New Role of P. brasiliensis α-Glucan: Differentiation of Non-conventional Dendritic Cells
The cell wall has a critical role in the host immune response to fungal pathogens. In this study, we investigated the influence of two cell wall fractions of the dimorphic fungi Paracoccidioides brasiliensis (Pb) in the in vitro generation of monocyte-derived dendritic cells (MoDCs). Monocytes were...
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doaj-2c4117cc07624ce39ccd930df54e29d52020-11-25T02:42:39ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-10-011010.3389/fmicb.2019.02445485143New Role of P. brasiliensis α-Glucan: Differentiation of Non-conventional Dendritic CellsAna Camila Oliveira Souza0Cecília Favali1Naiara Caroline Soares2Natalia Machado Tavares3Márcio Sousa Jerônimo4Paulo Henrique Veloso Junior5Clara Luna Marina6Claire Santos7Cláudia Brodskyn8Anamelia Lorenzetti Bocca9Departamento de Biologia Celular, Universidade de Brasília, Brasília, BrazilDepartamento de Biologia Celular, Universidade de Brasília, Brasília, BrazilDepartamento de Biologia Celular, Universidade de Brasília, Brasília, BrazilCentro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, BrazilDepartamento de Biologia Celular, Universidade de Brasília, Brasília, BrazilDepartamento de Biologia Celular, Universidade de Brasília, Brasília, BrazilDepartamento de Biologia Celular, Universidade de Brasília, Brasília, BrazilCentro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, BrazilCentro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, BrazilDepartamento de Biologia Celular, Universidade de Brasília, Brasília, BrazilThe cell wall has a critical role in the host immune response to fungal pathogens. In this study, we investigated the influence of two cell wall fractions of the dimorphic fungi Paracoccidioides brasiliensis (Pb) in the in vitro generation of monocyte-derived dendritic cells (MoDCs). Monocytes were purified from the peripheral blood of healthy donors and cultivated for 7 days in medium supplemented with IL-4 and GM-CSF in the presence of Pb cell wall fractions: the alkali-insoluble F1, constituted by β-1,3-glucans, chitin and proteins, and the alkali-soluble F2, mainly constituted by α-glucan. MoDCs phenotypes were evaluated regarding cell surface expression of CD1a, DC-SIGN, HLA-DR, CD80, and CD83 and production of cytokines. The α-glucan-rich cell wall fraction downregulated the differentiation of CD1a+ MoDCs, a dendritic cell subset that stimulate Th1 responses. The presence of both cell fractions inhibited DC-SIGN and HLA-DR expression, while the expression of maturation markers was differentially induced in CD1a– MoDCs. Differentiation upon F1 and F2 stimulation induced mixed profile of inflammatory cytokines. Altogether, these data demonstrate that Pb cell wall fractions differentially induce a dysregulation in DCs differentiation. Moreover, our results suggest that cell wall α-glucan promote the differentiation of CD1a– DCs, potentially favoring Th2 polarization and contributing to pathogen persistence.https://www.frontiersin.org/article/10.3389/fmicb.2019.02445/fullParacoccidiodomycosisParacoccidioides brasiliensisdendritic cell differentiationglucancell wall fractions |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ana Camila Oliveira Souza Cecília Favali Naiara Caroline Soares Natalia Machado Tavares Márcio Sousa Jerônimo Paulo Henrique Veloso Junior Clara Luna Marina Claire Santos Cláudia Brodskyn Anamelia Lorenzetti Bocca |
spellingShingle |
Ana Camila Oliveira Souza Cecília Favali Naiara Caroline Soares Natalia Machado Tavares Márcio Sousa Jerônimo Paulo Henrique Veloso Junior Clara Luna Marina Claire Santos Cláudia Brodskyn Anamelia Lorenzetti Bocca New Role of P. brasiliensis α-Glucan: Differentiation of Non-conventional Dendritic Cells Frontiers in Microbiology Paracoccidiodomycosis Paracoccidioides brasiliensis dendritic cell differentiation glucan cell wall fractions |
author_facet |
Ana Camila Oliveira Souza Cecília Favali Naiara Caroline Soares Natalia Machado Tavares Márcio Sousa Jerônimo Paulo Henrique Veloso Junior Clara Luna Marina Claire Santos Cláudia Brodskyn Anamelia Lorenzetti Bocca |
author_sort |
Ana Camila Oliveira Souza |
title |
New Role of P. brasiliensis α-Glucan: Differentiation of Non-conventional Dendritic Cells |
title_short |
New Role of P. brasiliensis α-Glucan: Differentiation of Non-conventional Dendritic Cells |
title_full |
New Role of P. brasiliensis α-Glucan: Differentiation of Non-conventional Dendritic Cells |
title_fullStr |
New Role of P. brasiliensis α-Glucan: Differentiation of Non-conventional Dendritic Cells |
title_full_unstemmed |
New Role of P. brasiliensis α-Glucan: Differentiation of Non-conventional Dendritic Cells |
title_sort |
new role of p. brasiliensis α-glucan: differentiation of non-conventional dendritic cells |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2019-10-01 |
description |
The cell wall has a critical role in the host immune response to fungal pathogens. In this study, we investigated the influence of two cell wall fractions of the dimorphic fungi Paracoccidioides brasiliensis (Pb) in the in vitro generation of monocyte-derived dendritic cells (MoDCs). Monocytes were purified from the peripheral blood of healthy donors and cultivated for 7 days in medium supplemented with IL-4 and GM-CSF in the presence of Pb cell wall fractions: the alkali-insoluble F1, constituted by β-1,3-glucans, chitin and proteins, and the alkali-soluble F2, mainly constituted by α-glucan. MoDCs phenotypes were evaluated regarding cell surface expression of CD1a, DC-SIGN, HLA-DR, CD80, and CD83 and production of cytokines. The α-glucan-rich cell wall fraction downregulated the differentiation of CD1a+ MoDCs, a dendritic cell subset that stimulate Th1 responses. The presence of both cell fractions inhibited DC-SIGN and HLA-DR expression, while the expression of maturation markers was differentially induced in CD1a– MoDCs. Differentiation upon F1 and F2 stimulation induced mixed profile of inflammatory cytokines. Altogether, these data demonstrate that Pb cell wall fractions differentially induce a dysregulation in DCs differentiation. Moreover, our results suggest that cell wall α-glucan promote the differentiation of CD1a– DCs, potentially favoring Th2 polarization and contributing to pathogen persistence. |
topic |
Paracoccidiodomycosis Paracoccidioides brasiliensis dendritic cell differentiation glucan cell wall fractions |
url |
https://www.frontiersin.org/article/10.3389/fmicb.2019.02445/full |
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