Role of N-Arachidonoyl-Serotonin (AA-5-HT) in Sleep-Wake Cycle Architecture, Sleep Homeostasis, and Neurotransmitters Regulation
The endocannabinoid system comprises several molecular entities such as endogenous ligands [anandamide (AEA) and 2-arachidonoylglycerol (2-AG)], receptors (CB1 and CB2), enzymes such as [fatty acid amide hydrolase (FAHH) and monoacylglycerol lipase (MAGL)], as well as the anandamide membrane transpo...
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2017-05-01
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Online Access: | http://journal.frontiersin.org/article/10.3389/fnmol.2017.00152/full |
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Article |
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language |
English |
format |
Article |
sources |
DOAJ |
author |
Eric Murillo-Rodríguez Eric Murillo-Rodríguez Eric Murillo-Rodríguez Eric Murillo-Rodríguez Vincenzo Di Marzo Vincenzo Di Marzo Sergio Machado Sergio Machado Sergio Machado Nuno B. Rocha Nuno B. Rocha André B. Veras André B. Veras André B. Veras Geraldo A. M. Neto Geraldo A. M. Neto Henning Budde Henning Budde Henning Budde Henning Budde Oscar Arias-Carrión Oscar Arias-Carrión Gloria Arankowsky-Sandoval Gloria Arankowsky-Sandoval |
spellingShingle |
Eric Murillo-Rodríguez Eric Murillo-Rodríguez Eric Murillo-Rodríguez Eric Murillo-Rodríguez Vincenzo Di Marzo Vincenzo Di Marzo Sergio Machado Sergio Machado Sergio Machado Nuno B. Rocha Nuno B. Rocha André B. Veras André B. Veras André B. Veras Geraldo A. M. Neto Geraldo A. M. Neto Henning Budde Henning Budde Henning Budde Henning Budde Oscar Arias-Carrión Oscar Arias-Carrión Gloria Arankowsky-Sandoval Gloria Arankowsky-Sandoval Role of N-Arachidonoyl-Serotonin (AA-5-HT) in Sleep-Wake Cycle Architecture, Sleep Homeostasis, and Neurotransmitters Regulation Frontiers in Molecular Neuroscience sleep dopamine modafinil cannabidiol sleep deprivation |
author_facet |
Eric Murillo-Rodríguez Eric Murillo-Rodríguez Eric Murillo-Rodríguez Eric Murillo-Rodríguez Vincenzo Di Marzo Vincenzo Di Marzo Sergio Machado Sergio Machado Sergio Machado Nuno B. Rocha Nuno B. Rocha André B. Veras André B. Veras André B. Veras Geraldo A. M. Neto Geraldo A. M. Neto Henning Budde Henning Budde Henning Budde Henning Budde Oscar Arias-Carrión Oscar Arias-Carrión Gloria Arankowsky-Sandoval Gloria Arankowsky-Sandoval |
author_sort |
Eric Murillo-Rodríguez |
title |
Role of N-Arachidonoyl-Serotonin (AA-5-HT) in Sleep-Wake Cycle Architecture, Sleep Homeostasis, and Neurotransmitters Regulation |
title_short |
Role of N-Arachidonoyl-Serotonin (AA-5-HT) in Sleep-Wake Cycle Architecture, Sleep Homeostasis, and Neurotransmitters Regulation |
title_full |
Role of N-Arachidonoyl-Serotonin (AA-5-HT) in Sleep-Wake Cycle Architecture, Sleep Homeostasis, and Neurotransmitters Regulation |
title_fullStr |
Role of N-Arachidonoyl-Serotonin (AA-5-HT) in Sleep-Wake Cycle Architecture, Sleep Homeostasis, and Neurotransmitters Regulation |
title_full_unstemmed |
Role of N-Arachidonoyl-Serotonin (AA-5-HT) in Sleep-Wake Cycle Architecture, Sleep Homeostasis, and Neurotransmitters Regulation |
title_sort |
role of n-arachidonoyl-serotonin (aa-5-ht) in sleep-wake cycle architecture, sleep homeostasis, and neurotransmitters regulation |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Molecular Neuroscience |
issn |
1662-5099 |
publishDate |
2017-05-01 |
description |
