Genetic Characterisation and Comparison of Three Human Coronaviruses (HKU1, OC43, 229E) from Patients and Bovine Coronavirus (BCoV) from Cattle with Respiratory Disease in Slovenia
Coronaviruses (CoV) are widely distributed pathogens of human and animals and can cause mild or severe respiratory and gastrointestinal disease. Antigenic and genetic similarity of some CoVs within the <i>Betacoronavirus</i> genus is evident. Therefore, for the first time in Slovenia, we...
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doaj-2c507520f4f44750a54ee76be25e8b272021-04-15T23:01:15ZengMDPI AGViruses1999-49152021-04-011367667610.3390/v13040676Genetic Characterisation and Comparison of Three Human Coronaviruses (HKU1, OC43, 229E) from Patients and Bovine Coronavirus (BCoV) from Cattle with Respiratory Disease in SloveniaMonika Jevšnik Virant0Danijela Černe1Miroslav Petrovec2Tomislav Paller3Ivan Toplak4Institute of Microbiology and Immunology, Faculty of Medicine, University of Ljubljana, Zaloška 4, 1000 Ljubljana, SloveniaVirology Unit, Institute of Microbiology and Parasitology, Veterinary Faculty, University of Ljubljana, Gerbičeva 60, 1115 Ljubljana, SloveniaInstitute of Microbiology and Immunology, Faculty of Medicine, University of Ljubljana, Zaloška 4, 1000 Ljubljana, SloveniaNational Veterinary Institute, Veterinary Faculty, University of Ljubljana, Gerbičeva 60, 1115 Ljubljana, SloveniaVirology Unit, Institute of Microbiology and Parasitology, Veterinary Faculty, University of Ljubljana, Gerbičeva 60, 1115 Ljubljana, SloveniaCoronaviruses (CoV) are widely distributed pathogens of human and animals and can cause mild or severe respiratory and gastrointestinal disease. Antigenic and genetic similarity of some CoVs within the <i>Betacoronavirus</i> genus is evident. Therefore, for the first time in Slovenia, we investigated the genetic diversity of partial 390-nucleotides of RNA-dependent-RNA polymerase gene (RdRp) for 66 human (HCoV) and 24 bovine CoV (BCoV) positive samples, collected between 2010 and 2016 from human patients and cattle with respiratory disease. The characterized CoV strains belong to four different clusters, in three separate human clusters HCoV-HKU1 (<i>n</i> = 34), HCoV-OC43 (<i>n</i> = 31) and HCoV 229E (<i>n</i> = 1) and bovine grouping only as BCoVs (<i>n</i> = 24). BCoVs from cattle and HCoV-OC43 were genetically the most closely related and share 96.4–97.1% nucleotide and 96.9–98.5% amino acid identity.https://www.mdpi.com/1999-4915/13/4/676coronavirusesgenetic diversityHCoV-OC43BCoVtransmission |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Monika Jevšnik Virant Danijela Černe Miroslav Petrovec Tomislav Paller Ivan Toplak |
spellingShingle |
Monika Jevšnik Virant Danijela Černe Miroslav Petrovec Tomislav Paller Ivan Toplak Genetic Characterisation and Comparison of Three Human Coronaviruses (HKU1, OC43, 229E) from Patients and Bovine Coronavirus (BCoV) from Cattle with Respiratory Disease in Slovenia Viruses coronaviruses genetic diversity HCoV-OC43 BCoV transmission |
author_facet |
Monika Jevšnik Virant Danijela Černe Miroslav Petrovec Tomislav Paller Ivan Toplak |
author_sort |
Monika Jevšnik Virant |
title |
Genetic Characterisation and Comparison of Three Human Coronaviruses (HKU1, OC43, 229E) from Patients and Bovine Coronavirus (BCoV) from Cattle with Respiratory Disease in Slovenia |
title_short |
Genetic Characterisation and Comparison of Three Human Coronaviruses (HKU1, OC43, 229E) from Patients and Bovine Coronavirus (BCoV) from Cattle with Respiratory Disease in Slovenia |
title_full |
Genetic Characterisation and Comparison of Three Human Coronaviruses (HKU1, OC43, 229E) from Patients and Bovine Coronavirus (BCoV) from Cattle with Respiratory Disease in Slovenia |
title_fullStr |
Genetic Characterisation and Comparison of Three Human Coronaviruses (HKU1, OC43, 229E) from Patients and Bovine Coronavirus (BCoV) from Cattle with Respiratory Disease in Slovenia |
title_full_unstemmed |
Genetic Characterisation and Comparison of Three Human Coronaviruses (HKU1, OC43, 229E) from Patients and Bovine Coronavirus (BCoV) from Cattle with Respiratory Disease in Slovenia |
title_sort |
genetic characterisation and comparison of three human coronaviruses (hku1, oc43, 229e) from patients and bovine coronavirus (bcov) from cattle with respiratory disease in slovenia |
publisher |
MDPI AG |
series |
Viruses |
issn |
1999-4915 |
publishDate |
2021-04-01 |
description |
Coronaviruses (CoV) are widely distributed pathogens of human and animals and can cause mild or severe respiratory and gastrointestinal disease. Antigenic and genetic similarity of some CoVs within the <i>Betacoronavirus</i> genus is evident. Therefore, for the first time in Slovenia, we investigated the genetic diversity of partial 390-nucleotides of RNA-dependent-RNA polymerase gene (RdRp) for 66 human (HCoV) and 24 bovine CoV (BCoV) positive samples, collected between 2010 and 2016 from human patients and cattle with respiratory disease. The characterized CoV strains belong to four different clusters, in three separate human clusters HCoV-HKU1 (<i>n</i> = 34), HCoV-OC43 (<i>n</i> = 31) and HCoV 229E (<i>n</i> = 1) and bovine grouping only as BCoVs (<i>n</i> = 24). BCoVs from cattle and HCoV-OC43 were genetically the most closely related and share 96.4–97.1% nucleotide and 96.9–98.5% amino acid identity. |
topic |
coronaviruses genetic diversity HCoV-OC43 BCoV transmission |
url |
https://www.mdpi.com/1999-4915/13/4/676 |
work_keys_str_mv |
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