Molecular phylogeny of Artemisia species based on the internal transcribed spacer (ITS) of 18S-26S rDNA in Ordu Province of Turkey

• Main Authors: Onur Koloren, Zeynep Koloren, Seçil Eker
• Format: Article
• Language: English
• Published: Taylor & Francis Group 2016-09-01
• Series: Biotechnology & Biotechnological Equipment
• Subjects: Artemisia; rDNA-ITS; molecular phylogeny
• Online Access: http://dx.doi.org/10.1080/13102818.2016.1188674

Economically important representatives of the Artemisia genus have wide application in pharmaceutics, landscape architecture and agriculture. The aim of this study was to phylogenetically analyse the 18S-26S rDNA, the internal transcribed spacer (ITS) nucleotide sequences of 19 Artemisia samples collected from Ordu Province of Turkey. Analyses were conducted using neighbour-joining (NJ), maximum-parsimony (MP) and maximum-likelihood (ML) algorithms. Our analysis revealed two unique haplotypes within our samples, including a rare one (Haplotype-I, represented by a single sample) and a common one (Haplotype-II, represented by 18 samples). In all trees, both of our haplotypes appeared in the same lineage with Artemisia sylvatica, Artemisia argyi and Artemisia verlotiorum. Although rDNA-ITS is known to be a variative marker, oddly it was highly conserved in Artemisia, which is why we used this marker to determine the phylogenetic affiliation of the analysed plants.