The endocannabinoid system comprises several molecular entities such as endogenous ligands [anandamide (AEA) and 2-arachidonoylglycerol (2-AG)], receptors (CB1 and CB2), enzymes such as [fatty acid amide hydrolase (FAHH) and monoacylglycerol lipase (MAGL)], as well as the anandamide membrane transporter. Although the role of this complex neurobiological system in the sleep–wake cycle modulation has been studied, the contribution of the blocker of FAAH/transient receptor potential cation channel subfamily V member 1 (TRPV1), N-arachidonoyl-serotonin (AA-5-HT) in sleep has not been investigated. Thus, in the present study, varying doses of AA-5-HT (5, 10, or 20 mg/Kg, i.p.) injected at the beginning of the lights-on period of rats, caused no statistical changes in sleep patterns. However, similar pharmacological treatment given to animals at the beginning of the dark period decreased wakefulness (W) and increased slow wave sleep (SWS) as well as rapid eye movement sleep (REMS). Power spectra analysis of states of vigilance showed that injection of AA-5-HT during the lights-off period diminished alpha spectrum across alertness in a dose-dependent fashion. In opposition, delta power spectra was enhanced as well as theta spectrum, during SWS and REMS, respectively. Moreover, the highest dose of AA-5-HT decreased wake-related contents of neurotransmitters such as dopamine (DA), norepinephrine (NE), epinephrine (EP), serotonin (5-HT) whereas the levels of adenosine (AD) were enhanced. In addition, the sleep-inducing properties of AA-5-HT were confirmed since this compound blocked the increase in W caused by stimulants such as cannabidiol (CBD) or modafinil (MOD) during the lights-on period. Additionally, administration of AA-5-HT also prevented the enhancement in contents of DA, NE, EP, 5-HT and AD after CBD of MOD injection. Lastly, the role of AA-5-HT in sleep homeostasis was tested in animals that received either CBD or MOD after total sleep deprivation (TSD). The injection of CBD or MOD increased alertness during sleep rebound period after TSD. However, AA-5-HT blocked this effect by allowing animals to display an enhancement in sleep across sleep rebound period. Overall, our findings provide evidence that AA-5-HT is an important modulator of sleep, sleep homeostasis and neurotransmitter contents. |
topic |
sleep dopamine modafinil cannabidiol sleep deprivation |
url |
http://journal.frontiersin.org/article/10.3389/fnmol.2017.00152/full |
work_keys_str_mv |
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doaj-2c20ee70d75e4d8b88602fe3026d27732020-11-24T23:52:08ZengFrontiers Media S.A.Frontiers in Molecular Neuroscience1662-50992017-05-011010.3389/fnmol.2017.00152261917Role of N-Arachidonoyl-Serotonin (AA-5-HT) in Sleep-Wake Cycle Architecture, Sleep Homeostasis, and Neurotransmitters RegulationEric Murillo-Rodríguez0Eric Murillo-Rodríguez1Eric Murillo-Rodríguez2Eric Murillo-Rodríguez3Vincenzo Di Marzo4Vincenzo Di Marzo5Sergio Machado6Sergio Machado7Sergio Machado8Nuno B. Rocha9Nuno B. Rocha10André B. Veras11André B. Veras12André B. Veras13Geraldo A. M. Neto14Geraldo A. M. Neto15Henning Budde16Henning Budde17Henning Budde18Henning Budde19Oscar Arias-Carrión20Oscar Arias-Carrión21Gloria Arankowsky-Sandoval22Gloria Arankowsky-Sandoval23Laboratorio de Neurociencias Moleculares e Integrativas, Escuela de Medicina, División Ciencias de la Salud, Universidad Anáhuac MayabMérida, MexicoGrupo de Investigación en Envejecimiento, División Ciencias de la Salud, Universidad Anáhuac MayabMérida, MexicoGrupo de Investigación Desarrollos Tecnológicos para la Salud, División de Ingeniería y Ciencias Exactas, Universidad Anáhuac MayabMérida, Mexico Intercontinental Neuroscience Research Group Intercontinental Neuroscience Research GroupEndocannabinoid Research Group, Istituto di Chimica Biomolecolare, Consiglio Nazionale delle RicerchePozzuoli, Italy Intercontinental Neuroscience Research GroupLaboratory of Panic and Respiration, Institute of Psychiatry, Federal University of