Marker aided selection (MAS) for qualitative traits in segregating F2 populations of rice
The selection and screening of desirable traits at early stages of plant development is possible with the advancement of marker assisted selection (MAS). Marker aided selection can be used for monitoring the presence or absence of genes in breeding populations and can be combined with co...
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Serbian Genetics Society
2019-01-01
|
Series: | Genetika |
Subjects: | |
Online Access: | http://www.doiserbia.nb.rs/img/doi/0534-0012/2019/0534-00121903031H.pdf |
Summary: | The selection and screening of desirable traits at early stages of plant
development is possible with the advancement of marker assisted selection
(MAS). Marker aided selection can be used for monitoring the presence or
absence of genes in breeding populations and can be combined with
conventional breeding approaches. In this study, crosses between
high-yielding cultivar, Nemat, with 4 local aromatic varieties were made
followed by phenotypic selection for desirable individual plants in F2
populations. Then, MAS applied to genotypic selection of aromatic plants
using allele specific amplification (ASA) marker for fragrance (fgr) and SSR
marker RM190 linked with intermediate amylose content (AC), gel consistency
(GC) and gelatinization temperature (GT) in these populations. Molecular
screening based on RM190 and fragrance markers showed that 11 lines were
homozygous aromatic and 32 lines had desirable cooking quality in
segregating F2 population. Results demonstrated that marker assisted
selection in F2 segregating populations reduced the time and cost effective
for accelerating cultivar development in rice. Further analyses are underway
on these plants on subsequent generations through pedigree breeding method.
These plants promise to develop new aromatic rice lines through classical
and molecular breeding in the near future in Iran. |
---|---|
ISSN: | 0534-0012 1820-6069 |