Rio de JaneiroRio de Janeiro, BrazilPostgraduate Program, Salgado de Oliveira UniversityRio de Janeiro, Brazil Intercontinental Neuroscience Research GroupFaculty of Health Sciences, Polytechnic Institute of PortoPorto, Portugal Intercontinental Neuroscience Research GroupInstitute of Psychiatry, Federal University of Rio de JaneiroRio de Janeiro, Brazil0Dom Bosco Catholic UniversityRio de Janeiro, Brazil Intercontinental Neuroscience Research GroupLaboratory of Panic and Respiration, Institute of Psychiatry, Federal University of Rio de JaneiroRio de Janeiro, Brazil Intercontinental Neuroscience Research Group1Faculty of Human Sciences, Medical School HamburgHamburg, Germany2Physical Activity, Physical Education, Health and Sport Research Centre (PAPESH), Sports Science Department, School of Science and Engineering Reykjavik UniversityReykjavik, Iceland3Department of Health, Physical and Social Education, Lithuanian Sports UniversityKaunas, Lithuania Intercontinental Neuroscience Research Group4Unidad de Trastornos del Movimiento y Sueño (TMS), Hospital General “Dr. Manuel Gea González"Ciudad de México, Mexico Intercontinental Neuroscience Research Group5Centro de Investigaciones Regionales “Dr. Hideyo Noguchi”, Universidad Autónoma de YucatánMérida, MexicoThe endocannabinoid system comprises several molecular entities such as endogenous ligands [anandamide (AEA) and 2-arachidonoylglycerol (2-AG)], receptors (CB1 and CB2), enzymes such as [fatty acid amide hydrolase (FAHH) and monoacylglycerol lipase (MAGL)], as well as the anandamide membrane transporter. Although the role of this complex neurobiological system in the sleep–wake cycle modulation has been studied, the contribution of the blocker of FAAH/transient receptor potential cation channel subfamily V member 1 (TRPV1), N-arachidonoyl-serotonin (AA-5-HT) in sleep has not been investigated. Thus, in the present study, varying doses of AA-5-HT (5, 10, or 20 mg/Kg, i.p.) injected at the beginning of the lights-on period of rats, caused no statistical changes in sleep patterns. However, similar pharmacological treatment given to animals at the beginning of the dark period decreased wakefulness (W) and increased slow wave sleep (SWS) as well as rapid eye movement sleep (REMS). Power spectra analysis of states of vigilance showed that injection of AA-5-HT during the lights-off period diminished alpha spectrum across alertness in a dose-dependent fashion. In opposition, delta power spectra was enhanced as well as theta spectrum, during SWS and REMS, respectively. Moreover, the highest dose of AA-5-HT decreased wake-related contents of neurotransmitters such as dopamine (DA), norepinephrine (NE), epinephrine (EP), serotonin (5-HT) whereas the levels of adenosine (AD) were enhanced. In addition, the sleep-inducing properties of AA-5-HT were confirmed since this compound blocked the increase in W caused by stimulants such as cannabidiol (CBD) or modafinil (MOD) during the lights-on period. Additionally, administration of AA-5-HT also prevented the enhancement in contents of DA, NE, EP, 5-HT and AD after CBD of MOD injection. Lastly, the role of AA-5-HT in sleep homeostasis was tested in animals that received either CBD or MOD after total sleep deprivation (TSD). The injection of CBD or MOD increased alertness during sleep rebound period after TSD. However, AA-5-HT blocked this effect by allowing animals to display an enhancement in sleep across sleep rebound period. Overall, our findings provide evidence that AA-5-HT is an important modulator of sleep, sleep homeostasis and neurotransmitter contents.http://journal.frontiersin.org/article/10.3389/fnmol.2017.00152/fullsleepdopaminemodafinilcannabidiolsleep deprivation |